Ethnicity-based analysis of supragingival plaque composition and dental health behaviours in healthy subjects without caries.

Objective: The primary objective of this study was to scrutinise the disparities in the diversity, structure, and function of the oral microbiome among caries-free children from the Zhuang and Han ethnic groups with a focus on the influence of ethnically distinct oral health behaviours on the composition of the oral microbiota.

Methods: A questionnaire survey was conducted to assess oral health behaviours and dental plaque samples were collected from 96 Zhuang and Han children aged 4-5 years living in Guangxi, southern China for high-throughput sequencing. PCR amplification was performed for sequencing of the V4 region of the 16S rDNA gene, and second-generation sequencing was performed using the Illumina MiSeq platform to compare and analyse the diversity, structure and function of the microbiota.

Design and Experimentation of a Machine Vision-Based Cucumber Quality Grader.

The North China type cucumber, characterized by its dense spines and top flowers, is susceptible to damage during the grading process, affecting its market value. Moreover, traditional manual grading methods are time-consuming and labor-intensive. To address these issues, this paper proposes a cucumber quality grader based on machine vision and deep learning.

Comprehensive analysis of the MIR4435-2HG/miR-1-3p/MMP9/miR-29-3p/DUXAP8 ceRNA network axis in hepatocellular carcinoma.

A growing number of studies have shown that competitive endogenous RNA (ceRNA) regulatory networks might play important roles during the process of hepatocellular carcinoma (HCC). This study assessed the role of the ceRNA network in immune cell infiltration in HCC. Immune-related gene sets were downloaded from Molecular Signatures Database, and differentially expressed genes were screened based on TCGA HCC transcriptome data.

Correction: Genetic variations in sterol regulatory element binding protein cleavage-activating protein (SCAP) are associated with blood pressure in overweight/obese Chinese children.

[This corrects the article DOI: 10.1371/journal.pone.

Genetic variations in sterol regulatory element binding protein cleavage-activating protein (SCAP) are associated with blood pressure in overweight/obese Chinese children.

Objective: Previous studies demonstrated a role of variations in sterol regulatory element binding protein (SREBP) cleavage-activating protein (SCAP) in obesity and blood lipids. But the associations between SCAP polymorphisms and blood pressure (BP) are not clear. This study aimed to investigate the relationship between genetic variations in SCAP and BP phenotypes in a Chinese pediatric population.

Gender-Specific Effect of -102G>A Polymorphism in Insulin Induced Gene 2 on Obesity in Chinese Children.

Background. Insulin induced gene 2 (INSIG2) encodes a protein that has a biological effect on regulation of adipocyte metabolism and body weight. This study aimed to investigate the association of INSIG2 gene -102G>A polymorphism with obesity related phenotypes in Chinese children and test gender-specific effects.

GWAS-Identified Common Variants With Nonalcoholic Fatty Liver Disease in Chinese Children.

Objectives: Three genome-wide association studies were previously done for nonalcoholic fatty liver disease (NAFLD) among individuals of Western countries and identified several genetic variants associated with NAFLD. The study aimed to identify whether 7 GWAS-identified common variants (GCKR rs780094, PDGFA rs343064, FDFT1 rs2645424, COL13A1 rs1227756, EHBP1L1 rs6591182, NCAN rs2228603, and PNPLA3 rs738409) were associated with NAFLD in Chinese children.

Methods: This case-control study recruited 1027 Chinese children of age 7 to 18 years, including 162 children with NAFLD and 865 children without NAFLD.

[Association between rs780094 polymorphism in GCKR and plasma lipid levels in children and adolescents].

Objective: To investigate the association between rs780094 polymorphism in glucokinase regulatory protein (GCKR) and plasma lipid levels in children and adolescents.

Methods: 1 026 Chinese children aged 7 to 18 years were recruited, with anthropometric measurements, detection of plasma lipid levels and genotyping of rs780094 performed. Relationships between polymorphism and plasma lipid levels were tested, using multivariate linear regression and logistic regression.

The gene-gene interaction of INSIG-SCAP-SREBP pathway on the risk of obesity in Chinese children.

Background: Childhood obesity has become a global public health problem in recent years. This study aimed to explore the association of genetic variants in INSIG-SCAP-SREBP pathway with obesity in Chinese children.

Methods: A case-control study was conducted, including 705 obese cases and 1,325 nonobese controls.

Association study of childhood obesity with eight genetic variants recently identified by genome-wide association studies.

Background: Being overweight or obese is becoming increasingly common in low- and middle-income countries. The present study aimed to examine association of eight genetic variants with obesity and to estimate the cumulative effects of these variants in Chinese children.

Methods: We conducted the case-control study in a total of 2,030 subjects.

[Association of rs2228314 polymorphism in SREBP2 with serum lipid levels and obesity among children and adolescents].

Objective: To study the relationship between rs2228314 polymorphism in sterol regulatory element binding protein 2 gene (SREBP2) and obesity, serum lipid levels in children and adolescents.

Methods: In our study, 2 030 children and adolescents aged from 7 to 18 years participated. Anthropometric measurements, including height and weight, were performed.

Association of INSIG2 rs9308762 with ALT level independent of BMI.

Objectives: An increasing number of people are at risk for developing nonalcoholic fatty liver disease (NAFLD). Because obesity is a risk factor for NAFLD, the common variants of obesity-susceptible genes may be associated with NAFLD. Our aim was to identify whether the obesity-susceptible gene variants (rs9939609, rs9930506, and rs4783819 in fat mass and obesity-associated gene (FTO); rs12970134 and rs17782313 in melanocortin-4 receptor gene (MC4R); and rs7566605, rs13428113, and rs9308762 in insulin-induced gene 2 [INSIG2]) were associated with NAFLD.

[Association between early onset of menarche and anthropometry measurements among adolescent girls in China].

Objective: To explore the association between early menarche with anthropometry measurements among adolescent girls in China.

Methods: Research material was selected from the data of 2010 Chinese National Surveys on Students Constitution and Health. Probability unit regression method was used to calculate the age of 10th percentile (P 10) at menarche and menarche age before the P 10 was defined as early menarche(9.

[Effects of overweight and obesity on physical function among children and adolescents: a multilevel analysis].

Objective: To analyze the influence of overweight and obesity on body function among children and adolescents using a multilevel model, and to provide the basis for understanding their influence.

Methods: According to the "Reference Norms for Screening Overweight and Obesity in Chinese Children and Adolescents" set up by Working Group Obesity in China (WGOC) in 2003, we screened out individuals with overweight and obesity from the data obtained from the body and health survey of Chinese children and adolescents in 2005. The samples of the non-overweight group and the overweight group were randomly selected from the screened data by 1:1 match with samples of the obesity group by gender and city/rural area, then underwent the multilevel model to explore the influence of overweight and obesity on body function.