[Preparation and preliminary application of the polyclonal antibody against dense granule protein 24].

Objective: To prepare and characterize the mouse polyclonal antibody against the dense granule protein 24 (GRA24) of , and explore its preliminary applications.

Methods: The GRA24 coding sequences of different strains were aligned using the MEGA-X software, and the dominant peptide of the GRA24 protein was analyzed with the Protean software. The base sequence encoding this peptide was amplified using PCR assay and ligated into the pET-28a vector, and the generated GRA24 truncated protein was transformed into BL21.

Enhanced Hygrothermal Stability of In-Situ-Grown MAPbBr Nanocrystals in Polymer with Suppressed Desorption of Ligands.

Currently, the intrinsic instability of organic-inorganic hybrid perovskite nanocrystals (PNCs) at high temperature and high humidity still stands as a big barrier to hinder their potential applications in optoelectronic devices. Herein, we report the controllable in-situ-grown PNCs in polyvinylidene fluoride (PVDF) polymer with profoundly enhanced hygrothermal stability. It is found that the introduced tetradecylphosphonic acid (TDPA) ligand enables significantly improved binding to the surface of PNCs via a strong covalently coordinated P-O-Pb bond, as evidenced by density functional theory calculations and X-ray photoelectron spectroscopy analyses.

[Bioinformatics analysis and prokaryotic expression of serine protease inhibitor 1].

Objective: To predict the structure and antigenic epitope of the serine protease inhibitor 1 (Ss-SRPN-1) protein using bioinformatics tools, and to construct prokaryotic expression plasmids for expression of recombinant Ss-SRPN-1 protein, so as to provide the basis for unraveling the function of the Ss-SRPN-1 protein.

Methods: The amino acid sequence of the Ss-SRPN-1 protein was downloaded from the NCBI database, and the physicochemical properties, structure and antigenic epitopes of the Ss-SRPN-1 protein were predicted using bioinformatics tools, including ExPASy, SWISS-MODEL and Protean. Primers were designed according to the nucleotide sequences of Ss-SRPN-1, and the gene was amplified, cloned and sequenced with genomic DNA extracted from the infective third-stage larvae of as a template.

[Progress of researches on co-infection with other pathogens].

is an opportunistic pathogenic parasite that can cause severe strongyloidiasis and even death among immunocompromised individuals. Previous clinical studies have reported cases co-infected with and other pathogens, such as parasites, viruses, bacteria and fungi. This review summarizes strongyloidiasis patients co-infected with pathogens, and analyzes the impact of co-infection on strongyloidiasis, so as to provide insights into the reduction of the morbidity and mortality of disorders associated with infections.

[Expression of CD47 and its ligands in pregnant mice infected with during pregnancy].

Objective: To investigate the dynamic expression of cluster of differentiation 47 (CD47) and its ligands signaling regulatory protein α (SIRPα) and thrombospondin-1 (TSP-1) in mice infected with in the second and third trimesters.

Methods: C57BL/6J mice (6 to 8 weeks old) were used for modeling infection in the first trimester, and the pregnant mice were randomly divided into the normal control and infection groups, of 10 mice in each group. Pregnant mice in the infection group were intraperitoneally injected with 150 tachyzoites on gestational day (Gd) 6.

[Expression of hypoxia-inducible factor-1 in mice infected with during pregnancy].

Objective: To investigate the expression and possible role of hypoxia-inducible factor-1 (HIF-1) at the maternal-fetal interface following infection during early pregnancy.

Methods: Twenty pregnant C57BL/6 mice, each weighing 16 to 20 g, were randomly divided into 4 groups, including the 12-d control group, 12-d infection group, 18-d control group and 18-d infection group. Mice in the 12-d and 18-d infection groups were injected intraperitoneally with 150 tachyzoites of the PRU strain on day 6 of pregnancy, while mice in the 12-d control and 18-d control groups were injected with the same volume of phosphate buffered saline (PBS).

High-Performance Potassium-Ion Batteries with Robust Stability Based on N/S-Codoped Hollow Carbon Nanocubes.

Currently, a big challenge for the practical use of potassium-ion batteries (PIBs) is their intrinsically poor cycling stability, due to the relatively large radius of K and sluggish kinetics for intercalation/deintercalation. Here we report the scalable fabrication of N/S-codoped hollow carbon nanocubes (NSHCCs), which have the potential as an electrode for advanced PIBs with robust stability. Their discharge and charge specific capacities are ∼560 mA h g and 310 mA h g at a current density of 50 mA g, respectively.

