Using diagnostic data from veterinary diagnostic laboratories to unravel macroepidemiological aspects of porcine circoviruses 2 and 3 in the United States from 2002-2023.

Porcine circoviruses (PCVs), including porcine circovirus 2 (PCV2) and porcine circovirus 3 (PCV3), have been associated with clinical syndromes in swine, resulting in significant economic losses. To better understand the epidemiology and clinical relevance of PCV2 and PCV3, this study analyzed a dataset comprising diagnostic data from six veterinary diagnostic laboratories (VDLs) in the United States of America. The data comprised of polymerase chain reaction (PCR) test results, sample type, and age group for PCV2 and PCV3 submissions from 2002-2023.

Detection and disease diagnosis trends (2017-2022) for Streptococcus suis, Glaesserella parasuis, Mycoplasma hyorhinis, Actinobacillus suis and Mycoplasma hyosynoviae at Iowa State University Veterinary Diagnostic Laboratory.

Background: Accurate measurement of disease associated with endemic bacterial agents in pig populations is challenging due to their commensal ecology, the lack of disease-specific antemortem diagnostic tests, and the polymicrobial nature of swine diagnostic cases. The main objective of this retrospective study was to estimate temporal patterns of agent detection and disease diagnosis for five endemic bacteria that can cause systemic disease in porcine tissue specimens submitted to the Iowa State University Veterinary Diagnostic Laboratory (ISU VDL) from 2017 to 2022. The study also explored the diagnostic value of specific tissue specimens for disease diagnosis, estimated the frequency of polymicrobial diagnosis, and evaluated the association between phase of pig production and disease diagnosis.

A diagnostic approach to confirm Mycoplasma hyopneumoniae "Day zero" for pathogen eradication.

Breeding herds in the US are trending toward eradication of Mycoplasma hyopneumoniae (M. hyopneumoniae) due to the positive impact on welfare and downstream production. In an eradication program, "Day 0″ is the time point when the last replacement gilts to enter the herd were exposed to M.

Duration of Mycoplasma hyopneumoniae detection in pigs following purposeful aerosol exposure.

Swine disease elimination programs for Mycoplasma hyopneumoniae are commonly applied in the North American swine industry and may include the aerosolization of medium containing lung tissue to achieve population exposure prior to start. Field data has indicated M. hyopneumoniae PCR detection in pigs beyond 240 days post-herd closure (dphc; planned end of an elimination program) and is thought to contribute to disease elimination programs' failure.

Pandemic news information uncertainty-News dynamics mirror differential response strategies to COVID-19.

National differences in uncertainty, inequality, and trust have been accentuated by COVID-19. There are indications that the pandemic has impacted societies characterized by high uncertainty, inequality, and low trust harder than societies characterized by low uncertainty, equality, and high trust. This study investigates differential response strategies to COVID-19 as reflected in news media of two otherwise similar low uncertainty societies: Denmark and Sweden.

The effect of gilt flow management during acclimation on Mycoplasma hyopneumoniae detection.

The objective of this study was to characterize the Mycoplasma hyopneumoniae (M. hyopneumoniae) detection and seroconversion patterns in recently acclimated gilts to be introduced to endemically infected farms using different types of replacement management. Three gilt developing units (GDUs) belonging to sow farms were included in this investigation: two farms managed gilts in continuous flow, and one farm managed gilts all-in/all-out.

Evaluation of dam parity and internal biosecurity practices in influenza infections in piglets prior to weaning.

Influenza is an important respiratory disease of pigs and humans. Controlling influenza in pigs is challenging due to the substantial genetic diversity of influenza A virus (IAV). In this study, we assessed the impact of internal biosecurity practices directed at limiting exposure of piglets to IAV before weaning; evaluated the association of sow parity with IAV prevalence in piglets and the levels of maternally derived antibodies (MDA), and documented the frequency of detection of IAV on farmworkers' hands and the instruments used when handling pigs.

Hair cortisol as a biomarker of chronic stress in preadolescents: influence of school context and bullying.

Bullying has been identified as the most common form of aggression and a major source of stress among children and adolescents. The main objective of this study was to analyze the association that school context in general and bullying in particular might have with hair cortisol concentration (HCC), examining the effect of executive function and sex on this association. The study included 659 11-year-old preadolescents from the cohorts of Gipuzkoa and Sabadell of the INMA (INfancia y Medio Ambiente-Children and Environment) project.

Species-specific efficiency in PM removal by urban trees: From leaf measurements to improved modeling estimates.

The growing population in cities is causing a deterioration of air quality due to the emission of pollutants, causing serious health impacts. Trees and urban forests can contribute through the interception and removal of air pollutants such as particulate matter (PM). The dry deposition of PM by vegetation depends on air pollutant concentration, meteorological conditions, and specific leaf traits.

Do prepubertal hormones, 2D:4D index and psychosocial context jointly explain 11-year-old preadolescents' involvement in bullying?

Background: Bullying is a type of aggressive behavior that occurs repeatedly and intentionally in school environments and where there is a power imbalance. The main objective of this study was to analyze the association that hormones and the psychosocial context jointly have with bullying behavior.

Method: Participants were 302 11-year-old preadolescents from the Gipuzkoan cohort of the INMA Project.

Cough associated with the detection of Mycoplasma hyopneumoniae DNA in clinical and environmental specimens under controlled conditions.

Background: The association of cough with Mycoplasma hyopneumoniae (MHP) DNA detection in specimens was evaluated under conditions in which the MHP status of inoculated and contact-infected pen mates was closely monitored for 59 days post-inoculation (DPI).

Methods: Seven-week-old pigs (n = 39) were allocated to five rooms (with one pen). Rooms contained 9 pigs each, with 1, 3, 6, or 9 MHP-inoculated pigs, respectively, except Room 5 (three sham-inoculated pigs).

