Effects of ttk on development and courtship of male Nilaparvata lugens.

Background: The transcription product of tramtrack (ttk) is an important transcription factor which plays many roles in the regulation of the development, differentiation and chromosome recombination of organisms. Few studies have been reported on the specific functions of ttk in other insects except Drosophila melanogaster. Our aims are to reveal the ttk effects on development and courtship of male rice pest brown planthopper (BPH), Nilaparvata lugens.

Analysis of maternal effect genes from maternal mRNA in eggs of .

To understand how many kinds of mRNAs in female adults can be transferred into the eggs and the molecular basis of embryonic axis specification in we performed de novo transcriptome sequencing of six cDNA libraries of female adults and unfertilized eggs. Total 60,306 unigenes were obtained, with an average length of 1114.51 bp and N50 length of 2112 bp.

Differential DNA methylation between long-winged and short-winged adults of .

a catastrophic rice pest in South East Asia, has adults with wing dimorphism. DNA methylation has been proved to play an important role in regulation of phenotype differentiation in insects. In this study, methylation sensitive amplification polymorphism (MSAP) was used to investigate the cytosine methylation state at CCGG sites in macropterous male adults (MMA) and brachypterous male adults (BMA) of brown planthopper.

Transcriptome profiling and dimorphic expression of sex-related genes in fifth-instar nymphs of Sogatella furcifera, an important rice pest.

Sogatella furcifera is an important rice pest. In order to understand the molecular basis of the sex determination in this pest, we performed de novo transcriptome sequencing of six cDNA libraries (three biological replicates) of female and male fifth-instar nymphs. Total 65,199 unigenes were obtained, with an average length of 971.

De Novo Assembly and Analysis of the White-Backed Planthopper (Sogatella furcifera) Transcriptome.

The white-backed planthopper Sogatella furcifera (Horváth) has become an important pest on rice in China and Southeast Asian countries. White-backed planthopper wing bud length is in relation to adult wing length, but little is known about the development and differentiation of wing buds at the molecular level. Using Illumina HiSeq high-throughput sequencing technology, we sequenced four cDNA libraries, two biological replicates of long-winged female fifth-instar nymphs (LW), and two of short-winged nymphs (SW).

Construction of a synthetic dragline silk gene multimer and its expression in .

One of the most representative core gene sequence of dragline silk protein partial cDNA monomer (JN857964.2) was selected and multimerized using a "head-to-tail" strategy by compatible but nonregenerable sites at both ends resulting in a concatemer of 16 contiguous monomers. This concatemer was cloned into pET-28a(+) expression vector and transformed into .

Analysis of Differential Proteins in Two Wing-Type Females of Sogatella furcifera (Hemiptera: Delphacidae).

Sogatella furcifera(Horvath) is an important rice pest with the wing dimorphism, including macropterous and brachypterous morphs. The protein expression profiles in two wing-type adults and two wing-type disc fifth-instar nymphs were analyzed using two-dimensional gel protein electrophoresis and mass spectrometry. In adults and fifth-instar nymphs, 127 and 162 protein spots were detected, respectively.

Differentially methylated genomic fragments related with sexual dimorphism of rice pests, Sogatella furcifera.

Sogatella furcifera (Hovarth) is a major rice pest with sexual dimorphism. The objective of the current research was to monitor differentially cytosine methylation at CCGG sequences in male and female adults of S. furcifera to determine the association between gene methylation and sexual phenotypes using methylation-sensitive representational difference analysis.

Differential DNA methylation between two wing phenotypes adults of Sogatella furcifera.

Sogatella furcifera is a major rice pest with wing dimorphism. DNA methylation is an important epigenetic modification that plays a role in gene regulation and phenotype variation in most organisms. The objective of the current research was to survey the frequencies and variation of cytosine methylation at CCGG sequences in macropterous female adults (MFA) and brachypterous female adults (BFA) of S.

Two new anti-plasmodial flavonoid glycosides from Duranta repens.

The CHCl(3)-soluble fraction of the whole plant of Duranta repens showed anti-plasmodial activity against the chloroquine-sensitive (D6) and chloroquine-resistant (W2) strains of Plasmodium falciparum, with IC(50) values of 8.5 ± 0.9 and 10.

Antioxidant phenylpropanoid glycosides from Buddleja davidii.

Phytochemical investigations on the n-BuOH-soluble fraction of the whole plant of Buddleja davidii led to the isolation of the phenylpropanoid glycosides 1-10. Their structures were determined by 1D and 2D NMR spectroscopic techniques. All the compounds showed potent antioxidative activity in three different tests, with IC(50) values in the range 4.

Repenins A-D, four new antioxidative coumarinolignoids from Duranta repens Linn.

Phytochemical investigations on the CHCl(3)-soluble fraction of the whole plant of Duranta repens Linn. led to the isolation of four new coumarinolignoids, Repenins A-D (1-4), along with the known coumarinolignoids, cleomiscosin A (5) and durantin A (6). Their structures were determined by modern spectroscopic analysis including 1D and 2D NMR techniques and chemical studies.

Purification and characterization of native and recombinant SaPIN2a, a plant sieve element-localized proteinase inhibitor.

SaPIN2a encodes a proteinase inhibitor in nightshade (Solanum americanum), which is specifically localized to the enucleate sieve elements. It has been proposed to play an important role in phloem development by regulating proteolysis in sieve elements. In this study, we purified and characterized native SaPIN2a from nightshade stems and recombinant SaPIN2a expressed in Escherichia coli.

Effects of crowding, isolation, and transfer from isolation to crowding on total ecdysteroid content of eggs in Schistocerca gregaria.

We examined the ecdysteroid content of eggs from crowd-reared and solitary-reared desert locusts, Schistocerca gregaria, throughout embryogenesis from the day of egg laying until shortly before hatching on day 14. Depending on the time during incubation, ecdysteroid content in eggs from crowd-reared females was 5-10 times higher than in eggs from solitary-reared females. Our investigation revealed two peaks in ecdysteroid content of eggs from crowd-reared females, a small one at day 3 and a major peak at day 10 of incubation.