Oversecretion of CCL3 by Irradiation-Induced Senescent Osteocytes Mediates Bone Homeostasis Imbalance.

Various stressors such as ionizing radiation (IR), chemotherapeutic agents, oxidative stress, and inflammatory responses can trigger the stress-induced premature senescence (SIPS) of cells in the bone microenvironment, including osteocytes. However, little is known about the mechanisms underlying the senescent cellular regulation of the differentiation potential and bone homeostasis. Here, we report a secretory change in senescent osteocytes activated by IR, its subsequent impact on osteogenic and osteoclastic differentiation, and the inflammatory cascade response.

Comparative efficacy of robot-assisted therapy associated with other different interventions on upper limb rehabilitation after stroke: A protocol for a network meta-analysis.

Introduction: Post-stroke movement disorders are common, especially upper limb dysfunction, which seriously affects the physical and mental health of stroke patients. With the continuous development of intelligent technology, robot-assisted therapy has become a research hotspot in the upper limb rehabilitation of stroke patients in recent years. Many scholars have also integrated robot-assisted therapy with other interventions to improve rehabilitation outcomes.

Randomized controlled trial protocol to analyze the effects of rice with different contents of resistant starch on chronic constipation.

The trend of the aging population worldwide is becoming increasingly severe. As people age, constipation becomes increasingly common in older adults, causing varying degrees of physical and psychological harm to them. Dietary intervention is a common nonpharmacological therapy.

Development and validation of a prognostic model for cachexia in postoperative gastric cancer patients with low nutritional risk: a dual-center retrospective cohort study.

Background: Gastric cancer can lead to excessive catabolism in patients. After undergoing gastric surgery, patients may experience additional unintended weight loss, resulting in severe malnutrition and potentially cachexia.

Methods: We selected and incorporated patients from two centers.

Retinol Binding Protein 4 Serves as a Potential Tumor Biomarker and Promotes Malignant Behavior in Gastric Cancer.

Background: Gastric cancer (GC) is a highly phenotypically heterogeneous disease and is caused by a combination of factors. Retinol binding protein 4 (RBP4) is a member of a family of lipid transport proteins that are involved in the transport of substances between cells and play a crucial role in a variety of cancers. However, the expression and role of RBP4 in GC remain unknown.

Barriers and facilitators to feeling safe for inpatients: a model based on a qualitative meta-synthesis.

Objectives: To review and synthesize qualitative research exploring patients' safe experience and construct a model to present barriers and facilitators to feeling safe for inpatients.

Design: A qualitative met-synthesis.

Methods: We conducted a systematic electronic search of articles published in English with no date limitation across five databases (Ovid MEDLINE, EMBASE, Web of Science, CINAIL via EBSCO, and PsyINFO) in May 2023.

An Efficient Approach to the Accurate Prediction of Mutational Effects in Antigen Binding to the MHC1.

The major histocompatibility complex (MHC) can recognize and bind to external peptides to generate effective immune responses by presenting the peptides to T cells. Therefore, understanding the binding modes of peptide-MHC complexes (pMHC) and predicting the binding affinity of pMHCs play a crucial role in the rational design of peptide vaccines. In this study, we employed molecular dynamics (MD) simulations and free energy calculations with an Alanine Scanning with Generalized Born and Interaction Entropy (ASGBIE) method to investigate the protein-peptide interaction between HLA-A*02:01 and the G9 peptide derived from the melanoma antigen gp100.

PLCD3 inhibits apoptosis and promotes proliferation, invasion and migration in gastric cancer.

Gastric cancer (GC) is a heterogeneous disease whose development is accompanied by alterations in a variety of pathogenic genes. The phospholipase C Delta 3 enzyme is a member of the phospholipase C family, which controls substance transport between cells in the body. However, its role in gastric cancer has not been discovered.

C-C Motif Chemokine Ligand 5 (CCL5) Promotes Irradiation-Evoked Osteoclastogenesis.

The imbalance that occurs in bone remodeling induced by irradiation (IR) is the disruption of the balance between bone formation and bone resorption. In this study, primary osteocytes (OCYs) of femoral and tibial origin were cultured and irradiated. It was observed that irradiated OCY showed extensive DNA damage, which led to the initiation of a typical phenotype of cellular senescence, including the secretion of senescence-associated secretory phenotype (SASP), especially the C-C motif chemokine ligand 5 (CCL5).

Clinical and radiological features of a case of primary encephalitis induced by SARS-CoV-2 omicron variant infection: A case report.

Rationale: In 2022, severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) Omicron spread widely around the world. In the context of most literature reporting weakened virulence of the virus, immunocompromised patients who have not been vaccinated should be vigilant for the development of encephalitis following SARS-CoV-2 infection.

Patient Concerns: A 58-year-old male patient with immunodeficiency presented with respiratory and psychiatric symptoms after contracting SARS-CoV-2 Omicron variant.

A multicenter study on the quantification of liver iron concentration in thalassemia patients by means of the MRI T technique.

Objective: To investigate the feasibility and accuracy of quantifying liver iron concentration (LIC) in patients with thalassemia (TM) using 1.5T and 3T T MRI.

Methods: 1.

MolTaut: A Tool for the Rapid Generation of Favorable Tautomer in Aqueous Solution.

