Exposure to 6PPD-Q induces dysfunctions of ovarian granulosa cells: Its potential role in PCOS.

N-(1,3-dimethylbutyl)-N'-phenyl-p-phenylenediamine-quinone (6PPD-Q), an environmental pollutant derived from the ozonolysis of the widely used tire rubber antioxidant 6PPD, has been found to accumulate in air, dust, and water, posing significant health risks. While its reproductive toxicity in male organisms has been established, its effects on female reproductive health remain unclear. Polycystic ovary syndrome (PCOS), a common endocrine disorder in premenopausal women, is known to be influenced by environmental pollutants.

Metacognitive processes, situational factors, and clinical decision-making in nursing education: a quantitative longitudinal study.

Objective: This study examined the longitudinal development of metacognitive skills and clinical decision-making abilities in nursing students, focusing on the interactions between metacognitive processes, situational factors, and individual differences.

Methods: A longitudinal, quantitative design was employed, following 185 third-year nursing students from a major university in China over one academic year. Data were collected at six time points using the Metacognitive Awareness Inventory, Nursing Decision-Making Instrument, and custom-designed clinical scenario assessments.

Effects of Blood Flow Restriction Combined with Aerobic Stepping Exercise in Sarcopenia: A Study Protocol for a Randomized Clinical Trial.

Background: Blood flow restriction training (BFRT) can produce effects similar to high-intensity exercise at lower intensities, making it a potentially more suitable method for older adults with sarcopenia. This study aims to determine the efficacy of the intervention on improving physical fitness in older adults with sarcopenia when blood flow restriction (BFR) and aerobic exercise (AE) are combined (BFR-AE) and to explore the related metabolic and signaling mechanisms.

Methods: This is a three-arm, parallel, randomized controlled trial.

The impact of meteorological conditions on cardiovascular and cerebrovascular diseases in different microclimatic zones of low-latitude mountainous areas.

Introduction: Epidemiological evidence suggests cerebrovascular disease (CVD) incidence is correlated to meteorological conditions. However, research on the primary meteorological factors influencing the incidence of CVD and their influence thresholds in low-latitude mountainous regions remains insufficient. We aimed to investigate the association between meteorological conditions and CVD occurrence in Guizhou province.

Baricitinib-loaded EVs promote alopecia areata mouse hair regrowth by reducing JAK-STAT-mediated inflammation and promoting hair follicle regeneration.

Alopecia areata (AA) is a common and recurrent type of hair loss. Despite oral administration of baricitinib exerts a good effect on refractory AA, the long-term administration of baricitinib carries significant side effects, poor compliance, and the efficacy is difficult to maintain after drug withdrawal. Therefore, the exploration of a safe and effective local administration of baricitinib to treat AA is of great clinical importance.

Protective effect of phage pSal-4 on chicken intestinal epithelial cells injured by Salmonella enteritidis.

Salmonella enteritidis plays a significant role in zoonotic infections. This pathogen settles in the intestinal tract of poultry and contaminates meat and egg products during production for slaughter. Consequently, it can also be transmitted to humans along the food chain, posing a significant risk to public health.

Two-dimensional QDs-Co-CuS/TiC/TiO heterojunction with synergistic unsaturated bimetal sites and sulfur vacancies for highly selective photocatalytic CO reduction.

The primary factors that determine the efficiency and selectivity of multi-electron photoreduction of CO include the chemical properties of the active sites, as well as the kinetics of charge separation and transfer. Herein, a novel two-dimensional QDs-Co-CuS/TiC/TiO heterojunction is developed, with Co-CuS quantum dots serving as cocatalysts and TiC MXene as an effective electron transfer channel. The anchoring effect of TiC facilitates the formation of robust TiS bonds with Co-CuS, thereby promoting efficient separation and transfer of photoelectrons to the Co-Cu bimetallic active sites.

Ferroptosis-related prognostic model of mantle cell lymphoma.

Background: Mantle cell lymphoma (MCL) is a B-cell non-Hodgkin's lymphoma. Ferroptosis, an iron-dependent programmed cell death, is closely related to cancer prognosis. In this study, we established a model of ferroptosis related genes for prognostic evaluation of patients with MCL.

Enhancing peroxidase activity of NiCoO nanoenzyme by Mn doping for catalysis of CRISPR/Cas13a-mediated non-coding RNA detection.

CRISPR/Cas13a with precise and controllable programming of endonuclease activity has been served as powerful tool for RNA sensing. Although with high sensitivity, existing CRISPR/Cas13a-based biosensors need complex amplification procedure or special equipment that limited quantification capability. Here, Mn-doped NiCoO (Mn/NiCoO) nanozyme with enhanced peroxidase activity was synthesized and combined with CRISPR/Cas13a-based reaction to develop a simple, sensitive and universal biosensor for RNA detection, which is achieved through target recognition that activates Cas enzymes to cleave RNA reporter for inhibiting Mn/NiCoO nanozyme to assemble on microplate.

The impact of dietary supplementation of Quercetagetin on growth, antioxidant capacity, and gut microbiota of diquat-challenged broilers.

This experiment aimed to investigate the effects of Quercetagetin (QG) on the growth performance, antioxidant capacity, and cecal microbiota of broilers. Two hundred and forty 21-day-old WOD168 broilers with similar body weights were randomly divided into five groups of six replicates each with eight chickens. The control group was fed a basal diet composed of corn and soybean meal, while the experimental groups received basal diets supplemented with 0, 10, 20, and 40 mg/kg QG, along with intraperitoneal injection of 20 mg/kg body weight Diquat (DQ).

Efficacy of oral treprostinil for treating pulmonary arterial hypertension: a systematic review and meta-analysis.

