Real-time reconstruction using electro-optics modulator-based structured illumination microscopy.

Structured illumination microscopy (SIM), a super-resolution technology, has a wide range of applications in life sciences. In this study, we present an electro-optic high-speed phase-shift super-resolution microscopy imaging system including 2D SIM, total internal reflection fluorescence-SIM, and 3D SIM modes. This system uses galvanometers and an electro-optic modulator to flexibly and quickly control the phase and direction of structured illumination patterns.

Total variation and spatial iteration-based 3D structured illumination microscopy.

Three-dimensional structured illumination microscopy (3D-SIM) plays an essential role in biological volumetric imaging with the capabilities of improving lateral and axial resolution. However, the traditional linear 3D algorithm is sensitive to noise and generates artifacts, while the low temporal resolution hinders live-cell imaging. In this paper, we propose a novel 3D-SIM algorithm based on total variation (TV) and fast iterative shrinkage threshold algorithm (FISTA), termed TV-FISTA-SIM.

Genistein alleviates HO-induced senescence of human umbilical vein endothelial cells via regulating the TXNIP/NLRP3 axis.

Context: Genistein (Gen) has shown protective effects against ageing process.

Objective: To explore the role of Gen on the senescence of HO-induced human umbilical vein endothelial cells (HUVECs) and investigate the possible mechanism.

Materials And Methods: HUVECs were treated with different concentrations of HO (50, 100, 200 and 400 μmol/L) for 1 h or Gen administration (20, 40, 80 and 160 μg/mL) for 24 h.

Identification of Cofilin-1 and Destrin as Potential Early-warning Biomarkers for Gamma Radiation in Mouse Liver Tissues.

Gamma radiation causes cell injury and leads to an increased risk of cancer, so it is of practical significance to identify biomarkers for gamma radiation. We used proteomic analysis to identify differentially expressed proteins in liver tissues of C57BL/6J mice treated with gamma radiation from Cs for 360 d. We confirmed obvious pathological changes in mouse liver tissues after irradiation.

Synthesis of 3-Substituted Aryl[4,5]isothiazoles through an All-Heteroatom Wittig-Equivalent Process.

Extending the previous use of tert-butyl sulfoxide as the sulfinyl source, intramolecular sulfinylation of sulfonamides was successfully performed. The resulting sulfinimides were not isolated and instead were believed to go through an all-heteroatom Wittig-equivalent process to eventually afford aryl[4,5]isothiazoles in high yields.

[Chemical Constituents of Ethyl Acetate Fraction from Hypericum ascyron].

Objective: To study the chemical consituents of Hypericum ascyron.

Methods: The constituents were isolated and purified by chromatography on silica gel; the structure of the compound was determined by MS and NMR spectral analysis.

Results: On the basis of spectroscopic analysis and comparison with the reported data, they were identified as hyperoside( 1),hypercalin B( 2),hypercalin C( 3),1,7-dihydroxyxanthone( 4),2,3-dimethoxyxanthone( 5),1-hydroxy-7-methoxyxanthone( 6),rutin( 7),kaempferol( 8),toxyloxanthone B( 9),quercetin( 10),quercitrin( 11),β-daucosterol( 12) and β-sitosterol( 13).

[Chemical constituents of ethyl acetate fraction from Aeschynanthus bracteatus].

Objective: To study the constituents of EtOAc extract from Aeschynanthus bracteatus.

Methods: The constituents were isolated and purified by chromatography on silica gel and the structures of the compounds were determined by MS and NMR spectral analysis.

Results: On the basis of spectroscopic analysis and comparison with the reported data, compounds were identified as koaburaside (1), coniferaldehyde (2), syringaldehyde (3), sinapinaldehyde (4), 2,4'-dihydroxyacetophenone (5), piceol (6), beta-daucosterol (7), beta-sitosterol (8).