Prediction of the antigenic regions in eight RhD variants identified by computational biology.

Background And Objectives: Changes in RHD generate variations in protein structure that lead to antigenic variants. The classical model divides them into quantitative (weak and Del) and qualitative (partial D). There are two types of protein antigens: linear and conformational.

Use of computational biology to compare the theoretical tertiary structures of the most common forms of RhCE and RhD.

Background And Objectives: Computational biology analyses the theoretical tertiary structure of proteins and identifies the 'topological' differences between RhD and RhCE. Our aim was to identify the theoretical structural differences between the four isoforms of RhCE and RhD using computational biological tools.

Materials And Methods: Physicochemical profile was determined by hydrophobicity and electrostatic potential analysis.

Analysis of a Novel Mexican Variant of the Gene Associated with β-Thalassemia Using Bioinformatic Tools.

We present a study performed on 54 unrelated subjects, with and without thalassemic features. Two primer pairs were proposed to perform Sanger sequencing of the complete gene. The bioinformatic analysis was performed taking advantage of the availability of free online tools.

[Molecular identification of Hemoglobin D Punjab in cases detected in two families].

Background: Hemoglobin D Punjab is the world most common variant hemoglobin D; in Mexico there are reports of isolated cases. Our goal is to present the clinical and molecular study in two families with HbD Punjab. The objective was to submit molecular diagnosis of two families with Hb D Punjab and clinical features.

[Molecular identification of glucose-6-phosphate dehydrogenase (G6PD) detected in neonatal screening].

Aims: To present the strategy of identifying the molecular variants of G6PD detected in neonatal screening (NS).

Material And Methods: We present a series of incident cases of newborns positive for G6PD deficiency detected in NS. From nuclear DNA with the methodology of real-time PCR we sought molecular G6PD variants: G202A, A376G, T968C and C563T.

Association between genotype and plasma levels of thrombin-activated fibrinolysis inhibitor (TAFI) in the development of preeclampsia.

Introduction: The objective was to evaluate if thrombin-activated fibrinolysis inhibitor (TAFI) polymorphisms (G505A, C1040T, and G-438A), and TAFIa plasma levels are associated with preeclampsia.

Materials And Methods: In a case-control study design, we evaluated preeclampsia patients and women with uncomplicated pregnancies. The TAFI polymorphisms were determined by real-time PCR method, and TAFIa plasma levels were established with a chromogenic assay.

[RH hybrid box identification in subjects with Rh negative phenotype from Mexico's Valley].

Background: The prevalence of the RhD and RhCE gene alleles is related to the ethnic mixture. The aim of this report is to describe the predominant molecular mechanisms in RhD negative subjects residents from Mexico's valley according to the phenotype of RhCE.

Methods: Blood samples from RhD negative women and men were studied.

Association of HFE mutations (C282Y and H63D) with iron overload in blood donors from Mexico City.

Background And Objective: Iron overload has been associated with HFE mutations (C282Y and H63D). We investigated the association between these mutations and high serum ferritin in a sample of healthy adult men.

Design And Methods: We enrolled unrelated blood donors from three hospitals in Mexico City in a crosssectional study.

Changes of coagulation inhibitors and fibrinolysis system in newborn infants with transitory neonatal cholestasis.

Introduction: The hemostatic system in newborn is a dynamic evolving process health-status dependent.

Objective: To explore the changes in coagulation inhibitors and fibrinolytic system in newborn with neonatal cholestasis, according liver damage intensity.

Methods: In a cross-sectional design, we studied fibrinolysis and coagulation inhibitor proteins, and serum ferritin (SF) in patients with neonatal cholestasis.