Kuicolong-yu enema decoction retains traditional Chinese medicine enema attenuates inflammatory response ulcerative colitis through TLR4/NF-κB signaling pathway.

Background: Ulcer colitis (UC) is a chronic, nonspecific, and noninfectious inflammatory bowel disease. Recently, Toll-like receptors (TLRs) have been found to be closely associated with clinical inflammatory diseases. Achieving complete remission in patients with intermittent periods of activity followed by dormancy is challenging.

Circ_0068481 Affects the Human Pulmonary Artery Smooth Muscle Cells' Progression by miR-361-3p/KLF5 Axis.

Background: Uncontrolled proliferation of pulmonary artery smooth muscle cells (PASMCs) contributes to the pathogenesis of pulmonary arterial hypertension (PAH). In this work, we defined the precise part of circ_0068481 in PASMC proliferation and migration induced by hypoxia. We hypothesized that circ_0068481 enhanced hypoxia-induced PASMC proliferation, invasion, and migration through the microRNA (miR)-361-3p/Krüppel-like factor 5 (KLF5) pathway.

[Effect of heat-reinforcing needling on the inflammation and necrotizing apoptosis of synovial cells in synovial tissues of knee joint in rabbits with cold syndrome rheumatoid arthritis].

Objective: To observe the effect of heat-reinforcing needling (HRN) on inflammatory factors and necrotizing apoptosis of synovial cells in synovial tissues of knee joint in rabbits with cold syndrome rheumatoid arthritis (RA), so as to explore its underlying mechanisms in treating RA.

Methods: By using the random number table method, 40 New Zealand rabbits were randomly divided into normal, model, antagonist(AG), twist-reinforceing needling (TRN) and HRN groups, with 8 rabbits in each group. The model of cold syndrome RA was established by ovalbumin induction combined with Freund's complete adjuvant injection and cryogenic freezing method.

[Effect of heat-reinforcing needling on expression of serum inflammatory factors and autophagy of knee joint synovial tissue in rheumatoid arthritis rabbits with cold syndrome].

Objective: To observe the effect of heat-reinforcing needling on the expression of serum inflammatory factors and autophagy of knee synovial tissue in rheumatoid arthritis (RA) rabbits with cold syndrome, so as to explore its mechanism of anti-inflammatory in the treatment of RA.

Methods: Fifty rabbits were randomly divided into normal, model, heat-reinforcing needling, inhibitor and agonist groups (=10 rabbits in each group). The model of RA with cold syndrome was established by Freund's adjuvant and ovalbumin mixed solution injection combined with freezing and wind-cold dampness method.

Comparison of Bran-Processed and Crude Effects on Spleen Deficiency Syndrome in Rats.

Background: The rhizome of (AL) is usually used for the treatment of various diseases such as spleen deficiency syndrome (SDS). Both bran-processed and crude AL is included in Chinese Pharmacopoeia. The different efficacies of bran-processed and crude AL on SDS are largely unknown, and the mechanisms of AL effects have not been fully elucidated.

[Influence of Heat-reinforcing Needling on Expression of Plasma mRNA and mRNA in Patients with Rheumatoid Arthritis of Wind-cold-damp Retention Type].

Objective: To observe influences of heat-reinforcing needling (HRN) on scores of traditional Chinese medicine (TCM) symptoms and expression of plasma ATP synthase subunit O () mRNA and lysosomal V 1 subunit B 2 () mRNA in patients with wind-cold-damp retention type rheumatoid arthritis (RA), so as to investigate its biological mechanisms in "heat production".

Methods: Sixty wind-cold-damp retention type RA patients were randomly allocated to HRN group (=30) and control group (=30). Guanyuan (CV 4), Qihai (CV 6), bilateral Zusanli (ST 36), and local acupoints near the knee-joint were selected for needling stimulation.

[Efficacy observation of xerophthalmia treated with acupuncture of warming-promotion needling technique].

Objective: To observe the differences in the efficacy on xerophthalmia treated with the needling technique for warming-promotion at Fengchi (GB 20) as the main acupoint in comparison with the conventional needling technique.

Methods: Thirty cases were randomly divided into a warming-promotion needling group (n = 15) and a routine needling group (n = 15). In warming-promotion needling group, the needling technique for warming-promotion was applied to Fengchi (GB 20).

[Discussion on the innovative needling methods of "warm reinforcing technique" and "cold reducing technique" invented by professor ZHENG Kui-shan].

