Preparation of Bunyavirus-Infected Cells for Electron Cryo-Tomography.

Cellular electron cryo-tomography (cryoET) produces high-resolution three-dimensional images of subcellular structures in a near-native frozen-hydrated state. These three-dimensional images are obtained by recording a series of two-dimensional tilt images on a transmission electron cryo-microscope that are subsequently back-projected to form a tomogram. Key to a successful experiment is however a high-quality sample.

Thermodynamic study of crown ether-lithium/magnesium complexes based on benz-1,4-dioxane and its homologues.

The synthesis and characterization of benz-1,4-dioxane crown ethers (CEs) and some of its homologues are described and analyzed. The effect of added C-atom within the CE ring (increasing the hydrophobicity of the CE ring by increasing the number of CH-units) on the Li and Mg complexation within a liquid-liquid extraction (LLE) is investigated and thermodynamically analyzed. The complex stability constant , the change of entropy Δ and enthalpy Δ, and the Gibbs energy Δ are determined.