Publications by authors named "Fangjuan Liu"
Article Synopsis
- Asthenozoospermia is a major cause of male infertility characterized by reduced sperm motility, but the mechanisms behind this condition are still unclear.
- The study analyzed sperm samples from asthenozoospermic men and healthy controls, identifying 152 differentially expressed proteins, with a focus on the enzyme glucose-6-phosphate isomerase (GPI), which was found to be significantly reduced in the affected samples.
- The research indicates that deficits in energy metabolism, particularly related to GPI, contribute to poor sperm motility, and that adding fructose-6-phosphate can enhance motility in vitro, suggesting new avenues for treatment.
Seminal plasma is a mixture of secretions from several male accessory glands. The seminal plasma contains many secreted proteins which are important for sperm function and male fertility. In this study, we employed N-linked glycosylated peptide enrichment, combined with LC-MS/MS analysis, and establish the first large scale N-linked glycoproteome of human seminal plasma.
View Article and Find Full Text PDFN-Linked glycosylation, a type of post-translational modification, plays important roles in cell-cell recognition, adhesion, and interactions. Although N-linked glycosylated proteins in sperm are known to be important for gamete binding, little is known about the composition of these proteins, particularly glycosylation sites, in humans. In the present study, the use of glyco-FASP, coupled with the tandem mass spectrometry (MS/MS) method, led to the identification of 554 N-glycosylation sites and 297 N-glycosylated proteins in human sperm.
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