Effects of dietary yeast culture on health status in digestive tract of juvenile Pacific white shrimp .

A feeding trial was conducted to investigate the effects of dietary yeast culture (YC) on health status in digestive tract of juvenile Pacific white shrimp . Shrimps (initial weight: 3.33 ± 0.

An Uncharacterized Major Facilitator Superfamily Transporter From Exhibits Dual Functions as a Na(Li, K)/H Antiporter and a Multidrug Efflux Pump.

Within major facilitator superfamily (MFS), up to 27 unknown major facilitator families and many members of 60 well-characterized families have been functionally unknown as yet, due to their sharing no or significantly low sequence identity with characterized MFS members. Here we present the first report on the characterization of one functionally unknown MFS transporter designated MdrP with the accession version No. ANU18183.

Characterization of a Functionally Unknown Arginine-Aspartate-Aspartate Family Protein From and Functional Analysis of Its Conserved Arginine/Aspartate Residues.

Arginine-aspartate-aspartate (RDD) family, representing a category of transmembrane proteins containing one highly conserved arginine and two highly conserved aspartates, has been functionally uncharacterized as yet. Here we present the characterization of a member of this family designated RDD from the moderate halophile NEAU-ST10-40 and report for the first time that RDD should function as a novel Na(Li, K)/H antiporter. It's more interesting whether the highly conserved arginine/aspartate residues among the whole family or between RDD and its selected homologs are related to the protein function.

Characterization of a novel two-component Na(Li, K)/H antiporter from Halomonas zhaodongensis.

In this study, genomic DNA was screened for novel Na/H antiporter genes from Halomonas zhaodongensis by selection in Escherichia coli KNabc lacking three major Na/H antiporters. Co-expression of two genes designated umpAB, encoding paired homologous unknown membrane proteins belonging to DUF1538 (domain of unknown function with No. 1538) family, were found to confer E.

A novel NhaD-type Na/H antiporter from the moderate halophile and alkaliphile Halomonas alkaliphila.

In this study, a NhaD-type Na/H antiporter gene designated Ha-nhaD was obtained by selection of genomic DNA from the moderate halophile and alkaliphile Halomonas alkaliphila in Escherichia coli KNabc lacking 3 major Na/H antiporters. The presence of Ha-NhaD conferred tolerance of E. coli KNabc to NaCl up to 0.