Hair and urinary 2-hydroxynaphthalene levels in the people living in a region with frequent oil pipeline incidents in Iran: Health risk assessment.

Oil spills from pipeline accidents can have long-lasting health effects on residents of polluted regions. Assessing the potential health risk of these accidents is crucial for effective environmental health management. This study analyzed the concentration of 2-OHNAP in urine and hair as biomarkers of PAHs exposure among the people living in a region with frequent oil pipeline incident in Iran.

Co-occurrence of polycyclic aromatic hydrocarbons and heavy metals in various environmental matrices of a chronic petroleum polluted region in Iran; Pollution characterization, and assessment of ecological and human health risks.

Oil spills from pipeline accidents can result in long-lasting health effect in the people living in a ‎polluted region‎. In this study, the level of the 16 US EPA priority polycyclic aromatic hydrocarbons (PAHs‎) and heavy metals (HMs) have been ‎analyzed in environmental matrices of a region with frequent oil ‎pipeline accidents in Iran‎.‎ The results showed that the mean ‎concentration of ΣPAHs and ΣHMs decreased from the upstream to the downstream and also the levels were ‎higher in the wet season than those in the dry season.

Melamine sponges incorporated azo-linked porous organic polymer as adsorbent for extraction and determination of six B vitamins using pipette tip micro solid-phase extraction.

A novel azo-linked porous organic polymer (AL-POP) was synthesized from caffeic acid and benzidine via an azo-coupling reaction and characterized by FTIR, SEM-EDS, BET, TGA, XRD and zeta potential analysis. AL-POPs were incorporated into melamine sponges and used for pipette tip micro solid-phase extraction (PT-MSPE) of six types of B vitamins (including thiamine, riboflavin, nicotinamide, pyridoxine, folic acid, and cyanocobalamin). After extraction, the samples were analyzed using high performance liquid chromatography-diode array detection (HPLC-DAD) system.

Indole-Containing Metal Complexes and Their Medicinal Applications.

Indole is an important element of many natural and synthetic molecules with significant biological activity. Nonetheless, the co-presence of transitional metals in organic scaffold may represent an important factor in the development of effective medicinal agents. This review covers some of the latest and most relevant achievements in the biological and pharmacological activity of important indole-containing metal complexes in the area of drug discovery.

Biomass valorization: Sulfated lignin-catalyzed production of 5-hydroxymethylfurfural from fructose.

This study is aimed at the investigation of the preparation of sulfated lignin (SL) as a Bronsted acid catalyst for the preparation of 5-hydroxymethylfurfural (5-HMF). SL was characterized by different methods including FT-IR, FESEM, XRD, and EDS analyses. It shows promising results after 60 min of reaction at 140 °C, reaching 100% conversion of fructose precursor and 99% yield of 5-HMF, with a fructose: catalyst mass ratio of 10:6.

DNA and HSA interaction of Vanadium (IV), Copper (II), and Zinc (II) complexes derived from an asymmetric bidentate Schiff-base ligand: multi spectroscopic, viscosity measurements, molecular docking, and ONIOM studies.

The interaction of three complexes [Zn(II), Cu(II), and V(IV)] derived from an asymmetric bidentate Schiff-base ligand with DNA and HSA was studied using fluorescence quenching, UV-Vis spectroscopy, viscosity measurements, and computational methods [molecular docking and our Own N-layered Integrated molecular Orbital and molecular Mechanics (ONIOM)]. The obtained results revealed that the DNA and HSA affinities for binding of the synthesized compounds follow as V(IV) > Zn(II) > Cu(II) and Zn(II) > V(IV) > Cu(II), respectively. The distance between these compounds and HSA was obtained based on the Förster's theory of non-radiative energy transfer.

Metabolic engineering of Saccharomyces cerevisiae for linalool production.

Objectives: To engineer the yeast Saccharomyces cerevisiae for the heterologous production of linalool.

Results: Expression of linalool synthase gene from Lavandula angustifolia enabled heterologous production of linalool in S. cerevisiae.

Application of methyl silane coated iron oxide magnetic nanoparticles for solid-phase extraction and determination of fat-soluble vitamins by high performance liquid chromatography.

Methyl silane coated Fe3O4 magnetic nanoparticles were used for simultaneous extraction of the fat-soluble vitamins (FSVs). The amounts of extracted vitamins were determined by HPLC. The synthesized Fe3O4 nanoparticles were coated with silica and then modified with trimethoxymethylsilane (TMMS).

Toluene removal from sandy soils via in situ technologies with an emphasis on factors influencing soil vapor extraction.

The integration of bioventing (BV) and soil vapor extraction (SVE) appears to be an effective combination method for soil decontamination. This paper serves two main purposes: it evaluates the effects of soil water content (SWC) and air flow rate on SVE and it investigates the transition regime between BV and SVE for toluene removal from sandy soils. 96 hours after air injection, more than 97% removal efficiency was achieved in all five experiments (carried out for SVE) including 5, 10, and 15% for SWC and 250 and 500 mL/min for air flow rate on SVE.

Application of Glycyrrhiza glabra root as a novel adsorbent in the removal of toluene vapors: equilibrium, kinetic, and thermodynamic study.

