Publications by authors named "Fangyun Qin"
Article Synopsis
- Reverse transcription is vital for HIV-1 replication, and the cellular protein eEF1A interacts directly with the virus's reverse transcriptase (RT), which is essential for the last stages of reverse transcription.
- Experiments using biolayer interferometry and co-immunoprecipation demonstrated a strong binding affinity between eEF1A and RT, highlighting eEF1A as a key cellular protein that binds to RT.
- A specific mutation in the RT, W252A, weakened the eEF1A-RT interaction and hindered HIV-1 replication, while the compound didemnin B, which targets eEF1A, effectively inhibited HIV-1 reverse transcription, confirming the significance of their interaction in the virus
Eukaryotic elongation factor 1 complex subunits are critical HIV-1 reverse transcription cofactors.
Proc Natl Acad Sci U S A
June 2012
Cellular proteins have been implicated as important for HIV-1 reverse transcription, but whether any are reverse transcription complex (RTC) cofactors or affect reverse transcription indirectly is unclear. Here we used protein fractionation combined with an endogenous reverse transcription assay to identify cellular proteins that stimulated late steps of reverse transcription in vitro. We identified 25 cellular proteins in an active protein fraction, and here we show that the eEF1A and eEF1G subunits of eukaryotic elongation factor 1 (eEF1) are important components of the HIV-1 RTC.
View Article and Find Full Text PDF[Study on the molecular epidemiology of Streptococcus suis type 2 from healthy pigs in Guangxi].
Zhonghua Liu Xing Bing Xue Za Zhi
June 2007
Objective: In order to investigate the positive rate of streptococcus suis type 2 and the genes of their suilysin (sly), extracellular protein (epf) and muramidasa-released protein ( mrp) and to understand the antibiotic susceptibility of S. suis type 2.
Methods: S.