The achievement of Water Framework Directive goals through the restoration of vegetation in agricultural canals.

Decreasing nitrate concentrations is one of the most relevant Water Framework Directive (WFD) goals, which today is still unreached in several European countries. Vegetated canals have been recognized as effective filters to mitigate nitrate pollution, although rarely included in restoration programs aimed at improving water quality in agricultural watersheds. The Po di Volano basin (713 km, Northern Italy) is a deltaic territory crossed by an extensive network of agricultural canals (~1300 km).

Plastic (PET) vs bioplastic (PLA) or refillable aluminium bottles - What is the most sustainable choice for drinking water? A life-cycle (LCA) analysis.

Nowadays, the most important tool to evaluate the environmental impact of both petro-plastics and bioplastics is the life cycle analysis (LCA). LCA determines the overall impact on the environment by defining, calculation and analyzing all the input and output directly related to production, utilization, and disposal of a product or a process. In this work, a LCA (cradle to grave) of bottles for drinking water was developed on three scenarios: polyethylene terephthalate (PET) bottles, as conventional packaging material for outdoor drinking water, polylactic acid (PLA) bottles, as alternative and innovative biodegradable packaging and aluminum bottle, as reusable and almost infinitely refilling packaging.

Pooled-sample testing for detection of Mycoplasma hyopneumoniae during late experimental infection as a diagnostic tool for a herd eradication program.

Early and accurate detection of Mycoplasma hyopneumoniae infection in live pigs is a critical component to measure the success of disease eradication strategies. However, the imperfect sensitivity of in vivo diagnostic tools, change in sensitivity over the course of infection, and expected low prevalence level at the end of an eradication program create a challenging diagnostic scenario. Here, the individual and pool sensitivities for detection of M.

Mycoplasma hyopneumoniae Surveillance in Pig Populations: Establishing Sampling Guidelines for Detection in Growing Pigs.

Antemortem detection of infection in swine production systems has relied on antibody testing, but the availability of tests based on DNA detection and novel diagnostic specimens, e.g., tracheal swabs and oral fluids, has the potential to improve surveillance.

Relationships among Fecal, Air, Oral, and Tracheal Microbial Communities in Pigs in a Respiratory Infection Disease Model.

The association of the lower respiratory tract microbiome in pigs with that of other tissues and environment is still unclear. This study aimed to describe the microbiome of tracheal and oral fluids, air, and feces in the late stage of infection in pigs, and assess the association between the tracheal microbiome and those from air, feces, and oral fluids. Tracheal fluids (n = 73), feces (n = 71), oropharyngeal fluids (n = 8), and air (n = 12) were collected in seeder pigs (inoculated with ) and contact pigs (113 days post exposure to seeder pigs).

Effect of multiple vaccinations on transmission and degree of Mycoplasma hyopneumoniae infection in gilts.

Mycoplasma hyopneumoniae (M. hyopneumoniae) infections continue to result in significant respiratory challenges in the swine industry worldwide. Vaccination for M.

Perfluorooctanoic acid-induced cellular and subcellular alterations in fish hepatocytes.

Liver perfluorooctanoic acid (PFOA) pathophysiology and related morphofunction disturbances were studied in common carp at the cellular and subcellular level and with box-counting fractal analysis of ultrathin sections to assess the effect of PFOA exposure on hepatocyte structure complexity and heterogeneity. Three experimental groups were investigated: unexposed; low exposure (200 ng L PFOA); high exposure (2 mg L PFOA). PFOA-exposed cells showed differences from controls at both tested concentrations, manifested mainly as cloudy swelling and reversible vacuolar degeneration.

genetic variability within swine production flows.

The aim of this study was to assess the genetic variability of within various swine production flows. Four positive production flows, composed of 4 production stages, were selected for this study. Laryngeal and/or bronchial swabs were collected from each production stage within a flow, for a period of 4 months up to 3 years.

Risk and Protective Factors for Bullying at 11 Years of Age in a Spanish Birth Cohort Study.

(1) Background: Bullying affects a large number of children worldwide. This study has two objectives, to provide data on the prevalence of bullying in Spain, and to identify risk and protective factors associated with bullying. (2) Methods: Participants were 858 eleven-year-old children.

Temporal dynamics of species associations in the parasite community of European eels, , from a coastal lagoon.

The resilience of biological communities is of central importance in ecology, but is difficult to investigate in nature. Parasite communities in individual hosts provide good model systems, as they allow a level of replication usually not possible with free-living communities. Here, using temporal data (2005-2017) on the communities of endohelminth parasites in European eels, , from brackish-water lagoons in Italy, we test the resilience of interspecific associations to changes in the abundance of some parasite species and the disappearance of others.

Maternal Iodine Status During Pregnancy Is Not Consistently Associated with Attention-Deficit Hyperactivity Disorder or Autistic Traits in Children.

Background: Severe iodine deficiency during pregnancy can cause intellectual disability, presumably through inadequate placental transfer of maternal thyroid hormone to the fetus. The association between mild-to-moderate iodine deficiency and child neurodevelopmental problems is not well understood.

Objectives: We investigated the association of maternal iodine status during pregnancy with child attention-deficit hyperactivity disorder (ADHD) and autistic traits.

The role of species introduction in modifying the functional diversity of native communities.

Although one of the most evident effects of biological invasions is the loss of native taxonomic diversity, contrasting views exist on the consequences of biological invasions on native functional diversity. We investigated this topic using Mediterranean stream, river and canal fish communities as a test case, at 3734 sites in Italy, and distinguishing between exotic and translocated species invasion in three different faunal districts. Our results clearly confirmed that introduced species were widespread and in many cases the invasion was severe (130 communities were completely composed by introduced species).