The aim of this paper is to investigate the removal of toluene from gaseous solution through Glycyrrhiza glabra root (GGR) as a waste material. The batch adsorption experiments were conducted at various conditions including contact time, adsorbate concentration, humidity, and temperature. The adsorption capacity was increased by raising the sorbent humidity up to 50 percent.

Conversion of isoeugenol to vanillin by Psychrobacter sp. strain CSW4.

To screen strains of halotolerant or halophile bacteria which are able to convert isoeugenol to vanillin, 36 different strains of bacteria isolated from the salty environments in Iran were investigated. During growth on isoeugenol, a moderately halotolerant Gram-negative coccobacil showed capability of converting isoeugenol to vanillin. Based on morphological, physiological, and phylogenetic studies, strain CSW4 was classified as a bacterium belonging to the genus Psychrobacter.

Use of Growing Cells of Pseudomonas aeruginosa for Synthesis of the Natural Vanillin via Conversion of Isoeugenol.

The great demand of people for consumption of natural additives resulted in producing natural vanillin. There are plant sources and chemical procedures for vanillin production but microbial bioconversions are being sought as a suitable alternative. In the present work, the ability to produce vanillin from isoeugenol was screened using growing cultures of various bacteria.

Pseudomonas resinovorans SPR1, a newly isolated strain with potential of transforming eugenol to vanillin and vanillic acid.

In this study a novel strain was isolated with the capability to grow on eugenol as a source of carbon and energy. This strain was identified as Pseudomonas resinovorans (GenBank accession no. HQ198585) based on phenotypic characterization and phylogenetic analysis of 16S rDNA gene.

Candida galli strain PGO6: a novel isolated yeast strain capable of transformation of isoeugenol into vanillin and vanillic acid.

Candida galli strain PGO6 isolated from oil-contaminated water is the first isolated yeast strain which is capable to form vanillin and vanillic acid during isoeugenol biotransformation. The products were confirmed by thin-layer chromatography (TLC), changes in the UV absorption pattern and high-performance liquid chromatography (HPLC). The phenotypic and physiochemical characteristics as well as molecular phylogenetic analysis based on amplification the ITS1-5.

Simultaneous microemulsion liquid chromatographic analysis of fat-soluble vitamins in pharmaceutical formulations: optimization using genetic algorithm.

An environmentally benign and simple method has been proposed for separation and determination of fat-soluble vitamins using isocratic microemulsion liquid chromatography. Optimization of parameters affecting the separation selectivity and efficiency including surfactant concentration, percent of cosurfactant (1-butanol), and percent of organic oily solvent (diethyl ether), temperature and pH were performed simultaneously using genetic algorithm method. A new software package, MLR-GA, was developed for this purpose.

Effect of plant oils upon lipase and citric acid production in Yarrowia lipolytica yeast.

The nonconventional yeast Yarrowia lipolytica degrades very efficiently hydrophobic substrates to produce organic acids, single-cell oil, lipases, and so forth. The aim of this study was to investigate the biochemical behavior and simultaneous production of valuable metabolites such as lipase, citric acid (CA), and single-cell protein (SCP) by Yarrowia lipolytica DSM 3286 grown on various plant oils as sole carbon source. Among tested plant oils, olive oil proved to be the best medium for lipase and CA production.

Sensitive determination of carbohydrates labelled with p-nitroaniline by capillary electrophoresis with photometric detection using a 406 nm light-emitting diode.

p-Nitroaniline was explored as a derivatising reagent for UV absorbance detection of carbohydrates after separation by CE. This derivatising agent has three advantages: first, it has excellent water solubility; second, it has high molar absorptivity; and third, it is possible to obtain sensitive detection using a UV or blue light-emitting diode (LED) as the light source. The labelling reaction took less than 30 min to complete with high reaction yield.

Separation and determination of sugars by reversed-phase high-performance liquid chromatography after pre-column microwave-assisted derivatization.

In this study the possibility of derivatizing sugars using microwave irradiation was investigated. The amount of reagent, irradiation intensity, and derivatization time were optimized. In the derivatization of sugars with p-nitroaniline the reaction is complete within 5 min at 600 W when the p-nitroaniline-to-sugar and NaBH3CN-to-sugar mole ratios were above 1.

Separation of B6 vitamers with micellar liquid chromatography using UV and electrochemical detection.

Separation of six vitamers of vitamin B6 was performed by RP-HPLC using micellar mobile phase, UV and electrochemical detection. Effect of temperature, type and amount of organic modifier in mobile phase on efficiency and asymmetry factor showed that, the appropriate conditions were temperature of 35 degrees C and 3.0-5.

Simultaneous micellar liquid chromatographic analysis of seven water-soluble vitamins: optimization using super-modified simplex.

A super-modified simplex (SMS) method has been used to optimize the mobile phase used for separation of seven water-soluble vitamins in multivitamin tablets by gradient micellar liquid chromatography (MLC) with ultraviolet (UV) detection at 254, 295, and 361 nm. Effect of column temperature and addition of organic modifier to the mobile phase on separation efficiency were investigated: the appropriate conditions used were a temperature of 35 degrees C and 1-butanol modifier. The sodium dodecyl sulfate (SDS) concentration, pH, and 1-butanol% in the mobile phase were chosen for simultaneous optimization using the SMS method.