Publications by authors named "Fanyuan Sun"
The vast majority of data obtained from sequence analysis of influenza A viruses (IAVs) have revealed that nonstructural 1 (NS1) proteins from H1N1 swine, H3N8 equine, H3N2 avian and the correspondent subtypes from dogs have a conserved four C-terminal amino acid motif when independent cross-species transmission occurs between these species. To test the influence of the C-terminal amino acid motifs of NS1 protein on the replication and virulence of IAVs, we systematically generated 7 recombinants, which carried naturally truncated NS1 proteins, and their last four C-terminal residues were replaced with PEQK and SEQK (for H1N1), EPEV and KPEI (for H3N8) and ESEV and ESEI (for H3N2) IAVs. Another recombinant was generated by removing the C-terminal residues by reverse genetics.
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- H1N1 reassortants from swine have been present in pigs for over ten years and may be linked to more human infections; it's unclear if these viruses gain mutations that make them more dangerous to new hosts.
- Researchers created mouse-adapted versions of a specific EA H1N1 virus isolated from dogs, which showed increased virulence compared to the original virus, with key mutations identified in several viral proteins.
- The study found that mutations in hemagglutinin (HA) genes significantly enhanced virulence and replication, while certain mutations reduced polymerase activity, illustrating how specific genetic changes can impact viral behavior and pathogenicity.
Feline calicivirus (FCV) is a highly infectious pathogen that causes upper respiratory tract disease (URTD), but the enteric FCVs raise concerns regarding their role of an enteric pathogen. In this study, between 2019 and 2020, 101 clinical samples from domestic cats with symptoms of URTD, with or without enteritis, were collected for FCV-specific detection. The FCV-positive rate reached to 42.
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