Four-Dimensional Data-Independent Acquisition-Based Proteomic Profiling Combined with Transcriptomic Analysis Reveals the Involvement of the Slym1-SlFHY3-CAB3C Module in Regulating Tomato Leaf Color.

In green plants, the chloroplast is responsible for light energy transition and organic assimilation. However, the molecular mechanisms underlying chloroplast development in horticultural crops remain unclear. Here, four-dimensional data-independent acquisition-based proteomic profiling identified 1,727 differentially expressed proteins between "Zhongshu 4" (ZS4) and () leaves, a considerable proportion of which were down-regulated chloroplast proteins.

Optimizing polylactic acid composites: Role of sodium lignosulfonate-modified carbon nanotubes in mechanical and interfacial performance.

In this study, researchers synthesized sodium lignosulfonate (LS) modified multi-walled carbon nanotubes (L-MWCNTs) using a deep eutectic solvent (DES) method and incorporated them into polylactic acid (PLA) composite films using a solvent evaporation method. The nanofiller content ranged between 0.5 % and 1.

Probiotics alleviate chronic ethanol exposure-induced anxiety-like behavior and hippocampal neuroinflammation in male mice through gut microbiota-derived extracellular vesicles.

Background: Probiotics can colonize both the human and animal bodies and consist of active microorganisms that are beneficial to health. The use of probiotics has been shown to alleviate certain neurological diseases and disturbances in gut microbiota resulting from chronic ethanol exposure. Research indicates that probiotics can influence the nervous system via the microbial-gut-brain axis, wherein extracellular vesicles secreted by the gut microbiota play a significant role in this process.

Gut microbiota modulates depressive-like behaviors induced by chronic ethanol exposure through short-chain fatty acids.

Background: Chronic ethanol exposure (CEE) is recognized as an important risk factor for depression, and the gut-brain axis has emerged as a key mechanism underlying chronic ethanol exposure-induced anxiety and depression-like behaviors. Short-chain fatty acids (SCFAs), which are the key metabolites generated by gut microbiota from insoluble dietary fiber, exert protective roles on the central nervous system, including the reduction of neuroinflammation. However, the link between gut microbial disturbances caused by chronic ethanol exposure, production of SCFAs, and anxiety and depression-like behaviors remains unclear.

Mesoporous zinc oxide-based drug delivery system offers an antifungal and immunoregulatory strategy for treating keratitis.

Fungal keratitis is a refractory eye disease that is prone to causing blindness. Fungal virulence and inflammatory responses are two major factors that accelerate the course of fungal keratitis. However, the current antifungal drugs used for treatment usually possess transient residence time on the ocular surface and low bioavailability deficiencies, which limit their therapeutic efficacy.

Extracellular vesicles from Candida albicans modulate immune cells function and play a protective role in fungal keratitis.

Fungal keratitis (FK) is a highly blinding infectious corneal disease caused by pathogenic fungi. Candida albicans (C. albicans) is one of the main pathogens of fungal keratitis.

Biomimetic mercury immobilization by selenium functionalized polyphenylene sulfide fabric.

Highly efficient decontamination of elemental mercury (Hg) remains an enormous challenge for public health and ecosystem protection. The artificial conversion of Hg into mercury chalcogenides could achieve Hg detoxification and close the global mercury cycle. Herein, taking inspiration from the bio-detoxification of mercury, in which selenium preferentially converts mercury from sulfoproteins to HgSe, we propose a biomimetic approach to enhance the conversion of Hg into mercury chalcogenides.

-Derived Extracellular Vesicles Mitigate Fungal Keratitis by Modulating the Immune Cell Function.

Fungal keratitis (FK) is a refractory global disease characterized by a high incidence of blindness and a lack of effective therapeutic options, and (, AF) is one of the most common causative fungi. This study aimed to investigate the role of extracellular vesicles (EVs) from in the immune cell function and their protective role in keratitis in order to explore their therapeutic potential. First, we isolated and characterized the EVs (AF-derived EVs).

