[PDGFRα Cell-Derived SCF Regulates Hematopoiesis of Adult Mice].

Objective: To investigate the effect of PDGFRα stromal cells derived SCF on hematopoiesis of adult mice.

Methods: Pdgfrα-CreER; R26-tdTomato mice model was constructed, and the proportion and distribution of PDGFRα cells in the liver, spleen, lung, kidney and bone marrow were analyzed by flow cytometry and confocal microscopy. Then the Pdgfrα-CreER; Scf mice model was further constructed, the Scf in PDGFRα was knocked out specifically, the effect of Scf-knocked out in PDGFRα stromal cells in the propitiation of HSPCs in the bone marrow was analyzed by flow cytometry.