The innovated needling methods of "warm reinforcing technique" and "cold reducing technique" by professor ZHENG Kui-shan was analyzed. The discussion on these methods was done in comparison with the techniques of "setting the mountain on fire" and "penetrating the heaven coolness". The innovations and the clinical application advantages were explored.

[Discussion on the important role of Chuaixue in acupuncture manipulation].

Combined with ancient literature, the authors put forward three roles of Chuaixue in acupuncture manipulation. First, it can ascertain the location of the acupoints, divert the patient's attention and alleviate the sufferings of patient. The second one, it can consolidate ying, wei, qi and blood system.

[Observation on the analgesic effect of heat-reinforcing needling manipulation for acute arthritis and the concomitant changes of beta-EP and PGE2 contents in the local joint tissue in rabbits].

Objective: To observe the analgesic effect of heat-reinforcing needling manipulation for acute inflammatory arthritis and its underlying mechanism in experimental rheumatoid arthritis rabbits.

Methods: A total of 60 rabbits were randomized into control (n=6), model (n=6), needle-twirling (n=24) and heat-reinforcing (n=24) groups, and the later 2 groups were further divided into 0 h, 0.5 h, 1 h and 2 h subgroups,with 6 cases in each.

[Analysis on infusing and retaining qi from eight methods of treating diseases in Jinzhen Fu].

Through study of Internal Classic and Yijing (The Book of Changes) combined with clinical experiences, the author analyzes characteristics of indications treated by eight methods in Jinzhen Fu ,manipulations as well as clinical applications of infusing and retaining qi, and believes that indications treated by eight methods includ coldness, heat, deficiency, excess and illness of qi, blood, body liquid(viscera), as well as meridians, collaterals, blood vessels. And eight methods have respective speciality according to their corresponding indications. The manipulations of eight methods, expecially infusing and retaining qi, are complete.

[Brief talk on manipulation and clinical application of Yang Ji-zhou's "through joint crossing meridians" needling method].

Through repeated study on the "through joint crossing meridians needling method" in Zhenjiu Dacheng and clinical practice, it is held that it seems hard to induce qi moving along the meridian through joint to the affected region according to the statement of "through joint crossing meridians needling method" in Zhenjiu Dacheng. If "through joint crossing meridians needling method", "activating qi method" and "qi-lifting method" are combined, the possibility of qi constantly flowing centrality through joint from the remote acupoint at four limbs to the affected region will be distinctly elevated. Clinically, the authors used the "through joint crossing meridian" needling method, attaining qi reaching to the affected region, and acupuncture at only one point can achieve a better therapeutic effect.

[Clinical observation on warming-removing obstruction needling method for treatment of sudden tinnitus and deafness].

Objective: To compare therapeutic effects of acupuncture at Fengchi (GB 20) as main with warming-removing obstruction needling method and routine acupuncture on sudden tinnitus and deafness.

Methods: Sixty-two cases were randomly divided into a warming-removing obstruction needling method group (n=32) and a routine acupuncture group (n=30). The warming-removing obstruction needling method group were treated with acupuncture at Fengchi (GB 20) as main point with warming-removing obstruction needling method, in combination with local, distant points of ear with twirling uniform reinforcing-reducing method used; and the routine acupuncture group were treated with acupuncture at local and distant points of ear with twirling uniform reinforcing-reducing method adopted.

[Summarization of clinical application laws of professor ZHENG Kui-shan's "Wentong needling method"].

"Wentong needling method" is professor ZHENG Kui-shan's needling manipulation of original creation for treatment of various difficult and complicated cases with distinguishing feature in clinical practice of dozens of years, which has both reinforcing and reducing actions, with functions of promoting the flow of qi by warming the channels, removing the phlegm, dispelling wind and cold, promoting flow of qi and blood circulation, and strengthening the body resistance to eliminate pathogenic factors. Clinically, it is used for treatment of difficult diseases such as coronary disease, diseases of the head, face and five sense organs, brain diseases, and wind-cold-dampness arthralgia, etc. with good results.

[Professor Zheng Kui-Shan the father of modern study of Chinese acupuncture and moxibustion and needling methods].

Professor ZHENG Kui-shan is a famous specialist of acupuncture and moxibustion sciences, and he has obtained a lot of achievements in about 70 years' clinical treatment, scientific studies and teaching. He has advocated and devoted himself to the studies of traditional needling methods of acupuncture and moxibustion, with outstanding contributions to the development of Chinese and world's acupuncture and moxibustion. He participated in the scientific studies, clinical treatment and teaching of acupuncture and moxibustion at the early stage of founding of the China Academy of TCM.