Co-existence of :: fusion and variant in a pediatric case with acute myeloid leukemia: a case report and literature review.

The lysine(K)-specific methyltransferase 2A gene (), previously known as mixed lineage leukemia (), frequently rearranged in acute leukemia, belongs to one of the most promiscuous genes and has been found fused to more than 80 different partners. :: fusion is a relatively uncommon rearrangement observed in pediatric acute myeloid leukemia (AML) patients, some of which may harbor other mutations. We herein report a case of AML-M4-infant with :: fusion and variant.

Molecular and clinical characterization of human adenovirus associated with acute respiratory tract infection in hospitalized children.

Background: Human adenovirus (HAdV) is a common pathogen in children that can cause acute respiratory tract infection (ARTI), but the molecular epidemiological and clinical information relating to HAdV among hospitalized children with ARTI are few reported in China.

Objectives: To evaluate the epidemiological, clinical, and molecular characteristics of HAdV infections among hospitalized children with ARTI in Hebei, Northern China from June 2017 to May 2018.

Study Design: A 12-month longitudinal, retrospective study on HAdV, typed by nested polymerase chain reaction targeting the hexon gene's hypervariable region (typing was merely performed by sequencing of the hexon neutralization epitope and thus genotypes could not be identified unequivocally), associated with ARTI was performed.

Highly specific monitoring and imaging of endogenous and exogenous cysteine in living cells.

Cys is one of the important biothiols and its abnormal concentration may pose a threat to human health. Therefore, the monitoring of Cys in organisms is of great significance. GSH and Hcy, as the other two biothiols, have similar chemical structures and active sites to Cys.

A rapid and sensitive recombinase aided amplification assay to detect hepatitis B virus without DNA extraction.

Background: Hepatitis B virus (HBV) infection is the major public health problem worldwide. In clinical practice, serological and molecular assays are the most commonly used diagnostic methods to detect HBV infection in clinical practices.

Methods: Here we present a rapid and sensitive recombinase aided amplification assay (RAA) to detect HBV at 39.

A coumarin-based colorimetric fluorescent probe for rapid response and highly sensitive detection of hydrogen sulfide in living cells.

Hydrogen sulfide (HS) plays a vital role in numerous biological processes in living organisms. To better understand its functions, a fluorescent probe to fast and sensitively detect HS is imminently needed. Keep this in mind, we reasonably designed probe DHC for detecting HS based on α, β-unsaturated ethanoylcoumarin fluorophore.

Dual-Site and Dual-Excitation Fluorescent Probe That Can Be Tuned for Discriminative Detection of Cysteine, Homocystein, and Thiophenols.

Thiols play a vital role in both the physiological process and organic synthesis field, including aliphatic thiols (e.g., Cys, Hcy, and GSH) and thiophenols.

A case control study on the prevalence of enterovirus in children samples and its association with diarrhea.

Some serotypes of enterovirus (EV) may lead to transient and symptomatic gastrointestinal infections while others are commensal residents of the human gut. To determine whether certain EV types are more often associated with diarrhea, we conducted a preliminary study on the prevalence of EV serotypes and common diarrhea viruses in fecal samples of diarrhea children and healthy controls. EV was tested with one step nest polymerase chain reaction and typed by direct sequencing while common causative diarrhea viruses rotavirus (RV), norovirus (NoV), adenovirus (AdV), bocavirus (HBoV), and astrovirus (AstV) were screened with multiplex PCR assays.

A family of mixed-lanthanide metal-organic framework thermometers in a wide temperature range.

By choosing 2-pyridin-4-yl-4,5-imidazoledicarboxylic acid (H3PIDC) as the first ligand and sodium oxalate (OX) as the ancillary ligand, a series of mixed-lanthanide metal-organic frameworks (M'LnMOFs) [Tb1-xEux(HPIDC)(ox)1/2H2O]·3H2O (x = 0 1, 0.01 2a, 0.03 2b, 0.

Adenovirus associated with acute diarrhea: a case-control study.

Background: Diarrhea is a major source of morbidity and mortality among young children in low-income and middle-income countries. Human adenoviruses (HAdV), particular HAdV species F (40, 41) has been recognized as important causal pathogens, however limited data exist on molecular epidemiology of other HAdV associated with acute gastroenteritis.

