Microbiological safety assessment of restaurants and HACCP-certified kitchens in hotels: A study in eastern China.

The Hazard Analysis and Critical Control Point (HACCP) system plays a crucial role in ensuring food safety within food service establishments, effectively reducing the risk of foodborne diseases. This study focused on assessing the risk of microbe contamination in poultry-based cook-served food during meal preparation in four restaurants and five selected HACCP-certified hotels in eastern China. We examined samples collected from 26 poultry-based cooked dishes, 248 food contact surfaces, 252 non-food contact surfaces, and 121 hand swabs.

Correction: Liu et al. The Discovery of Novel Agents against by Targeting Sortase A: A Combination of Virtual Screening and Experimental Validation. 2024, , 58.

In the original publication [...

The Discovery of Novel Agents against by Targeting Sortase A: A Combination of Virtual Screening and Experimental Validation.

, commonly known as "superbugs", is a highly pathogenic bacterium that poses a serious threat to human health. There is an urgent need to replace traditional antibiotics with novel drugs to combat . Sortase A (SrtA) is a crucial transpeptidase involved in the adhesion process of .

Evaluation of Hygiene Practice for Reducing Contamination on Cutting Boards and Risks Associated with Chicken Handling in Kitchen Environment.

Cutting boards can serve as potential carriers for the cross-contamination of pathogens from chicken to other surfaces. This study aimed to assess chefs' handling practices of cutting boards across five provinces in China and identify the key factors contributing to unsafe cutting board usage, including cleaning methods and handling practices. Handling practices associated with cutting boards were examined through a web-based survey (N = 154), while kitchen environment tests were conducted to investigate the splashing or survival of , inoculated in chicken or on cutting boards, to mimic the practices of chefs.

Feeding Malic Acid to Chickens at Slaughter Age Improves Microbial Safety with Regard to .

This study supplied malic acid-supplemented drinking water to flocks that were naturally -positive and assessed the effect of feeding malic acid to chickens on reduction and poultry production. In Experiment 1, chickens were provided with malic acid-supplemented drinking water for three weeks. The contamination loads of were decreased by 0.

An Investigation into the Critical Factors Influencing the Spread of during Chicken Handling in Commercial Kitchens in China.

Campylobacteriosis is the most common cause of bacterial gastroenteritis worldwide. Consumption of chicken meat is considered the main route for human infection with . This study aimed to determine the critical factors for cross-contamination in Chinese commercial kitchens during chicken handling.

Characterization and Prevalence of spp. From Broiler Chicken Rearing Period to the Slaughtering Process in Eastern China.

is one of the most important foodborne pathogens worldwide, and poultry is regarded as the main reservoir of . The contamination of in broiler chickens at the farm level is closely related to the transmission of in the poultry production chain. This study identified 464 isolates from 1,534 samples from broiler rearing period and slaughtering process including 233 isolates and 231 isolates.

Investigating the Role of FlhF Identifies Novel Interactions With Genes Involved in Flagellar Synthesis in .

FlhF is a key protein required for complete flagellar synthesis, and its deletion results in the complete absence of a flagella and thus motility in . However, the specific mechanism still remains unknown. In this study, RNA-Seq, EMSAs, ChIP-qPCR and β-Galactosidase assays were performed to elucidate the novel interactions between FlhF and genes involved in flagellar synthesis.

Insights into the impact of flhF inactivation on Campylobacter jejuni colonization of chick and mice gut.

Background: Campylobacter jejuni (C. jejuni) is a leading cause of foodborne gastroenteritis worldwide. This bacterium lacks many of the classical virulence factors, and flagellum-associated persistent colonization has been shown to be crucial for its pathogenesis.

Could FlhF be a key element that controls Campylobacter jejuni flagella biosynthesis in the initial assembly stage?

The disordered arrangement of flagella biosynthetic genes, combined with a simplified regulatory mechanism, has made elucidating the process of Campylobacter jejuni flagellation difficult. FlhF is a recently identified element that controls the assembly of the flagella, although its function mechanism and regulatory preference are not well defined at present. In this study, we found that inactivation of FlhF caused the transcription of most flagella genes down-regulated.

cj0371: A Novel Virulence-Associated Gene of Campylobacter jejuni.

Campylobacter jejuni is the major cause of human bacterial diarrhea worldwide. Its pathogenic mechanism remains poorly understood. cj0371 is a novel gene that was uncovered using immunoscreening.

Insights into Campylobacter jejuni colonization and enteritis using a novel infant rabbit model.

A lack of relevant disease models for Campylobacter jejuni has long been an obstacle to research into this common enteric pathogen. Here we used an infant rabbit to study C. jejuni infection, which enables us to define several previously unknown but key features of the organism.

[Preparation and characterization of monoclonal antibodies against cytolethal distending toxin protein of Campylobacter jejuni].

Objective: To express Campylobacter jejuni cytolethal distending toxin B protein (CdtB) in a prokaryote to prepare monoclonal antibodies (mAbs) against the protein, and to study their antitoxic effects.

Methods: The C. jejuni cdtB gene was amplified and inserted into the expression plasmids pET-30a( + ) and pGEX-6p-1.

Use of in vivo-induced antigen technology to identify in vivo-expressed genes of Campylobacter jejuni during human infection.

Campylobacter jejuni is a prevalent foodborne pathogen worldwide. Human infection by C. jejuni primarily arises from contaminated poultry meats.

[Preparation and characterization of monoclonal antibodies against FlhF protein of Campylobacter jejuni].

Objective: To develop a prokaryotic expression system for Campylobacrer jejuni FlhF protein and prepare monoclonal antibodies(mAb) against this protein.

Methods: The C. jejuni flhF gene was amplified and inserted into the expression plasmids, pET-30a(+) and pGEX-6p-1.

A novel immunoproteomics method for identifying in vivo-induced Campylobacter jejuni antigens using pre-adsorbed sera from infected patients.

Background: Campylobacter jejuni is an important food-borne and zoonotic pathogen with a worldwide distribution. Humans and chickens are hosts of this pathogen. At present, there is no ideal vaccine for controlling human campylobacteriosis or the carriage of C.