Hydrogen-Bonded Organic Framework Derived 2D N, O Co-Doped Carbon Nanobelt with Tunable Pseudocapacitive Contribution for Efficient Capacitive Deionization.

Defect engineering is recognized as an attractive method for modulating the electronic structure and physicochemical characteristics of carbon materials. Exploiting heteroatom-doped porous carbon with copious active sites has attracted great attention for capacitive deionization (CDI). However, traditional methods often rely on the utilization of additional heteroatom sources and strong corrosive activators, suffering from low doping efficiency, insufficient doping level, and potential biotoxicity.

The preparation and therapeutic effects of β-glucan-specific nanobodies and nanobody-natamycin conjugates in fungal keratitis.

Fungal keratitis (FK) is a severe infectious corneal disease. Since traditional eye drops exhibit poor dissolution and high corneal toxicity, the efficacy of current treatments for FK remains limited. It is needed to develop new approaches to control the cornea damage from FK.

Synergistic enhancement of redox pairs and functional groups for the removal of phenolic organic pollutants by activated PMS using silica-composited biochar: Mechanism and environmental toxicity assessment.

In present work, a novel catalyst of cobalt supported on silica-composited biochar (Co@ACFA-BC) derived from fly ash and agricultural waste was synthesized. A series of characterizations confirmed that CoO and Al/Si-O compounds were successfully embedded on the surface of biochar, which triggered superior catalytic activity for PMS activation towards phenol degradation. Particularly, the Co@ACFA-BC/PMS system could completely degrade phenol in the wide pH range, and was almost unaffected by environmental factors including humic acid (HA), HPO, HCO, Cl, and NO.

Effect of fetal lung maturation on the efficacy of acetaminophen for premature infants with patent ductus arteriosus.

The objective of this study was to evaluate the effect of maturing fetal lung on clinical efficacy of acetaminophen in the treatment of premature infants with patent ductus arteriosus (PDA). A total of 441 premature infants admitted to our hospital from May 2020 to May 2021 were recruited, including 152 premature infants receiving fetal lung maturation (13 cases of PDA closure with drug use and 2 cases failed) and 289 cases without maturing fetal lung (17 cases of PDA closure and 8 cases failed). Finally, a total of 30 cases were enrolled in this clinical trial.

Hinokitiol inhibits by interfering with the cell membrane and cell wall.

Hinokitiol (β-thujaplicin) is an important component of the essential oil extracted from , which prevents the decay and decomposition of temple and shrine buildings in Japan. Hinokiol has been shown to have a detrimental effect on various fungi such as and fungi. However how hinokitiol works against () has not been claimed.

Insights into the role of ·OH generated in Fe/CaO/coal slime system for efficient extracellular polymeric substances degradation to improve dewaterability of sewage sludge.

The disposal of massive sewage sludge and coal slime is a problem facing municipalities in China. A hypothesis for the co-disposal of sludge and coal slime is proposed to improve dewaterability by utilizing the beneficial role of coal slime as a filter assist and CaO enhanced system in this research. Results showed that capillary suction time, specific resistance to filtration and water content decreased dramatically from 49.

A Machine Learning-Based Prediction of Diabetes Insipidus in Patients Undergoing Endoscopic Transsphenoidal Surgery for Pituitary Adenoma.

Background: Diabetes insipidus (DI) is a common complication after endoscopic transsphenoidal surgery (TSS) for pituitary adenoma (PA), which affects the quality of life in patients. Therefore, there is a need to develop prediction models of postoperative DI specifically for patients who undergo endoscopic TSS. This study establishes and validates prediction models of DI after endoscopic TSS for patients with PA using machine learning algorithms.

Characterization of neuroendocrine regulation- and metabolism-associated molecular features and prognostic indicators with aid to clinical chemotherapy and immunotherapy of patients with pancreatic cancer.

