Inducible immortality in hTERT-human mesenchymal stem cells.

Human mesenchymal stem cells (hMSCs) are attractive candidates for tissue engineering and cell-based therapy because of their multipotentiality and availability in adult donors. However, in vitro expansion and differentiation of these cells is limited by replicative senescence. The proliferative capacity of hMSCs can be enhanced by ectopic expression of telomerase, allowing for long-term culture.

Does limb-salvage surgery offer patients better quality of life and functional capacity than amputation?

Introduction: Patients with aggressive lower extremity musculoskeletal tumors may be candidates for either above-knee amputation or limb-salvage surgery. However, the subjective and objective benefits of limb-salvage surgery compared with amputation are not fully clear.

Questions/purposes: We therefore compared functional status and quality of life for patients treated with above-knee amputation versus limb-salvage surgery.

Relaxation effects of ferucarbotran-labeled mesenchymal stem cells at 1.5T and 3T: discrimination of viable from lysed cells.

Human mesenchymal stem cells (hMSCs) were labeled with Ferucarbotran by simple incubation and cultured for up to 14 d. Iron content was determined by spectrometry and the intracellular localization of the contrast agent uptake was studied by electron and confocal microscopy. At various time points after labeling, ranging from 1 to 14 d, samples with viable or lysed labeled hMSCs, as well as nonlabeled controls, underwent MRI.

The functional anaesthetic discogram: description of a novel diagnostic technique and report of 3 cases.

Background: The diagnostic evaluation of patients with presumed discogenic low back pain is controversial; recent studies have brought the specificity of the traditional technique, provocative lumbar discography, into question. One of the explanations for the relative lack of predictability in treatment outcomes for patients with discogenic low back pain may be a corresponding lack of certainty in the diagnosis.

Purpose: A new diagnostic technique is described for the evaluation of patients with presumptive discogenic low back pain; the cases of 3 patients in whom the technique was used are presented.

Clostridium cadaveris septic arthritis after total hip arthroplasty in a metastatic breast cancer patient.

We report the first known case of septic arthritis in the setting of total hip arthroplasty caused by Clostridium cadaveris, a gas-forming anaerobic enteric organism usually considered a non-pathogen. This case occurred in a patient treated with total hip arthroplasty for metastatic breast cancer involving the acetabulum. The patient was managed successfully with debridement, prosthetic retention, and chronic antibiotic suppression.

Surgical treatment for unstable low-grade isthmic spondylolisthesis in adults: a prospective controlled study of posterior instrumented fusion compared with combined anterior-posterior fusion.

Background Context: The surgical treatment for low-grade isthmic spondylolisthesis in adults with intractable lumbar pain is usually spinal fusion. It has been postulated that anterior column reconstruction may be relatively advantageous in those patients with unstable slips.

Purpose: To compare the early and medium term treatment efficacy of two common fusion techniques in isthmic spondylolisthesis.