MiRNA Isolation from Plants Rich in Polysaccharides and Polyphenols.

MicroRNAs (miRNAs) are a group of 18-22-nucleotide-long noncoding RNAs, which show wide array of roles in various biological and metabolic processes in both animals and plants. They are formed endogenously and have evolutionary conserved sequences. RNA quality is extremely important for sequencing miRNAs using next-generation sequencing platforms.

High-quality RNA extraction from small cardamom tissues rich in polysaccharides and polyphenols.

Due to the presence of a diverse array of metabolites, no standard method of RNA isolation is available for plants. We noted that polysaccharide and polyphenol contents of cardamom tissues critically hinder the RNA extraction procedure. Hence, we attempted several methods for obtaining intact mRNA and small RNA from various cardamom tissues.