Impact of the sterol O-acyltransferase gene (Cmare2) on morphology and stability of the mushroom Cordyceps militaris.

Strain degeneration in mushroom cultivation can lead to significant commercial losses, yet the genetic factors remain elusive. This study identified the sterol O-acyltransferase gene, Cmare2, which is associated with colony sectorization in Cordyceps militaris, using a T-DNA insertion-mutant library. CmARE2 is conserved across Ascomycota and Basidiomycota.

Proteomic Analysis Reveals Differentially Expressed Proteins in Cordyceps militaris Cultured with Different Media.

Cordyceps militaris is rich in high quality protein, which is an excellent protein supplement. In this study, proteins were extracted from C. militaris cultured with tussah pupa and C.

ARTP mutagenesis of Aureobasidium pullulans RM1603 for high pullulan production and transcriptome analysis of mutants.

Pullulan is a microbial exopolysaccharide produced by Aureobasidium spp. with excellent physical and chemical properties, resulting in great application value. In this study, a novel strain RM1603 of Aureobasidium pullulans with high pullulan production of 51.

Characterization, Antioxidant Capacity and Protective Effect of Peptides from Cultivated with Tussah Pupa on Oxidative Injured HepG2 Cells.

The antioxidant capacity and protective effect of peptides from protein hydrolysate of cultivated with tussah pupa (ECPs) on HO-injured HepG2 cells were studied. Results indicated ECP1 (<3 kDa) presented the strongest antioxidant activity compared with other molecular weight peptides. Pretreated with ECPs observably enhanced survival rates and reduced apoptosis rates of HepG2 cells.

Characteristic analysis and fermentation optimization of a novel Aureobasidium pullulans RM1603 with high pullulan yield.

A high-yielding microbial polysaccharide-producing strain, named RM1603, was isolated from rhizosphere soil and identified by morphological and phylogenetic analysis. The extracellular polysaccharides (EPS) were identified by thin-layer chromatography and infrared spectroscopy. The fermentation conditions were optimized by single factor experiments in shake flasks and a 5-L fermentor.

Systemic metabolic engineering of Enterobacter aerogenes for efficient 2,3-butanediol production.

2,3-Butanediol (2,3-BDO) is an important gateway molecule for many chemical derivatives. Currently, microbial production is gradually being recognized as a green and sustainable alternative to petrochemical synthesis, but the titer, yield, and productivity of microbial 2,3-BDO remain suboptimal. Here, we used systemic metabolic engineering strategies to debottleneck the 2,3-BDO production in Enterobacter aerogenes.

Optimization of hydrogen production in Enterobacter aerogenes by Complex I peripheral fragments destruction and maeA overexpression.

As a concentrated energy source with high added value, hydrogen has great development prospects, with special emphasis on sustainable microbial production as a replacement for traditional fossil fuels. In this study, λ-Red recombination was used to alter the activity of Complex I by single and combined knockout of nuoE, nuoF and nuoG. In addition, the conversion of malic to pyruvic acid was promoted by overexpressing the maeA gene, which could increase the content of NADH and formic acid in the bacterial cells.

Characterization and bioactive component analysis of filamentous bacterium XJ-16.

Actinomycetes, which can produce a variety of bioactive compounds in the metabolic process, is one of the important sources of novel drugs, enzymes, anti-tumor drugs and enzyme inhibitors. It has been the focus of researchers to find and develop with special characters. Strain XJ-16 is a blue alkali-resistant filamentous bacterium with high antimicrobial activity isolated from saline-alkali land of Xinjiang.

Integrated strategy of CRISPR-Cas9 gene editing and small RNA RhyB regulation in : A novel protocol for improving biohydrogen production.

Dark fermentation is considered as one of the most practical biological hydrogen production methods. However, current productivity and yield are still not economically viable for industrial applications. This biological process must be improved through multiple strategies, of which screening for more effective microbial strains is an important aspect.

Regulation of carbon flux and NADH/NAD supply to enhance 2,3-butanediol production in Enterobacter aerogenes.

As a valuable platform chemical, 2,3-Butanediol (2,3-BDO) has a variety of industrial applications, and its microbial production is particularly attractive as an alternative to petroleum-based production. In this study, the regulation of intracellular carbon flux and NADH/NAD was used to increase the 2,3-BDO production of Enterobacter aerogenes. The genes encoding lactate dehydrogenase (ldh) and pyruvate formate lyase (pfl) were disrupted using the λ-Red recombination method and CRISPR-Cas9 to reduce the production of several byproducts and the consumption of NADH.

Metabolomics reveals the effect of hypobaric treatment on energy metabolism in vibration-injured 'Huangguan' pears.

Mechanical damage caused by vibration during transportation can destroy organization structure and reduce the fruit quality. The objective was to reveal the mechanism of hypobaric treatment on energy metabolism in vibration-injured 'Huangguan' pears based on metabolomics. Results showed that hypobaric treatment delayed the decline of adenosine triphosphate (ATP) content, energy charge (EC), H-ATPase and Ca-ATPase activities comparing to untreated samples.

Infection Process and Genome Assembly Provide Insights into the Pathogenic Mechanism of Destructive Mycoparasite with Host Specificity.

is the pathogen in the white mildew disease of , one of the popular mushrooms. This disease frequently occurs and there is no effective method for disease prevention and control. In the present study, is found to be the only host of , indicating strict host specificity.