Methods: In the present preliminary study, we performed a case-control study involving 273 children who presented diarrheal disease and 361 healthy children matched control in Children's hospital of Hebei Province (China) to investigate the relationship between non-enteric HAdV and diarrhea.

[Case-control study on the association between four single nucleotide polymorphisms in folate metabolism way and the risk of congenital heart disease].

Objective: To investigate the association between single nucleotide polymorphisms( SNPs) of 5, 10-methylenetetrahydrofolate reductase( MTHFR) C677T, A1298C, methionine synthase( MS) A2756G, methionine synthase reductase( MTRR) A66G and the risk of congenital heart disease( CHD).

Methods: By restricting the corresponding conditions, a case-control study was performed. We collected 200 congenital heart disease children as case group and 200 normal children as control group admitted to the Department of cardiac surgery, Children 's Hospital of Hebei Province from January 2016 to April 2017.

A probe directed recombinase amplification assay for detection of MTHFR A1298C polymorphism associated with congenital heart disease.

Single nucleotide polymorphisms (SNPs) play an important role in susceptibility to complex diseases, treatment efficacy and adverse drug responses. Conventional methods to detect SNPs are usually based on PCR or DNA sequencing, which are typically time-consuming and require sophisticated equipment. In this proof-of-concept study, a probe-directed recombinase amplification (PDRA) assay was developed to detect the A1298C polymorphism of 5,10-methylenetetrahydrofolate reductase (MTHFR).

A multifunctional Eu-CP as a recyclable luminescent probe for the highly sensitive detection of Fe/Fe, CrO, and nitroaromatic explosives.

A novel 2D lanthanide coordination polymer {Eu-CP (1)} based on a 3-bis(3-carboxyphenyl)imidazolium (L) ligand was successfully assembled through a solvothermal method. Luminescence tests indicate that 1 has excellent selectivity and sensitivity to detect Fe3+/Fe2+, Cr2O72-, and a series of nitroaromatic explosives. Interestingly, 1 can effectively distinguish CrVI anions (CrO42-/Cr2O72-), which is difficult for most of the reported CPs.

A triplex quantitative real-time PCR assay for differential detection of human adenovirus serotypes 2, 3 and 7.

Background: Human adenovirus (HAdV) serotypes 2, 3 and 7 are more prevalent than other serotypes and have been associated with severe pneumonia in pediatric children. Molecular typing of HAdV is not routinely performed in clinical diagnostic laboratories as it is time-consuming and labor-intensive.

Methods: In the present study, we developed a triplex quantitative real-time PCR assay (tq-PCR) in a single closed tube for differential detection and quantitative analysis of HAdV serotypes 2, 3 and 7.

A novel and highly sensitive real-time nested RT-PCR assay in a single closed tube for detection of enterovirus.

The sensitivity of qRT-PCR assay is not adequate for the detection of the samples with lower viral load, particularly in the cerebrospinal fluid (CSF) of patients. Here, we present the development of a highly sensitive real-time nested RT-PCR (RTN RT-PCR) assay in a single closed tube for detection of human enterovirus (HEV). The clinical performance of both RTN RT-PCR and qRT-PCR was also tested and compared using 140 CSF and fecal specimens.

Microsolvation effects on the reactivity of oxy-nucleophiles: the case of gas-phase S2 reactions of YO(CHOH) towards CHCl.

The modified G4(MP2) method was applied to explore microsolvation effects on the reactivity of four solvated normal oxy-nucleophiles YO(CHOH) (Y = CH, CH, FCH, ClCH), and five α-oxy-nucleophiles YO(CHOH) (Y = HO, CHO, F, Cl, Br), in gas-phase S2 reactions towards the substrate CHCl. Based on a Brønsted-type plot, our calculations reveal that the overall activation barriers of five microsolvated α-oxy-nucleophiles are obviously smaller than the prediction from the correlation line constructed by four normal microsolvated ones to different degrees, and clearly demonstrate the existence of an α-effect in the presence of one or two methanol molecule(s). Moreover, it was found that the α-effect of the mono-methanol microsolvated α-nucleophile is stronger than that of the monohydrated α-nucleophile.