Introduction: Pulmonary arterial hypertension (PAH) is a progressive condition characterized by elevated blood pressure in the pulmonary arteries, leading to significant morbidity and mortality. Treprostinil, a synthetic analogue of prostacyclin, has emerged as a potential treatment option.

Aim: The efficacy and safety of oral treprostinil in PAH patients remain subjects of ongoing research.

Double-Shell Encapsulation of Lead-Free Tin Halide Perovskite for Self-Powered Smart Windows.

Luminescent solar concentrators (LSC) have the potential application in building integrated photovoltaic (BIPV). 0D tin-based perovskites are a promising embedding phosphor in LSC due to the large Stokes shift and high photoluminescence quantum yield. But the instability and uncontrollable crystal growth are severe limiting their successful utilization in device fabrication.

Label-free and ultrasensitive electrochemical detection of nucleic acids based on an exonuclease III-assisted target recycling amplification strategy using a heated gold disk electrode.

The present work demonstrates a label-free, rapid and ultrasensitive electrochemical sensor for specific DNA detection with an exonuclease III (Exo III)-assisted target recycling amplification strategy and elevated electrode temperature at a heated gold disk electrode (HAuDE). The proposed electrochemical DNA (E-DNA) sensor was designed such that in the presence of the target DNA, the electrode self-assembled capture probe hybridizes with the target DNA to form a duplex structure, which triggers Exo III to specifically recognize this structure and selectively digest the capture probe, while the released target DNA underwent recycling to hybridize with a new capture probe, leading to the gradual digestion of a large amount of capture probes. It was found that during the digestion period, the activity of Exo III could be significantly improved by elevating electrode temperature, thus promoting the digestion reaction and improving the sensitivity for target DNA detection.

Investigation of cold-resistance mechanisms in cryophylactic yeast based on comparative transcriptome analysis.

Introduction: Low temperature inhibits the growth of most microorganisms. However, some microbes can grow well in a low temperature, even a freezing temperature.

Methods: In this study, the mechanisms conferring cold resistance in the cryophylactic yeast () MS612, an isolate of the epidermis of ice grapes, were investigated based on comparative transcriptome analysis.

Robust and predictive 3D-QSAR models for predicting the activities of novel oxadiazole derivatives as multifunctional anti-Alzheimer agents.

In recent years, Alzheimer disease (AD) as a neurodegenerative disorder has been increasing annually with the aging of the global population, therefore, development of novel anti-AD drugs is imperative. Studies have proven that glycogen synthase kinase-3β (GSK-3β) is a pivotal factor in the development of AD. Therefore, GSK-3β inhibitors would provide powerful means to treat the disorders, such as AD.

Impact of short-term proton pump inhibitors vs. histamine-2 receptor antagonists on gut microbiota in patients with acute coronary syndrome: A multicenter randomized trial.

Background: Several randomized controlled studies have suggested that the prophylactic use of proton pump inhibitors (PPIs) in intensive care unit (ICU) patients could not reduce the incidence of gastrointestinal bleeding (GIB) and may increase adverse events such as intestinal infection and pneumonia. Gut microbiota may play a critical role in the process. PPIs has been widely prescribed for GIB prophylaxis in patients with acute coronary syndrome (ACS).

The Role of Frustrated Lewis Pair in Catalytic Transfer Hydrogenation of Furfural using Nickel Single-Atom Catalysts: A Theoretical Study.

The burgeoning field of frustrated Lewis pair (FLP) heterogeneous catalysts has garnered significant interest in recent years, primarily due to their inherent ability to activate H-source molecules, thereby facilitating hydrogenation reactions. In this study, non-precious transition metal atoms were anchored onto several models of pyridinic nitrogen incorporated graphene sheet. Theoretical calculations substantiated energy barriers as low as 0.

A novel multi-channel applicator with a U-shaped channel for vaginal intracavity brachytherapy.

Background: Endometrial cancer is one of the most common gynecological malignancies in the world. Vaginal brachytherapy is an important postoperative adjuvant treatment for endometrial cancer. However, a common problem with existing applicators is insufficient dose at the vaginal apex.

Ultrathin 2D-2D MXene-LDH Interlayer with High Polysulfide Adsorption Ability for Advanced Li-S Batteries.

Lithium-sulfur (Li-S) batteries are considered as promising energy storage systems due to the high energy density of 2600 W h kg. However, the practical application of Li-S batteries is hindered by the inadequate conductivity of sulfur and LiS, as well as the shuttle effect caused by polysulfides during the charge-discharge process. Introducing a conductive interlayer between the cathode and the separator to physically resist polysulfides represents an effective and straightforward approach to mitigate the shuttle effect in Li-S batteries.

Construction of ion/electron transfer multi-channels for the composite film electrode from GO and cellulose derived porous carbon in supercapacitor.

Due to excellent flexibility and dispersibility, 2D graphene oxide (GO) is regarded as one of the prospective materials for preparing self-supporting electrode material. Nevertheless, the self-stacking characteristic of GO significantly restricts the ion transmission and accessibility in GO-based electrodes, especially in the direction perpendicular to the electrode surface. Herein, a novel composite film was fabricated from GO and 3D porous carbon (PC) through vacuum filtration combined with thermal reduction strategy.

Effective Detection of Hoechst Side Population Cells by Flow Cytometry.

The side population (SP) cells are identified through Hoechst 33342 staining and analyzed using flow cytometry (FCM). The Hoechst SP method is utilized for the isolation of stem cells based on the dye efflux properties of ATP-binding cassette (ABC) transporters. The method was initially employed for the identification and isolation of hematopoietic stem cells (HSCs), but it has now evolved to primarily focus on the identification and isolation of cancer stem cells (CSCs).