Fast and proper treatment of the tautomeric states for drug-like molecules is critical in computer-aided drug discovery since the major tautomer of a molecule determines its pharmacophore features and physical properties. We present MolTaut, a tool for the rapid generation of favorable states of drug-like molecules in water. MolTaut works by enumerating possible tautomeric states with tautomeric transformation rules, ranking tautomers with their relative internal energies and solvation energies calculated by AI-based models, and generating preferred ionization states according to predicted microscopic p.

The state and fate of lake ice thickness in the Northern Hemisphere.

Lake ice thickness (LIT) is important for regional hydroclimate systems, lake ecosystems, and human activities on the ice, and is thought to be highly susceptible to global warming. However, the spatiotemporal variability in LIT is largely unknown due to the difficulty in deriving in situ measurements and the lack of an effective remote sensing platform. Despite intensive development and applications of lake ice models driven by general circulation model output, evaluation of the global LIT is mostly based on assumed "ideal" lakes in each grid cell of the climate forcing data.

Temperature and oxidation-sensitive dioleoylphosphatidylethanolamine liposome stabilized with poly(ethyleneimine)/(phenylthio)acetic acid ion pair.

Temperature and oxidation-sensitive liposomes were prepared by stabilizing dioleoylphosphatidylethanolamine (DOPE) bilayers with the ion pair of poly(ethyleneimine)/(phenylthio)acetic acid (PEI/PTA). An upper critical solution temperature (UCST) behavior was observed when PEI/PTA ion pair was suspended in an aqueous solution. It was observed that the UCST increased with increasing PTA content.

Thermo-sensitive self-assembly of poly(ethylene imine)/(phenylthio) acetic acid ion pair in surfactant solutions.

Poly(ethylene imine)/(phenylthio) acetic acid (PEI/PTA) ion pairs exhibited an upper critical solution temperature (UCST) behavior in an aqueous solution and the UCST was higher as the PTA content was more. The UCST of the ion pair decreased with increasing Brij S100 (BS 100, a nonionic surfactant) concentration but increased with increasing cetylpridinium chloride (CPC, a cationic surfactant) and sodium lauroylsarcosinate (SLS, an anionic surfactant) concentration. TEM microscopy demonstrated BS 100 markedly reduced the size of PEI/PTA ion pair self-assembly (IPSAM) whereas CPC and SLS had little effect on the size and the integrity of IPSAM.

Intelligent Diagnosis and Analysis of Brain Lymphoma Based on DSC Imaging Features.

Currently, DSC has been extensively studied in the diagnosis, differential diagnosis, and prognosis evaluation of brain lymphoma, but it has not obtained a uniform standard. By combining DSC imaging features, this study investigated the imaging features and diagnostic value of several types of tumors such as primary brain lymphoma. At the same time, this study obtained data from brain lymphoma patients by data collection and set up different groups to conduct experimental studies to explore the correlation between IVIMMRI perfusion parameters and DSC perfusion parameters in brain lymphoma.

Multi-echo Dixon and breath-hold T2-corrected multi-echo single-voxel MRS for quantifying hepatic iron overload in rabbits.

Background: Three-dimensional (3D) multi-echo-Dixon (ME-Dixon) and breath-hold T2-corrected multi-echo single-voxel MR spectroscopy (HISTO) can simultaneously quantify liver fat and liver iron. However, their diagnostic efficacy and application scope for quantitative iron in co-existing fatty liver have not been adequately evaluated.

Purpose: To evaluate the accuracy of ME-Dixon and HISTO for quantitative analysis of hepatic iron in rabbits with iron deposition and fatty liver using liver-iron concentration (LIC) as a reference standard.

Combined T2 Mapping and Diffusion Tensor Imaging: A Sensitive Tool to Assess Myofascial Trigger Points in a Rat Model.

Background: Myofascial trigger points (MTrPs) are defined as very small and hypersensitive points in skeletal muscle that are palpable, and produce localized pain on compression. The aim of this study was to explore the feasibility of combining T2 mapping with diffusion tensor imaging (DTI) for assessing MTrPs in a rat model and to investigate properties of the pathophysiological mechanisms.

Methods: Twenty-four Sprague-Dawley rats (model group, n = 14; control group, n = 10) underwent a magnetic resonance imaging (MRI) examination on a 3 T-MRI-scanner with a protocol consisting of T2 mapping and DTI.

Gelatin-loaded cubosomes stabilized with hydrophobically modified quaternized cellulose nanofiber and their pH-dependent release property.

Monoolein cubic phase immobilizing hydrophobically modified gelatin (HmGel) in its water channel was prepared by a melt-hydration method. The cubic phase was micronized into cubosomes by using hydrophobically modified quaternized cellulose nanofiber (HmQCNF) as a stabilizer. The phase transition temperature of the cubic phase was about 68-70 °C.

Liver iron quantification by 3 tesla MRI: calibration on a rabbit model.

Purpose: To determine the feasibility of liver iron quantification by 3 Tesla (T) MRI using a novel iron overload rabbit model.

Materials And Methods: Forty-two rabbits underwent iron dextran loading from 1 to 15 weeks. MRI signal intensity ratio (SIR) was measured using a gradient-echo sequence, and R2(1/T2) measured using an eight-echo spin-echo sequence at 3T.