Two-Photon-Excited Fluorescent Tetrabranched Triphenylborane Featuring the Cooperative Effect of Branching in Two-Photon Absorption.

We here disclose a new type of two-photon-excited fluorescent triarylborane, tetrabranched triphenylborane , which contains four electron-donating [4-(,-diphenylamino)phenyl]ethynyl branches at 2,6-positions of two phenyl rings. The cross section of reaches 275 GM (1 GM = 10 cm s photon) in tetrahydrofuran. Compared with dibranched triphenylborane , the 2-fold increase in the number of electron-donating branches induces a 3.

Expression, purification, and improved antigenic specificity of a truncated recombinant bp26 protein of Brucella melitensis M5-90: a potential antigen for differential serodiagnosis of brucellosis in sheep and goats.

Antibodies produced in animals vaccinated using live attenuated vaccines against Brucella spp. are indistinguishable using current conventional serological tests from those produced in infected animals. One potential approach is to develop marker vaccines in which specific genes have been deleted from parental vaccine strains that show good immunogenicity and vaccine efficacy.