Evaluation of Chelator-to-Antibody Ratio on Development of Zr-iPET Tracer for Imaging of PD-L1 Expression on Tumor.

Zr-iPET has been widely used for preclinical and clinical immunotherapy studies to predict patient stratification or evaluate therapeutic efficacy. In this study, we prepared and evaluated Zr-DFO-anti-PD-L1-mAb tracers with varying chelator-to-antibody ratios (CARs), including Zr-DFO-anti-PD-L1-mAb_3X (), Zr-DFO-anti-PD-L1-mAb_10X (), and Zr-DFO-anti-PD-L1-mAb_20X (). The DFO-anti-PD-L1-mAb conjugates with varying CARs were prepared using a random conjugation method and then subjected to quality control.

Automated identification of single and clustered chromosomes for metaphase image analysis.

Background: Chromosome analysis is laborious and time-consuming. Automated methods can significantly increase the efficiency of chromosome analysis. For the automated analysis of chromosome images, single and clustered chromosomes must be identified.

Intercomparison of conventional and QuickScan dicentric scoring for the validation of individual biodosimetry analysis in Taiwan.

Purpose: The dicentric chromosome assay (DCA), the gold standard for radiation biodosimetry, evaluates an individual absorbed radiation dose by the analysis of DNA damage in human lymphocytes. The conventional (C-DCA) and QuickScan (QS-DCA) scoring methods are sensitive for estimating radiation dose. The Biodosimetry Laboratory at Institute of Nuclear Energy Research (INER), Taiwan, participated in intercomparison exercises conducted by Health Canada (HC) in 2014, 2015 and 2018 to validate the laboratory's accuracy and performance.