A chimeric vaccine targeting Pseudomonas aeruginosa virulence factors protects mice against lethal infection.

Pseudomonas aeruginosa is an important and hazardous nosocomial pathogen in respiratory tract infections and rapidly achieves antibiotic resistance, so it is necessary to develop an effective vaccine to combat the infection. The Type III secretion system (T3SS) protein P. aeruginosa V-antigen (PcrV), outer membrane protein F (OprF), and two kinds of flagellins (FlaA and FlaB) all play important roles in the pathogenesis of P.

A trivalent vaccine consisting of "flagellin A+B and pilin" protects against Pseudomonas aeruginosa infection in a murine burn model.

Pseudomonas aeruginosa is a common nosocomial pathogen in burn patients, and rapidly achieves antibiotic resistance, and thus, developing an effective vaccine is critically important for combating P. aeruginosa infection. Flagella and pili play important roles in colonization of P.

Passive immunization against Pseudomonas aeruginosa recombinant PilA in a murine burn wound model.

Pseudomonas aeruginosa type IV pili have an essential role in twitching motility, colonization and biofilm formation. In this study, we investigated the efficacy of intraperitoneal administration of rabbit anti-recombinant PilA (anti-r-PilA) immunoglobulin G (IgG) against P. aeruginosa infection in a mouse burn-wound model.

Flagellin and pilin immunization against multi-drug resistant Pseudomonas aeruginosa protects mice in the burn wound sepsis model.

Pseudomonas aeruginosa is a formidable pathogen and a major threat to burn patients. Antimicrobial therapy is often unsuccessful because P. aeruginosa can develop multi-drug resistance; thus, immunotherapy and vaccine can be a rational alternative.

Immunogenicity and protective efficacy of Pseudomonas aeruginosa type a and b flagellin vaccines in a burned mouse model.

Immunogenicity and efficacy of Pseudomonas aeruginosa type a and b flagellins (hereafter, flagellins) as candidate vaccines were evaluated using an experimental burned mouse model. The protection afforded and the reduction in bacterial burden achieved by these vaccine candidates were determined. Primary immunization with flagellins followed by two booster shots generated a robust immune response.

Active Immunization with Recombinant PilA protein Protects Against Pseudomonas aeruginosa Infection in a Mouse Burn Wound Model.

Pseudomonas aeruginosa as an opportunistic human pathogen that causes lethal infections in immunocompromised patients. Type IV pili are critical factors in virulence and colonization of P. aeruginosa in acute burn wound infection.