Robust high-temperature potassium-ion batteries enabled by carboxyl functional group energy storage.

The popularly reported energy storage mechanisms of potassium-ion batteries (PIBs) are based on alloy-, de-intercalation-, and conversion-type processes, which inevitably lead to structural damage of the electrodes caused by intercalation/de-intercalation of K with a relatively large radius, which is accompanied by poor cycle stabilities. Here, we report the exploration of robust high-temperature PIBs enabled by a carboxyl functional group energy storage mechanism, which is based on an example of p-phthalic acid (PTA) with two carboxyl functional groups as the redox centers. In such a case, the intercalation/de-intercalation of K can be performed via surface reactions with relieved volume change, thus favoring excellent cycle stability for PIBs against high temperatures.

[PD-1/PD-L1 expression and its interaction with interferon-γ in -infected mice at middle and late pregnancy].

Objective: To explore the dynamic expression of programmed cell death-1 (PD-1) and its ligand PD-L1 at the maternal-fetal interface of mice post-infection with at early pregnancy and examine its interaction with interferon-γ (IFN-γ).

Methods: A total of 20 mice at day 0 of pregnancy were randomly assigned into 4 groups, including the 12-day pregnancy control group (12 dpn group), 12-day pregnancy and infection group (12 dpi group), 18-day pregnancy control group (18 dpn group) and 18-day pregnancy and infection group (18 dpi group), respectively. On the 6th day of the pregnancy, mice in the 12 dpi and 18 dpi groups were injected intraperitoneally with 150 tachyzoites of the PRU strain, while mice in the 12 dpn and 18 dpn groups were injected with the same volume of PBS.

[Expression and clinicopathological significance of and genes in colorectal cancer patients complicated with schistosomiasis].

Objective: To investigate the expression and clinicopathological significance of and genes in colorectal cancer (CRC) patients complicated with schistosomiasis.

Methods: The CRC patients receiving surgical treatment in the First Affiliated Hospital of Dali University from June 2016 to June 2020 were recruited as the study subjects, and 30 subjects were randomly sampled from the CRC patients complicated with schistosomiasis (CRC-S group) and 30 subjects were randomly sampled from the CRC patients without schistosomiasis (CRC group) using a random number table method. The cancer specimens were sampled from subjects in the CRC-S and CRC groups, and the peri-cancer specimens were sampled from subjects in the CRC group.

[Study on the mechanisms of the intestinal tight-junction destruction caused by infection in rats].

Objective: To explore the mechanism of the intestinal barrier damage caused by infections in rats.

Methods: Thirty SD rats were randomly divided into the control group, and the 1-, 3-, 6- and 9-week-infection groups, of 6 rats in each group. Rats in each infection group were orally infected with trophozoites at a density of 2 × 10 parasites per rat, and the control group was given an equal volume of phosphate buffered saline solution.

[Evaluation of the effect of the integrated echinococcosis control program in Ningxia Hui Autonomous Region from 2011 to 2018].

Objective: To evaluate the effect of the integrated echinococcosis control program in Ningxia Hui Autonomous Region from 2011 to 2018.

Methods: A package of integrated interventions were employed for echinococcosis control in 22 counties (districts) of Ningxia Hui Autonomous Region from 2011 to 2018, including screening of human echinococcosis, treatment of echinococcosis patients, deworming of domestic dogs and monitoring of infections, surveillance of echinococcosis in bovines and sheep, health education. The detection of human echinococcosis, seroprevalence of anti- antibody in children at ages of 6 to 12 years, the coproantigen-positive rate in domestic dogs, prevalence of echinococcosis in bovines and sheep, and the awareness of echinococcosis control knowledge were investigated and compared during the period between 2011 and 2018.

[Treatment of infection with traditional Chinese medicine: a case report].

This paper reports a case with infections, and summarizes the diagnosis and treatment with traditional Chinese medicine.

[Surveillance of soil-borne nematodiasis in Danyang City from 2012 to 2018].

Objective: To monitor the epidemic status of human soil-borne nematodiasis in Danyang City, so as to provide scientific evidence for formulating control measures.

Methods: The soil-borne nematode infections were tested using a modified Kato-Katz technique among residents aged more than 3 years living in Danyang City from 2012 to 2018, and the eggs were detected using the transparent adhesive tape anal swab method among children at ages of 3 to 12 years.

Results: A total of 13 540 residents were monitored for soil-borne nematode infections from 2012 to 2018, and the prevalence of human soil-borne nematode infections was 0.

