Structural and functional study for tRNA cleavage by Glycine o-phenanthroline Cu complex, [CuCl(phen)(gly)]∙4HO.

The o-phenanthroline gly Cu(II) complex, [CuCl(phen)(gly)]∙4HO 1, has been prepared and structurally characterized. The transfer RNA binding and degradation properties of complex 1 have been investigated by UV-vis spectroscopy, cyclic voltammetry (CV), capillary electrophoresis (CE) and atomic force microscopy (AFM) methods. The results showed that 1 can efficiently cleave tRNA in the physiological conditions (pH 7.

Volumetric magnetic resonance imaging analysis in patients with short-term remission of Cushing's disease.

Objective: The data on patients with short-term remission of Cushing's disease (CD) might provide information that is not available from previous long-term remission studies. We aimed to investigate structural changes in the brain in these patients and to examine whether these changes were associated with clinical characteristics.

Design: A cross-sectional study was performed.

Altered gray and white matter microstructure in Cushing's disease: A diffusional kurtosis imaging study.

Exposure to chronic hypercortisolism has multiple adverse effects on brain biology in humans. Cushing's disease (CD) represents a unique and natural human model for examining the effects of hypercortisolism on the brain. This cross-sectional study used Diffusional Kurtosis Imaging (DKI) to investigate the microstructure alterations in both white matter (WM) and gray matter (GM) of CD patients and to determine the relationship of these changes with clinical characteristics.

Altered spontaneous brain activity in Cushing's disease: a resting-state functional MRI study.

Context And Objective: Cushing's disease (CD) provides a unique and naturalist model for studying the influence of hypercortisolism on the human brain and the reversibility of these effects after resolution of the condition. This cross-sectional study used resting-state fMRI (rs-fMRI) to investigate the altered spontaneous brain activity in CD patients and the trends for potential reversibility after the resolution of the hypercortisolism. We also aim to determine the relationship of these changes with clinical characteristics and cortisol levels.

Inhibition of Ubiquitin-specific Peptidase 8 Suppresses Adrenocorticotropic Hormone Production and Tumorous Corticotroph Cell Growth in AtT20 Cells.

Background: Two recent whole-exome sequencing researches identifying somatic mutations in the ubiquitin-specific protease 8 (USP8) gene in pituitary corticotroph adenomas provide exciting advances in this field. These mutations drive increased epidermal growth factor receptor (EGFR) signaling and promote adrenocorticotropic hormone (ACTH) production. This study was to investigate whether the inhibition of USP8 activity could be a strategy for the treatment of Cushing's disease (CD).

[A comparative study on psychological and behavioral development in children aged 1 to 3 years fostered by grandparents and by parents].

Objective: To investigate the differences of psychological and behavioral development between children aged 1 to 3 years fostered by grandparents and those by parents.

Methods: Psychological and behavioral development of 443 children aged 1 to 3 years fostered by their grandparents and of aged-matched 443 children fostered by their parents were assessed with DST, an intellectual developmental screening test developed by Pediatric Hospital of Fudan University in Shanghai.

Results: The abilities of social adaptation and intelligence development in children fostered by their grandparents were obviously retarded as compared with those in children fostered by their parents.

3-(4-Meth-oxy-benzyl-idene)-1,5-dioxa-spiro-[5.5]undecane-2,4-dione.

In the title mol-ecule, C(17)H(18)O(5), which was prepared by the reaction of (R)-1,5-dioxaspiro-[5.5]undecane-2,4-dione and 4-meth-oxy-benzaldehyde with ethanol, the 1,3-dioxane ring is in a distorted envelope conformation with the spiro C atom forming the flap. The crystal structure is stabilized by weak inter-molecular C-H⋯O hydrogen bonds.

(E)-N'-[4-(Methyl-sulfan-yl)benzyl-idene]furan-2-carbohydrazide monohydrate.

In the title compound, C(13)H(12)N(2)O(2)S·H(2)O, the dihedral angle between the aromatic rings is 35.34 (19)° and an intra-molecular N-H⋯O hydrogen bond generates an S(5) ring. In the crystal, mol-ecules are linked by N-H⋯O and O-H⋯O hydrogen bonds, generating (001) sheets.

N'-(3,4-Dimethyl-benzyl-idene)furan-2-carbohydrazide.

The title compound, C(14)H(14)N(2)O(2), was prepared by the reaction of 3,4-dimethyl-benzaldehyde and furan-2-carbohydrazide. The dihedral angle between the aromatic rings is 35.48 (14)°.

N'-Benzyl-idene-furan-2-carbohydrazide.

In the title compound, C(12)H(10)N(2)O(2), the dihedral angle between the benzene ring and the furan ring is 24.6 (2)°. In the crystal, mol-ecules are linked by N-H⋯O hydrogen bonds, generating C(4) chains propagating in [010].

N'-(4-Hy-droxy-benzyl-idene)thio-phene-2-carbohydrazide.

In the title compound, C(12)H(10)N(2)O(2)S, the dihedral angle between the benzene and thio-phene rings is 23.34 (16)°. In the crystal structure, mol-ecules are linked by N-H⋯O and O-H⋯O hydrogen bonds, forming (100) sheets.

4-Ethyl-1-[4-(methyl-sulfan-yl)benzyl-idene]thio-semicarbazide.

