Bidirectional transcription at the gene locus in spinocerebellar ataxia type 12.

Objective: Spinocerebellar ataxia type 12 (SCA12) is a neurodegenerative disease caused by expansion of a CAG repeat in the . Here we tested the hypothesis that the ( ) transcript containing a CUG repeat is expressed and contributes to SCA12 pathogenesis.

Methods: Expression of transcript was detected in SCA12 human induced pluripotent stem cells (iPSCs), iPSC-derived NGN2 neurons, and SCA12 knock-in mouse brains using strand-specific RT-PCR (SS-RT-PCR).