Metabolic Engineering of for Improved 2,3-Butanediol Production by Manipulating NADH Levels and Overexpressing the Small RNA RyhB.

Biotechnological production of 2,3-butanediol (2,3-BD), a versatile platform bio-chemical and a potential biofuel, is limited due to by-product toxicity. In this study, we aimed to redirect the metabolic flux toward 2,3-BD in () by increasing the intracellular NADH pool. Increasing the NADH/NAD ratio by knocking out the NADH dehydrogenase genes (/) enhanced 2,3-BD production by up to 67% compared with wild-type .

Comparison of metabolism substances in Cordyceps sinensis and Cordyceps militaris cultivated with tussah pupa based on LC-MS.

The objective of our study was to compare the chemical composition of Cordyceps sinensis (C. sinensis) and Cordyceps militaris (C. militaris) cultivated with tussah pupa by using metabonomics technology in order to clarify the similarity and difference of the two medicinal materials from the whole metabolite level.

Effect of mechanical vibration on postharvest quality and volatile compounds of blueberry fruit.

The objective of the study was to investigate the effect of mechanical vibration on postharvest quality and volatile compounds in blueberries (Vaccinium spp. Berkeley). Ethylene production, respiratory rate, firmness, decay incidence, soluble solids content (SSC), titratable acid content (TAC), flavonoid content, total phenols content (TPC), enzyme activity and volatile compounds of blueberry fruit were determined.

Influence of drop shock on physiological responses and genes expression of apple fruit.

The aim of our study was to investigate the effect of drop shock on physiological responses and genes expression in harvested apple fruit stored at 20 ± 2 °C. Ethylene production, respiratory rate, firmness, soluble solid content, relative electrical conductivity, LOX (lipoxygenase) activity, MDA (malondialdehyde) content, variation of volatile compounds, ethylene biosynthetic genes, and ethylene receptor genes of apple fruit were examined. The results indicated that drop shock observably resulted in the increase of ethylene production, respiratory rate, soluble solid content, relative electrical conductivity, LOX activity, MDA content and gene expression levels in apples.

Effect of ultrasonic treatment combined with 1-methylcyclopropene (1-MCP) on storage quality and ethylene receptors gene expression in harvested apple fruit.

The objective of our study was to investigate the effectiveness of ultrasonic treatment combined with 1-methylcyclopropene (1-MCP) on storage quality and gene expression of postharvest apples compared with ultrasonic or 1-MCP treatment alone. Ethylene production, respiratory rate, firmness, soluble solid content (SSC), peroxidase (POD), superoxide dismutase (SOD), catalase (CAT) activity, and ethylene receptors gene expression of apples were tested. The results indicated 1-MCP or ultrasonic treatment-restrained ethylene production and respiratory rate, improved firmness, suppressed the escalation of SSC, enhanced the activities of POD, SOD, and CAT, suppressed the gene expression level of MdETR1 and MdERS1 compared with the untreated samples.

Effectiveness of lysozyme coatings and 1-MCP treatments on storage and preservation of kiwifruit.

The objective of the present study was to investigate the effectiveness of lysozyme coatings and 1-MCP on storage and preservation of kiwifruit stored at 4 ± 1 °C and 90-95% RH for 20 d. Ethylene production, respiratory rate, decay incidence, weight loss, firmness, chlorophyll, soluble solid, titratable acid, ascorbic acid, total bacterial count, ascorbate peroxidase (APX), superoxide dismutase (SOD) and catalase (CAT) activity of treated kiwifruit were examined. The results showed that lysozyme coatings or 1-MCP treatment inhibited ethylene production and respiratory rate, delayed the increase of decay incidence, weight loss, soluble solid and total bacterial count, improved firmness, chlorophyll, titratable acid, ascorbic acid content, APX, SOD and CAT activity during the storage compared with the untreated kiwifruit in different degree.

Evaluation of 1-methylcyclopropene (1-MCP) treatment combined with nano-packaging on quality of pleurotus eryngii.

The aim of the present study was to investigate the effect of 1-methylcyclopropene (1-MCP) combined with nano-packaging on quality of pleurotus eryngii stored at 4 ± 1 °C for 12 days. Texture, respiration rate, soluble protein, soluble sugar, weight loss, soluble solid, malondialdehyde (MDA) content, polyphenol oxidase (PPO), superoxide dismutase (SOD) and catalase (CAT) activity of treated pleurotus eryngii were determined. The results suggested that 1-MCP treatment combined with nano-packaging reduce respiration rate, weight loss and MDA content, delayed the decrease of soluble protein content, and maintained soluble sugar and soluble solid content of treated pleurotus eryngii during the storage at 4 ± 1 °C compared with the untreated samples.

Modulation of Actinidia arguta fruit ripening by three ethylene biosynthesis inhibitors.

The purpose of this study was to investigate the effect of postharvest application of 1-methylecyclopropene (1-MCP), 1-pentylcyclopropene (1-PentCP) and 1-octylcyclopropene (1-OCP), two structural analogues of 1-MCP, on Actinidia arguta (Siebold et Zucc.) Planch. ex.