Targeting Neutrophils in Severe Asthma via Siglec-9.

Severe asthma comprises only 5% of patients with asthma, but the burden it brings to the social health system accounts for more than half of all asthmatics. Clinical evidence shows that severe asthma is often linked to the recruitment and activation of neutrophils in the airways. However, the underlying molecular and immunological mechanisms of neutrophilia in severe asthma are not clear and currently available drugs exert only limited effects on neutrophilic inflammation.

Use of serological and mucosal immune responses to Mycoplasma hyopneumoniae antigens P97R1, P46 and P36 in the diagnosis of infection.

Currently available ELISAs used to diagnose Mycoplasma hyopneumoniae infection in pigs have high specificity but low sensitivity. To develop more sensitive assays, the kinetics of specific serum IgG and respiratory mucosal sIgA responses against three M. hyopneumoniae antigens, namely, P97R1 (an adhesin protein), P46 (a membrane protein), and P36 (a cytosolic protein), were characterised over 133 days following experimental infection.

Development of a loop-mediated isothermal amplification assay for rapid detection of Mycoplasma hyorhinis.

Background: To establish a method for sensitive and rapid diagnosis of Mycoplasma hyorhinis in clinical specimens, a simple, sensitive loop-mediated isothermal amplification (LAMP) assay was designed and evaluated.

Methods: Three sets of four special primers, recognizing distinct sequences of the target, were designed for sensitive, specific amplification of nucleic acid under isothermal conditions. The LAMP assay was carried out using 35 clinical specimens of bronchoalveolar lavage fluid (BALF) from pigs.

Development and validation of an attenuated Mycoplasma hyopneumoniae aerosol vaccine.

Mycoplasma hyopneumoniae (M. hyopneumoniae) causes a chronic respiratory disease with high morbidity and low mortality in swine, and has been presented as a major cause of growth retardation in the swine industry. Aerosol vaccination presents a needle free, high throughput, and efficient platform for vaccine delivery, and has been widely applied in poultry vaccination.

[Shoot type morphology and growth characteristics of winter wheat sown at different dates].

Aiming at the delayed sowing of winter wheat induced by the drought and water logging often occurred in Huanghuai Plains of China, six sowing dates (15 October, normal sowing; 30 October, moderate delay; 15 November, delay; 30 November, seriously delay; 15 February, early spring sowing; and 1 March, spring sowing) were designed to investigate the effects of different sowing dates on the shoot type morphology and growth characteristics of winter wheat. With the delay of sowing date, the winter wheat grew and developed faster, and the growing period of the wheat sown in early spring and spring was 115-130 days shorter than that with normal sowing. As compared with those of the wheat with normal sowing, the shoot height, spike number per unit area, and productive spikelets per unit ear of the wheat sown delayed had a decrease, leaf position and canopy moved down, and leaf area reduced.