Screening of the PA14NR Transposon Mutant Library Identifies Genes Involved in Resistance to Bacteriophage Infection in .

Multidrug-resistant infections pose a serious public health threat due to the rise in antimicrobial resistance. Phage therapy has emerged as a promising alternative. However, has evolved various mechanisms to thwart phage attacks, making it crucial to decipher these resistance mechanisms to develop effective therapeutic strategies.

[Analysis of pregnancy outcomes, disease progression, and risk factors in patients with undifferentiated connective tissue disease].

Objective: To investigate the fetal and maternal outcomes, risk factors of disease progression and adverse pregnancy outcomes (APOs) in patients with undifferentiated connective tissue disease (UCTD).

Methods: This retrospective study described the outcomes of 106 pregnancies in patients with UCTD. The patients were divided into APOs group (=53) and non-APOs group (=53).

An effective hybrid feature selection using entropy weight method for automatic sleep staging.

. Sleep staging is the basis for sleep quality assessment and diagnosis of sleep-related disorders. In response to the inadequacy of traditional manual judgement of sleep stages, using machine learning techniques for automatic sleep staging has become a hot topic.

Corrected and Republished from: "Isolation and Characterization of the First Freshwater Cyanophage Infecting ".

Cyanobacteria are the major primary producers in both freshwater and marine environments. However, the majority of freshwater cyanophages remain unknown due to the limited number of cyanophage isolates. In this study, we present a novel lytic freshwater cyanophage, PA-SR01, which was isolated from the Singapore Serangoon Reservoir.

A nanobody-based molecular toolkit for ubiquitin-proteasome system explores the main role of survivin subcellular localization.

Targeted protein degradation is a powerful tool for determining the function of specific proteins nowadays. Survivin is the smallest member of the inhibitor of the apoptosis protein (IAP) family. It exists in the cytoplasm and nucleus of cells, but the exact function of survivin in different subcellular locations retained unclear updates due to the lack of effective and simple technical means.

Multimodal Warnings Design for In-Vehicle Robots under Driving Safety Scenarios.

In case of dangerous driving, the in-vehicle robot can provide multimodal warnings to help the driver correct the wrong operation, so the impact of the warning signal itself on driving safety needs to be reduced. This study investigates the design of multimodal warnings for in-vehicle robots under driving safety warning scenarios. Based on transparency theory, this study addressed the content and timing of visual and auditory modality warning outputs and discussed the effects of different robot speech and facial expressions on driving safety.

TJZYF Improves Endometrial Receptivity through Regulating VEGF and PI3K/AKT Signaling Pathway.

The endometrium receptivity was impaired by controlled ovarian hyperstimulation (COH), which would then lead to fertility issues and increased abortion clinically. In the present study, to explore the effectiveness of Tiaojing Zhuyun Formula (TJZYF) in improving endometrial receptivity of COH rats and the possible active ingredients and mechanisms, an approach of network pharmacology was performed and a COH animal model was established. As analyzed, stigmasterol and quercetin may be the active ingredients of TJZYF on improving endometrial receptivity and positive regulation of ion transport, the cytokine-mediated signaling pathway, and endocrine process, and vascular endothelial growth factor receptor signaling pathway may be involved.

Adaptive growth and acidogenic fermentation performance of haloalkaliphilic bacterial communities enriched from biofilms colonising strongly alkaline and saline bauxite residue.

The present study aimed to characterise the adaptive growth and acidogenic fermentation performance of haloalkaliphilic bacteria sourced from field biofilms colonising seawater-treated bauxite residue, under moderate and extremely alkaline pH conditions (8.5 to 10.8) and coupled saline (EC ≈ 50 mS/cm) conditions.

A comparative study of flow cytometry-sorted communities and shotgun viral metagenomics in a Singapore municipal wastewater treatment plant.

Traditional or "bulk" viral enrichment and amplification methods used in viral metagenomics introduce unavoidable bias in viral diversity. This bias is due to shortcomings in existing viral enrichment methods and overshadowing by the more abundant viral populations. To reduce the complexity and improve the resolution of viral diversity, we developed a strategy coupling fluorescence-activated cell sorting (FACS) with random amplification and compared this to bulk metagenomics.

(Champ. ex Benth.) C. C. Huang and (Champ. ex Benth.) Swingle as Independent Species: Evidence From Morphology and Molecular Systematics and Taxonomic Revision of (Rutaceae).

Recently, the systematic status of Swingle and its taxonomy has attracted much attention. Flora of China incorporates into Linn. and treats all species of the traditional as one species, namely (Thunb.

Design of Proactive Interaction for In-Vehicle Robots Based on Transparency.

Based on the transparency theory, this study investigates the appropriate amount of transparency information expressed by the in-vehicle robot under two channels of voice and visual in a proactive interaction scenario. The experiments are to test and evaluate different transparency levels and combinations of information in different channels of the in-vehicle robot, based on a driving simulator to collect subjective and objective data, which focuses on users' safety, usability, trust, and emotion dimensions under driving conditions. The results show that appropriate transparency expression is able to improve drivers' driving control and subjective evaluation and that drivers need a different amount of transparency information in different types of tasks.