There are four independent mol-ecules in the asymmetric unit of the title compound, C(11)H(15)N(3)S(2), with different conformations: the dihedral angles between the benzene rings and thio-urea units are 16.85 (9), 0.56 (10), 8.

4-Ethyl-1-(4-meth-oxy-benzyl-idene)thio-semicarbazide.

In the title compound, C(11)H(15)N(3)OS, the dihedral angle between the aromatic ring and the thio-urea unit is 4.28 (7)° and an intra-molecular N-H⋯N hydrogen bond generates an S(5) ring. In the crystal, mol-ecules are linked into (001) sheets by N-H⋯S hydrogen bonds.

N'-(4-Cyano-benzyl-idene)furan-2-carbohydrazide monohydrate.

In the title compound, C(13)H(9)N(3)O(2)·H(2)O, the dihedral angle between the aromatic rings is 10.7 (4)° and an intra-molecular N-H⋯O hydrogen bond occurs. In the crystal, the components are linked by N-H⋯O, O-H⋯N and O-H⋯O hydrogen bonds.

1-[3-(2-Nitro-phen-yl)-5-phenyl-2-pyrazolin-1-yl]ethanone.

The title compound, C(17)H(15)N(3)O(3), was prepared from 1-(2-nitro-phen-yl)-3-phenyl-prop-2-en-1-one and hydrazine. The dihedral angle between the benzene and phenyl rings is 74.55 (2)°.

Ethyl 3-(4-methyl-benzyl-idene)carbazate.

There are two mol-ecules in the asymmetric unit of the title compound, C(11)H(14)N(2)O(2), which have similar conformations. In the crystal, the mol-ecules are linked by N-H⋯O hydrogen bonds, generating C(4) chains propagating in [001].

N'-(2-Fluoro-benzyl-idene)acetohydrazide.

The title compound, C(9)H(9)FN(2)O, was prepared by the reaction between 2-fluoro-benzophenone and acetohydrazide. In the crystal structure, inversion dimers linked by pairs of N-H⋯O hydrogen bonds occur, generating R(2) (2)(8) loops.

5,6-Diphenyl-3-(3-pyrid-yl)-1,2,4-triazine.

In the mol-ecule of the title compound, C(20)H(14)N(4), the triazine ring is attached to two phenyl rings and one pyridine ring. In the crystal, mol-ecules are linked by inter-molecular C-H⋯N hydrogen bonds. The crystal packing is also stabilized by C-H⋯π inter-actions.

2,7-Dibromo-9,9-bis-[(pyridin-1-ium-4-yl)meth-yl]fluorene dinitrate.

In the title compound, C(25)H(20)Br(2)N(2) (2+)·2NO(3) (-), the cation lies on a twofold rotation axis which imposes disorder of the dibromo-fluorene unit. In addition, the unique nitrate anion is disordered over two general sites of equal occupancy. The crystal structure is stabilized by inter-molecular N-H⋯O hydrogen bonds.

Bis(2,2'-bipyrid-yl)(dichloro-acetato)copper(II) dichloro-acetate dihydrate.

In the title compound, [Cu(C(2)HCl(2)O(2))(C(10)H(8)N(2))(2)](C(2)HCl(2)O(2))·2H(2)O, the Cu(II) ion is bonded to two N,N'-bidentate 2,2'-bipyridyl ligands and one O-monodentate 2,2-dichloro-acetate anion in a distorted CuON(4) trigonal-bipyramidal geometry, with the O atom occupying an equatorial site. In the crystal, the components are linked by O-H⋯O and O-H⋯Cl hydrogen bonds.

N'-(4-Cyano-benzyl-idene)thio-phene-2-carbohydrazide.

The title compound, C(13)H(9)N(3)OS, was prepared by the reaction of thio-phene-2-carbohydrazide and 4-formyl-benzonitrile. The dihedral angle between the benzene and thio-phene rings is 11.9 (1)°.

Methyl 3-(4-methyl-benzyl-idene)carbazate.

The title compound, C(10)H(12)N(2)O(2), was prepared by the reaction of methyl carbazate and 4-methyl-benzaldehyde. The dihedral angle between the benzene ring and the carbazate fragment is 20.86 (10)°.

(E)-N'-(4-Methoxy-benzyl-idene)thio-phene-2-carbohydrazide.

In the title compound, C(13)H(12)N(2)O(2)S, the dihedral angle between the aromatic rings is 15.20 (11)°. In the crystal, inversion dimers linked by pairs of N-H⋯O hydrogen bonds generate R(2) (2)(8) loops.

4-Ethyl-1-(4-methyl-benzyl-idene)thio-semicarbazide.

In the title compound, C(11)H(15)N(3)S, an intra-molecular N-H⋯N hydrogen bond generates an S(5) ring. In the crystal, inversion dimers linked by pairs of N-H⋯S bonds occur, generating an R(2) (2)(8) loop.

N'-(4-Methyl-benzyl-idene)thio-phene-2-carbohydrazide.

In the title compound, C(13)H(12)N(2)OS, the dihedral angle between the aromatic rings is 14.84 (17)°. In the crystal, inversion dimers linked by pairs of N-H⋯O hydrogen bonds generate R(2) (2)(8) loops.