Exploring the binding behavior mechanism of vitamin B to α-Casein and β-Casein: multi-spectroscopy and molecular dynamic approaches.

The aim of this study was to investigate the behavior interaction of α-Casein-B and β-Casein-B complexes as binary systems through the methods of multiple spectroscopic, zeta potential, calorimetric, and molecular dynamics (MD) simulation. Fluorescence spectroscopy denoted the role ofBas a quencher in both cases of α-Casein and β-Casein fluorescence intensities, which also verifies the existence of interactions. The quenching constants of α-Casein-B and β-Casein-B complexes at 298 K in the first set of binding sites were 2.