[Study of schistosomiasis transmission interruption based on intensive agriculture].

To study the effect of schistosomiasis transmission interruption model based on intensive agriculture in hilly endemic areas, so as to provide the reference for the similar endemic areas. Based on the development of intensive agriculture in Guanghan City, a comprehensive demonstration area of schistosomiasis control with measures such as new rural construction, hardening ditches, the adjustment of industrial structure and water remediation measures was constructed. Jinhua, Shiguan and Hongyan villages were chosen as the evaluation sites to comparatively analyze the indexes of intensive agriculture and schistosomiasis control effects.

Thermosensitive chitosan-based hydrogels releasing stromal cell derived factor-1 alpha recruit MSC for corneal epithelium regeneration.

Unlabelled: Corneal epithelium integrity depends on continuous self-renewing of epithelium and connections between adjacent cells or between the cells and the basement membrane. Self-renewing epithelium cells mainly arise from the continuous proliferation and differentiation of the basal layer and limbal stem cells. The aim of the present study was to generate a bioactive, thermosensitive chitosan-gelatin hydrogel (CHI hydrogel) by incorporating exogenous recombinant human stromal cell-derived factor-1 alpha (SDF-1 alpha) for corneal epithelium regeneration.

Construction of a Corneal Stromal Equivalent with SMILE-Derived Lenticules and Fibrin Glue.

The scarcity of corneal tissue to treat deep corneal defects and corneal perforations remains a challenge. Currently, small incision lenticule extraction (SMILE)-derived lenticules appear to be a promising alternative for the treatment of these conditions. However, the thickness and toughness of a single piece of lenticule are limited.

[Analysis of capability of examining helminthes: National technique competition of parasitic disease diagnosis in 2013].

Objectives: To understand the ability of worm detection of staff of centers for disease control and prevention (CDC) in China, so as to promote the ability construction.

Methods: In each province, four competitors were selected from the institutions of parasitic diseases control to attend the National Technique Competition for Parasitic Disease Diagnosis and the results of competition were collected and analyzed.

Results: The average scores of microscopic identification and slide preparation of all the 124 competitors were 27.

[Effect of schistosomiasis control in towns reached criteria of infection controlled in Nanchang City].

Objective: To evaluate the effect of schistosomiasis control in towns that had reached the criteria of infection controlled in Nanchang City.

Methods: The residents and livestock of 14 towns that had reached the criteria of infection controlled in Nanchang, Xinjian and Jinxian counties of Nanchang City were sampled to investigate the schistosomiasis endemic situation, Oncomelania hupensis snail status, and data of schistosomiasis control work.

Results: The positive rate of serum tests of the residents was 2.

[Expression of Tim-3 on peripheral blood CD4+ T cells and its correlation with liver function damage parameters in advanced schistosomiasis patients].

Objective: To explore the expression levels of T cell immunoglobulin and mucin domain protein 3 (Tim-3) on peripheral blood CD4+ T cells and its correlation with liver function damage parameters in advanced schistosomiasis patients.

Method: Totally 28 advanced schistosomiasis patients were selected as study subjects, and 20 chronic schistosomiasis patients and 30 healthy persons were selected as controls. The expression levels of Tim-3 on CD4+ T cell were detected by flow cytometry, and the serum IFN-γ and IL-4 levels were detected by ELISA.

[Isolation and identification of Toxoplasma gondii strains from cats in Xuzhou region].

Objective: To isolate Toxoplasma gondii (T. gondii) strains from stray cats and explore their prevalence in Xuzhou City.

Methods: The sera of 41 stray cats were collected to detect the antibodies of T.

[Endemic status of schistosomiasis in People's Republic of China in 2013].

This report presented the endemic status of schistosomiasis in the People's Republic of China at national level in 2013. By the end of 2013, there are 184 943 schistosomiasis japonica cases. Meanwhile, nine acute cases were reported, and 4 cases reduced compared with that of 2012.

[Schistosomiasis status of staff in Hydrology Bureau of Yangtze Water Resources Committee in 2013].

Objective: To understand the status of schistosomiasis of staff in the Hydrology Bureau of Yangtze Water Resources Committee and the Oncomelania hupensis snail condition of their work areas in 2013, so as to provide the evidences for the schistosomiasis control in the industry.

Methods: The physical examination data about schistosomiasis of the staff from 2006 to 2013 were collected and analyzed to understand the schistosomiasis prevalence condition of the staff and the changes of their liver parenchyma. Meanwhile, the snail status in the work areas was surveyed.