Risk Adjustment in Medical Research: A Bird's Eye View.

Making correct decisions is of paramount importance in clinical medicine and health-related disciplines. Randomized clinical trials are considered the gold-standard type of study for the assessment of the efficacy of a treatment. However, conducting a randomized clinical trial is not always possible; observational studies should be conducted, instead.

The effect on the equilibrium sickle cell allele frequency of the probable protection conferred by malaria and sickle cell gene against other infectious diseases.

If a mutated gene with heterozygous advantage against malaria, e.g., hemoglobin S (HbS) gene, is introduced in a small tribe, the gene (allele) frequency (f) increases until it reaches a steady state value (f) where the total mortality from malaria and sickle cell disease is a minimum.

Reinterpretation of the results of randomized clinical trials.

Background: Randomized clinical trials (RCTs) shape our clinical practice. Several studies report a mediocre replicability rate of the studied RCTs. Many researchers believe that the relatively low replication rate of RCTs is attributed to the high p value significance threshold.

On the feasibility of malaria hypothesis.

In 1954, Allison proposed that hemoglobin S (HbS) gene causes protection against fatal malaria. This would explain the high HbS gene frequency observed in certain regions hyperendemic for malaria, so-called "malaria hypothesis". This in silico study was conducted to examine the feasibility of the hypothesis under more realistic initial conditions, where a mutant gene with heterozygous advantage against malaria (e.

Data Distribution: Normal or Abnormal?

Determining if the frequency distribution of a given data set follows a normal distribution or not is among the first steps of data analysis. Visual examination of the data, commonly by Q-Q plot, although is acceptable by many scientists, is considered subjective and not acceptable by other researchers. One-sample Kolmogorov-Smirnov test with Lilliefors correction (for a sample size ≥ 50) and Shapiro-Wilk test (for a sample size < 50) are common statistical tests for checking the normality of a data set quantitatively.

On the use of receiver operating characteristic curve analysis to determine the most appropriate p value significance threshold.

Background: p value is the most common statistic reported in scientific research articles. Choosing the conventional threshold of 0.05 commonly used for the p value in research articles, is unfounded.

Chatbots, generative AI, and scholarly manuscripts: WAME recommendations on chatbots and generative artificial intelligence in relation to scholarly publications.

This statement revises our earlier "WAME Recommendations on ChatGPT and Chatbots in Relation to Scholarly Publications" (January 20, 2023). The revision reflects the proliferation of chatbots and their expanding use in scholarly publishing over the last few months, as well as emerging concerns regarding lack of authenticity of content when using chatbots. These recommendations are intended to inform editors and help them develop policies for the use of chatbots in papers published in their journals.

Plagiarism: A Bird's Eye View.

Plagiarism is among the prevalent misconducts reported in scientific writing and common causes of article retraction in scholarly journals. Plagiarism of idea is not acceptable by any means. However, plagiarism of text is a matter of debate from culture to culture.

GPTZero Performance in Identifying Artificial Intelligence-Generated Medical Texts: A Preliminary Study.

Background: With emergence of chatbots to help authors with scientific writings, editors should have tools to identify artificial intelligence-generated texts. GPTZero is among the first websites that has sought media attention claiming to differentiate machine-generated from human-written texts.

Methods: Using 20 text pieces generated by ChatGPT in response to arbitrary questions on various topics in medicine and 30 pieces chosen from previously published medical articles, the performance of GPTZero was assessed.

The Acceptable Text Similarity Level in Manuscripts Submitted to Scientific Journals.

Plagiarism is among commonly identified scientific misconducts in submitted manuscripts. Some journals routinely check the level of text similarity in the submitted manuscripts at the time of submission and reject the submission on the fly if the text similarity score exceeds a set cut-off value (, 20%). Herein, I present a manuscript with 32% text similarity, yet without any instances of text plagiarism.

When to Attend a Webinar?

Objective: To determine the most appropriate delay to start a webinar.

Methods: This cross-sectional study was conducted on weekly general staff scientific webinars held by the Institute of Human Virology (IHV), University of Maryland School of Medicine, Baltimore, USA. 35 observations were made at arbitrary chosen times of three consecutive IHV webinars.

Correction of vaccine effectiveness derived from test-negative case-control studies.

Background: Determining the vaccine effectiveness (VE) is an important part of studying every new vaccine. Test-negative case-control (TNCC) studies have recently been used to determine the VE. However, the estimated VE derived from a TNCC design depends on the test sensitivity and specificity.

Malaria and the incidence of COVID-19 in Africa: an ecological study.

Background: It has been shown that stimulation of innate immunity may provide temporary protection against a variety of infectious diseases. Malaria has been shown to induce a robust innate immune response. This study was conducted to test the hypothesis that if the cumulative number of cases diagnosed with COVID-19 per 100,000 population was correlated with the prevalence of malaria in African countries where both malaria and COVID-19 were prevalent.

No need for a gold-standard test: on the mining of diagnostic test performance indices merely based on the distribution of the test value.

Background: Diagnostic tests are important in clinical medicine. To determine the test performance indices - test sensitivity, specificity, likelihood ratio, predictive values, etc. - the test results should be compared against a gold-standard test.

On determining the sensitivity and specificity of a new diagnostic test through comparing its results against a non-gold-standard test.

Diagnostic tests are important clinical tools. To assess the sensitivity and specificity of a new test, its results should be compared against a gold standard. However, the gold-standard test is not always available.

On Measuring Vaccine Effectiveness with Observational Study Designs.

Herein, we present a bird's eye view of common observational study designs utilized for measurement of vaccine effectiveness. Assessing vaccines effectiveness is an integral part of vaccine research, particularly for the newly developed vaccines. A cohort study is prospective, directing from an exposure to one or more outcomes.

Use of Oral Polio Vaccine and the Global Incidence of Mother-to-Child Human Immunodeficiency Virus Transmission.

Background: Mother-to-child transmission (MTCT) of human immunodeficiency virus (HIV) is an important global health issue. We hypothesized that the live attenuated poliovirus existing in oral polio vaccine (OPV) may protect uninfected neonates born to HIV-positive mothers through the stimulation of innate immune system.

Objective: To test the hypothesis that countries using OPV have a lower MTCT rate (due to postnatal protection provided by the vaccine) compared with those using only inactivated polio vaccine (IPV).

The apparent prevalence, the true prevalence.

Serologic tests are important for conducting seroepidemiologic and prevalence studies. However, the tests used are typically imperfect and produce false-positive and false-negative results. This is why the seropositive rate (apparent prevalence) does not typically reflect the true prevalence of the disease or condition of interest.

Determining the SARS-CoV-2 serological immunoassay test performance indices based on the test results frequency distribution.

Introduction: Coronavirus disease 2019 (COVID-19) is known to induce robust antibody response in most of the affected individuals. The objective of the study was to determine if we can harvest the test sensitivity and specificity of a commercial serologic immunoassay merely based on the frequency distribution of the SARS-CoV-2 immunoglobulin (Ig) G concentrations measured in a population-based seroprevalence study.

Materials And Methods: The current study was conducted on a subset of a previously published dataset from the canton of Geneva.