Grazing Effects of Soil Fauna on White-Rot Fungi: Biomass, Enzyme Production and Litter Decomposition Ability.

Soil invertebrates and microorganisms are two major drivers of litter decomposition. Even though the importance of invertebrates and microorganisms in biogeochemical soil cycles and soil food webs has been studied, the effects of invertebrates on fungi are not well understood compared to other organisms. In this work, we investigated the effects of soil invertebrates on fungi as a factor that cannot be ignored in the study of nutrient cycling.

Electroacupuncture improves endometrial receptivity through miRNA-223-3p-mediated regulation of leukemia inhibitory factor / signal transducer and activator of transcription 3 signaling pathway.

Ovulation-inducing drugs such as endogenous steroids could reduce endometrial receptivity during the implantation window, resulting in lower clinical pregnancy rates and higher miscarriage rates. The present study employed electroacupuncture therapy along with different frequencies on elevating impaired endometrial receptivity to elucidate the mechanism therein. The rats were split up into seven groups of normal, model, low-frequency electroacupuncture (LF-EA), high-frequency electroacupuncture (HF-EA), LF-EA+anti-miRNA, HF-EA+anti-miRNA, and anti-miRNA.

Pre-culturing soil microbial inoculum in plant residues enhanced the resilience of tolerant bacteria and bioneutralization efficacy in alkaline bauxite residues.

Bioneutralization of alkaline bauxite residues (BR) may be achieved through in situ organic acids produced from fermentative decomposition of carbohydrates-rich organic matters (e.g., plant residues), which are driven by organophilic and heterotrophic prokaryotes tolerant of extremely saline and alkaline conditions.

TNFRSF11B Suppresses Memory CD4+ T Cell Infiltration in the Colon Cancer Microenvironment: A Multiomics Integrative Analysis.

Background: Colorectal cancer is a lethal cancer worldwide. Due to the low tumor mutation burden and low proportion of tumor-infiltrating lymphocytes in the microenvironment of most patients, innovative immunotherapeutic approaches need to be identified.

Methods: Using the TCGA-COAD dataset (n = 514), we identified TNFRSF11B as a prognostic factor of colon cancer.

High-frequency electroacupuncture improves endometrial receptivity via regulating cell adhesion molecules and leukemia inhibitory factor / signal transducer and activator of transcription signaling pathway.

Controlled ovarian hyperstimulation (COH) impairs the endometrium receptivity during the implantation window, resulting in a lower clinical pregnancy rate and a higher abortion rate. Our study explored the effect of electroacupuncture on the endometrial receptivity of COH rats. Female rats were randomly divided into normal treatment (Normal), model treatment (Model), low-frequency electroacupuncture treatment (LF-EA) and high-frequency electroacupuncture treatment (HF-EA).

Characteristics of prokaryotic and fungal communities emerged in eco-engineered waste rock - Eucalyptus open woodlands at Ranger uranium mine.

Diverse prokaryotic and fungal communities in soil and litters are the structural basis for driving tree litter decomposition and inherent nutrient cycling in infertile Eucalyptus open woodlands. The present investigation characterized the composition and co-occurrence network of prokaryotic and fungal communities in litter and surface soil layers in 9-year old revegetated trial landforms at Ranger uranium mine, Northern Territory, Australia. The revegetated landforms consisted of soil-subsystems engineered from waste rocks and plant-subsystems of young, novel and native Eucalyptus open woodlands.

Hyperin Alleviates Triptolide-Induced Ovarian Granulosa Cell Injury by Regulating AKT/TSC1/mTORC1 Signaling.

Premature ovarian insufficiency (POI) is characterized by the loss of ovarian function before 40 years of age and affects approximately 1% of women worldwide. is a traditional Miao (a Chinese ethnic minority) medicine that improves ovarian function and follicular development. In the present study, we aimed to investigate the effect of active ingredients of on POI and determine underlying mechanisms.

Chemodiversity of Dissolved Organic Matter and Its Molecular Changes Driven by Rhizosphere Activities in Fe Ore Tailings Undergoing Eco-Engineered Pedogenesis.

Dissolved organic matter (DOM) plays an important role in soil structure and biogeochemical function development, which are fundamental for the eco-engineering of tailings-soil formation to underpin sustainable tailings rehabilitation. In the present study, we have characterized the DOM composition and its molecular changes in an alkaline Fe ore tailing primed with organic matter (OM) amendment and plant colonization. The results demonstrated that microbial OM decomposition dramatically increased DOM richness and average molecular weight, as well as its degree of unsaturation, aromaticity, and oxidation in the tailings.

Robot Transparency and Anthropomorphic Attribute Effects on Human-Robot Interactions.

Anthropomorphic robots need to maintain effective and emotive communication with humans as automotive agents to establish and maintain effective human-robot performances and positive human experiences. Previous research has shown that the characteristics of robot communication positively affect human-robot interaction outcomes such as usability, trust, workload, and performance. In this study, we investigated the characteristics of transparency and anthropomorphism in robotic dual-channel communication, encompassing the voice channel (low or high, increasing the amount of information provided by textual information) and the visual channel (low or high, increasing the amount of information provided by expressive information).