Sensitive subcellular scale and real-time detection of hydrogen peroxide by a W-doped Pt microelectrode.

A W-doped Pt modified graphene oxide (Pt-W-GO) electrochemical microelectrode was developed to detect hydrogen peroxide (HO) in real time at a subcellular scale. Interestingly, results showed that the concentration of HO in the nucleus of HeLa cells was 2.68 times and 0.

Integrated molybdenum single atom array sensors with multichannels for nitrite detection in foods.

Nitrite (NO) is present in a variety of foods, but the excessive intake of NO can indirectly lead to carcinogenic, teratogenic, mutagenicity and other risks to the human body. Therefore, the detection of NO is crucial for maintaining human health. In this study, an integrated array sensor for NO detection is developed based on molybdenum single atom material (IMS) using high-resolution electrohydrodynamic (EHD) printing technology.

Machine Learning-Assistant Colorimetric Sensor Arrays for Intelligent and Rapid Diagnosis of Urinary Tract Infection.

Urinary tract infections (UTIs), which can lead to pyelonephritis, urosepsis, and even death, are among the most prevalent infectious diseases worldwide, with a notable increase in treatment costs due to the emergence of drug-resistant pathogens. Current diagnostic strategies for UTIs, such as urine culture and flow cytometry, require time-consuming protocols and expensive equipment. We present here a machine learning-assisted colorimetric sensor array based on recognition of ligand-functionalized Fe single-atom nanozymes (SANs) for the identification of microorganisms at the order, genus, and species levels.

Nickel Single-Atom Catalyst Boosts Electrochemiluminescence of Graphitic Carbon Nitride for Sensitive Detection of HBV DNA.

Owing to excellent catalytic activity, single-atom catalysts (SACs) have recently attracted considerable research interest in the electrochemiluminescence (ECL) field. However, the applications of SACs are mostly limited to conventional luminol ECL system. Hence, it is necessary to explore the application of SACs in more ECL systems.

A single-atom cobalt integrated flexible sensor for simultaneous detection of dihydroxybenzene isomers.

Simultaneous detection of dihydroxybenzene isomers including hydroquinone (HQ), catechol (CC), and resorcinol (RS) is significant for water quality control as they are highly toxic and often coexist. However, it is a great challenge to realize their accurate and simultaneous detection due to their similarity in structure and properties. Herein, an electrochemical flexible strip with single-atom cobalt (SA-Co/NG) was constructed through high-resolution electrohydrodynamic (EHD) printing for dihydroxybenzene isomer's simultaneous detection.

Integrated Biochip-Electronic System with Single-Atom Nanozyme for Analysis of Nitric Oxide.

Nitric oxide (NO) exhibits a crucial role in various versatile and distinct physiological functions. Hence, its real-time sensing is highly important. Herein, we developed an integrated nanoelectronic system comprising a cobalt single-atom nanozyme (Co-SAE) chip array sensor and an electronic signal processing module (IND) for both and multichannel qualifying of NO in normal and tumor-bearing mice.

Unique BiFeO/g-CN mushroom heterojunction with photocatalytic antibacterial and wound therapeutic activity.

Bacterial infections have become a major problem threatening public health, and it is of great significance to treat wound infections in biological systems caused by bacteria. However, traditionally used bacteriostatic agents usually cause additional pollution. Herein a mushroom-shaped clean and Green BiFeO/g-CN composite is employed for the first time for photocatalytic antibacterial activity and for the further promotion of wound healing.

Single-Atom Cobalt-Based Electrochemical Biomimetic Uric Acid Sensor with Wide Linear Range and Ultralow Detection Limit.

Uric acid (UA) detection is essential in diagnosis of arthritis, preeclampsia, renal disorder, and cardiovascular diseases, but it is very challenging to realize the required wide detection range and low detection limit. We present here a single-atom catalyst consisting of Co atoms coordinated by an average of 3.4 N atoms on an N-doped graphene matrix (A-Co-NG) to build an electrochemical biomimetic sensor for UA detection.

Recent advances in single atom catalysts for the electrochemical carbon dioxide reduction reaction.

The electrochemical carbon dioxide reduction reaction (CORR) offers a promising solution to mitigate carbon emission and at the same time generate valuable carbonaceous chemicals/fuels. Single atom catalysts (SACs) are encouraging to catalyze the electrochemical CORR due to the tunable electronic structure of the central metal atoms, which can regulate the adsorption energy of reactants and reaction intermediates. Moreover, SACs form a bridge between homogeneous and heterogeneous catalysts, providing an ideal platform to explore the reaction mechanism of electrochemical reactions.

Single-Atom Ruthenium Biomimetic Enzyme for Simultaneous Electrochemical Detection of Dopamine and Uric Acid.

Single-atom catalysts have attracted numerous attention due to the high utilization of metallic atoms, abundant active sites, and highly catalytic activities. Herein, a single-atom ruthenium biomimetic enzyme (Ru-Ala-CN) is prepared by dispersing Ru atoms on a carbon nitride support for the simultaneous electrochemical detection of dopamine (DA) and uric acid (UA), which are coexisting important biological molecules involving in many physiological and pathological aspects. The morphology and elemental states of the single-atom Ru catalyst are studied by transmission electron microscopy, energy dispersive X-ray elemental mapping, high-angle annular dark field-scanning transmission electron microscopy, and high-resolution X-ray photoelectron spectroscopy.

Enhanced electrochemiluminescence from reduced graphene oxide-CdTe quantum dots for highly selective determination of copper ion.

An electrochemiluminescence (ECL) sensor based on reduced graphene oxide-CdTe quantum dots (RGO-CdTe QDs) composites for detecting copper ion (Cu ) was proposed. The ECL behaviours of the RGO-CdTe QD modified electrode were investigated with H O as the co-reactant. Quantitative detection of Cu was realized as Cu could effectively quench the ECL signal of the RGO-CdTe QDs.

Involvement of superoxide and nitric oxide in BRAF(V600E) inhibitor PLX4032-induced growth inhibition of melanoma cells.

The BRAF(V600E) inhibitor PLX4032 (Vemurafenib) is an FDA-approved new drug for the treatment of metastatic melanomas, which specifically inhibits the RAS/MEK/ERK signaling pathway to control cell proliferation and adhesion. However, no study has been carried out to investigate the role of intracellular oxidative balance in PLX4032-induced tumor growth inhibition. Herein, for the first time, superoxide (O2˙(-)) and nitric oxide (NO) generated from PLX4032-challenged melanoma cells were monitored using electrochemical sensors and conventional fluorescein staining techniques.

The construction of glucose biosensor based on platinum nanoclusters-multiwalled carbon nanotubes nanocomposites.

One-step synthesis method was proposed to obtain the nanocomposites of platinum nanoclusters and multiwalled carbon nanotubes (PtNCs-MWNTs), which were used as a novel immobilization matrix for the enzyme to fabricate glucose biosensor. The fabrication process of the biosensor was characterized by cyclic voltammetry, electrochemical impedance spectroscopy, atomic force microscopy and scanning electron microscope. Due to the favorable characteristic of PtNCs-MWNTs nanocomposites, the biosensor exhibited good characteristics, such as wide linear range (3.