Clathrin-mediated endocytosis is required for ANE 30-100K-induced autophagy.

Background: We identified an autophagy-inducing areca nut (AN) ingredient (AIAI) in the 30-100 kDa fraction of AN extract (ANE 30-100K). This study was to analyze the role of endocytosis in ANE 30-100K-induced autophagy.

Methods: We used benzyl alcohol, dynasore, and shRNA of clathrin and dynamin to assess whether ANE 30-100K-induced cytotoxicity and accumulation of microtubule-associated protein 1 light chain 3 (LC3)-II were affected in oral (OECM-1) and esophageal (CE81T/VGH) carcinoma cells.

Cost-Effective Kernel Ridge Regression Implementation for Keystroke-Based Active Authentication System.

In this paper, a fast kernel ridge regression (KRR) learning algorithm is adopted with ( ) training cost for large-scale active authentication system. A truncated Gaussian radial basis function (TRBF) kernel is also implemented to provide better cost-performance tradeoff. The fast-KRR algorithm along with the TRBF kernel offers computational advantages over the traditional support vector machine (SVM) with Gaussian-RBF kernel while preserving the error rate performance.