Publications by authors named "Fang-Hao Guo"
Aims: MicroRNA-126 (miR-126), one of the most abundant microRNAs in platelets, is involved in the regulation of platelet activity and the circulating miR-126 is reduced during antiplatelet therapy. However, whether intraplatelet miR-126 plays a role in thrombosis and platelet inhibition remains unclear.
Methods And Results: Here, using tissue-specific knockout mice, we reported that the deficiency of miR-126 in platelets and vascular endothelial cells significantly prevented thrombosis and prolonged bleeding time.
Article Synopsis
- Endothelial cells (ECs) are crucial for blood vessel formation and remodeling, but their diversity during development is not well understood; this study aims to explore the transcriptional differences among embryonic ECs and how these change when the microRNA-126 gene is specifically removed.
- Using advanced techniques like single-cell RNA sequencing, researchers identified 11 distinct groups of embryonic ECs, noting their greater growth potential compared to adult ECs; disruptions caused by miR-126 knockout were linked to specific vascular problems and metabolic changes.
- The findings suggest that EC heterogeneity starts in early development and that loss of miR-126 leads to significant disorganization and dysfunction in certain EC populations, which can be partially reversed by introducing
PIWI proteins are key regulators of germline and somatic stem cells throughout different evolutionary lineages. However, how PIWI proteins themselves are regulated remains largely unknown. To identify candidate proteins that interact with PIWI proteins and regulate their stability, here we established a yeast two-hybrid (Y2H) assay in the planarian species We show that DNAJA1, a heat shock protein 40 family member, interacts with the PIWI protein SMEDWI-2, as validated by the Y2H screen and co-immunoprecipitation assays.
View Article and Find Full Text PDFRegeneration, relying mainly on resident adult stem cells, is widespread. However, the mechanism by which stem cells initiate proliferation during this process in vivo is unclear. Using planarian as a model, we screened 46 transcripts showing potential function in the regulation of local stem cell proliferation following 48 h regeneration.
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