The worldwide prevalence of pancreatic cancer has been rising in recent decades, and its prognosis has not improved much. The imbalance of substance and energy metabolism in tumour cells is among the primary causes of tumour formation and occurrence, which is often controlled by the neuroendocrine system. We applied Cox and LASSO regression analysis to develop a neuroendocrine regulation- and metabolism-related prognostic risk score model with three genes (GSK3B, IL18 and VEGFA) for pancreatic cancer.

Anticoagulation for mechanical heart valves during pregnancy: A case report and a literature review.

Rationale: Most previous treatment guidelines for pregnant women with mechanical heart valves recommend that low molecular weight heparin (LMWH) should be applied once every 12 hours and only as required to reach peak anti-Xa levels of approximately 1.0 to 1.2 IU/mL, but it is commonly associated with subtherapeutic trough levels, consequently with an inadequate level of anticoagulation.

The microbiota-gut-brain axis in pathogenesis of depression: A narrative review.

The microbiota-gut-brain axis is a bidirectional regulatory pathway between the brain and the gastrointestinal tract, which plays an important role in maintain homeostasis. Gut microbiota could influence the behavior, cognition, stress response and others via the axis. Depression is a complex psychiatric disease, giving rise to heavy social health and economic burden.

Gut microbiota regulates chronic ethanol exposure-induced depressive-like behavior through hippocampal NLRP3-mediated neuroinflammation.

Chronic ethanol exposure (CEE), which can lead to neuroinflammation, is an increasing risk factor for depression disorder, but the underlying mechanism is not clear. Recent observations have revealed the associations among psychiatric disorders, ethanol exposure and alterations of the gut microbiota. Here, we found that CEE induced depressive-like behavior, which could be alleviated by probiotics and transferred from donor to recipient mice by fecal microbiota transplantation (FMT).

Escaping drought: The pectin methylesterase inhibitor gene Slpmei27 can significantly change drought resistance in tomato.

Drought stress will lead to a decrease in tomato yield and poor flavour, yield and quality, resulting in economic losses in agricultural production. Mining the key genes regulating tomato drought resistance is of great significance to improve the drought resistance of tomato plants. The cell wall can directly participate in the plant drought stress response as one of the main components of the cell wall, and the regulation of pectin content in plant drought resistance is still unclear.

Slym1 control the color etiolation of leaves by facilitating the decomposition of chlorophyll in tomato.

Photosynthesis, as an important biological process of plants, produces organic substances for plant growth and development. Although the molecular mechanisms of photosynthesis had been well investigated, the relationship between chlorophyll synthesis and photosynthesis remains largely unknown. The leaf-color mutant was an ideal material for studying photosynthesis and chlorophyll synthesis, which had been seldom investigated in tomato.

Enhanced arsenic removal by reusable hexagonal CeO/FeO nanosheets with exposed (0001) facet.

Arsenite in wastewater has caused increasing concern because of high toxicity and mobility. Iron oxides are widely available and regarded as effective adsorbents for arsenic. However, conventional iron oxides usually are only effective for arsenate (As(V)) adsorption by complexation, but not for As(III) adsorption because of their poor catalytic oxidation activities, which greatly limits arsenic removal efficiency.

Clinical evaluation of the lot-to-lot consistency of an enterovirus 71 vaccine in a commercial-scale phase IV clinical trial.

Objective: To evaluate the immunogenicity, safety and lot-to-lot consistency of an inactivated enterovirus 71 (EV71) vaccine cultured in bioreactors with different specifications after full immunization.

Methods: A randomized, double-blind trial was performed in 3,000 children aged 6 ~ 35 months with six vaccine batches, which were prepared in 40 L and 150 L bioreactors for three consecutive batches respectively. Children were immunized on day 0 and 28, serum samples were collected on day 0 and 56, and neutralizing antibody titers were determined by the microcytopathic method.