[Immune response of purified Plasmodium falciparum antigen on human peripheral blood T lymphocytes in vitro].

Objective: To explore the effects of the purified Plasmodiumfalciparum (P. f) antigen on the T lymphocytes of the peripheral blood of healthy people in vitro.

Methods: The peripheral blood mononuclear cells (PBMCs) isolated from healthy donators were cultured with purified P.

Catheter-related Candida bloodstream infection in intensive care unit patients: a subgroup analysis of the China-SCAN study.

Background: In patients hospitalized in intensive care units (ICU), Candida infections are associated with increased morbidity, mortality and costs. However, previous studies reported confused risk factors for catheter-related Candida bloodstream infection (CRCBSI). The objective was to describe the risk factors, microbiology, management and outcomes of CRCBSI in the China-SCAN population.

Postpolymerization of a fluorinated and reactive poly(aryl ether): an efficient way to balance the solubility and solvent resistance of the polymer.

A new fluorinated poly(aryl ether) with reactive benzocyclobutene groups as the side chain was successfully synthesized. This polymer showed a number-average molecular weight (Mn) of 200,000 and had good solubility and film-forming ability. After being postpolymerized at high temperature (>200 °C), the polymer film converted to a cross-linked network structure, which was insoluble in the common organic solvents.

[Research progress on Plasmodium vivax chloroquine resistance].

Malaria remains a serious public health problem, especially in developing countries. With the deepening of the understanding of vivax malaria, Plasmodium vivax is also attracting more and more attention. An effective drug treatment is the foundation of controlling or even eliminating malaria.

[Plasmodium vivax specific peptides prediction and screening based on repetitive protein sequences and linear B cell epitope].

Objective: To establish a method based on repetitive protein sequences and linear B cell epitope to predict and screen specific peptides of Plasmodium vivax.

Methods: A P. vivax protein sequence database was reconstructed based on PlasmoDB data, and a customized software for searching of repetitive sequences was used to count the repetition times of each 16 aa peptide in the whole database, and the highly repetitive peptides were chosen to predict the potential linear B cell epitopes.

[Construction and identification of recombinant Mycobacterium smegmatis vaccine expressing Cysticercus cellulosae cC1 antigen].

Objective: To construct recombinant Mycobacterium smegmatis vaccine expressing Cysticercus cellulosae cC1 antigen.

Methods: The recombinant pET28a-cC1 plasmid was extracted and double digested by Xho I and BamH I restriction enzymes, and shuttle plasmid pMV261 was extracted and double digested by Hind III and BamH I restriction enzymes. Both fragments were modified by Klenow fragment to form blunt end, then the large fragments of cC1 and pMV261 plasmid were purified and ligated by T4 ligase enzyme.

Fiber lasers and their applications [Invited].

Fiber lasers have seen progressive developments in terms of spectral coverage and linewidth, output power, pulse energy, and ultrashort pulse width since the first demonstration of a glass fiber laser in 1964. Their applications have extended into a variety of fields accordingly. In this paper, the milestones of glass fiber laser development are briefly reviewed and recent advances of high-power continuous wave, Q-switched, mode-locked, and single-frequency fiber lasers in the 1, 1.

Role of AMPK α in skeletal muscle glycometabolism regulation and adaptation in relation to sepsis.

Background: AMP-activated protein kinase (AMPK) and the translocation of glucose transporter 4 (GLUT4) protein always involve disturbance of carbohydrate metabolism.

Objective: To determine whether the change of blood glucose in the early stage of septic rat is associated with the alteration of AMPKα protein expression and GLUT4 protein translocation expression.

Methods: Animal models of sepsis were induced by tail vein injection of LPS in Wistar rats.

[Remote intelligent Brunnstrom assessment system for upper limb rehabilitation for post-stroke based on extreme learning machine].

In order to realize an individualized and specialized rehabilitation assessment of remoteness and intelligence, we set up a remote intelligent assessment system of upper limb movement function of post-stroke patients during rehabilitation. By using the remote rehabilitation training sensors and client data sampling software, we collected and uploaded the gesture data from a patient's forearm and upper arm during rehabilitation training to database of the server. Then a remote intelligent assessment system, which had been developed based on the extreme learning machine (ELM) algorithm and Brunnstrom stage assessment standard, was used to evaluate the gesture data.

[Sequence homology analysis on lactate dehydrogenase (LDH) of Plasmodium vivax Anhui isolates].

DNA in dried blood spots of 39 vivax malaria patients (2009-2010) from Anhui (Bengbu urban district and counties of Wuhe, Huaiyuan, Mengcheng and Lixin) was extracted. The Plasmodium vivax LDH (PvLDH) gene was amplified, cloned and sequenced. The sequences were subjected to NCBI Blast program.

[Role of CD8+ T cells in the tumor growth delay induced by Toxoplasma gondii excreted-secreted antigen in B16F10 mouse melanoma model].

Objective: To observe the role of CD8+ T cells in the tumor growth delay induced by Toxoplasma gondii excreted-secreted antigens (TgESA) in B16FI0 mouse melanoma model in the early stage.

Methods: TgESA were prepared by incubating T. gondii tachyzoites for 12 h in vitro.

[Effect of PvMSP1 on differentiation, maturation and function of dendritic cells].

Objective: To investigate the effects of Plasmodium vivax merozoite surface protein 1 (PvMSP1) on differentiation, maturation and function of dendritic cells (DC) and the mechanisms of PvMSP1 on the activation of DC via toll like receptors (TLR).

Methods: DCs were incubated with different doses of PvMSP1 (1.0, 10.

Interconnected growing self-organizing maps for auditory and semantic acquisition modeling.

Based on the incremental nature of knowledge acquisition, in this study we propose a growing self-organizing neural network approach for modeling the acquisition of auditory and semantic categories. We introduce an Interconnected Growing Self-Organizing Maps (I-GSOM) algorithm, which takes associations between auditory information and semantic information into consideration, in this paper. Direct phonetic-semantic association is simulated in order to model the language acquisition in early phases, such as the babbling and imitation stages, in which no phonological representations exist.

Congenital malaria in China.

Background: Congenital malaria, in which infants are directly infected with malaria parasites from their mother prior to or during birth, is a potentially life-threatening condition that occurs at relatively low rates in malaria-endemic regions. It is recognized as a serious problem in Plasmodium falciparum-endemic sub-Saharan Africa, where recent data suggests that it is more common than previously believed. In such regions where malaria transmission is high, neonates may be protected from disease caused by congenital malaria through the transfer of maternal antibodies against the parasite.

Copper(II)-catalyzed enantioselective hydrosilylation of halo-substituted alkyl aryl and heteroaryl ketones: asymmetric synthesis of (R)-fluoxetine and (S)-duloxetine.

A set of reaction conditions has been established to facilitate the non-precious copper-catalyzed enantioselective hydrosilylation of a number of structurally diverse β-, γ- or ε-halo-substituted alkyl aryl ketones and α-, β- or γ-halo-substituted alkyl heteroaryl ketones under air to afford a broad spectrum of halo alcohols in high yields and good to excellent enantioselectivities (up to 99% ee). The developed procedure has been successfully applied to the asymmetric synthesis of antidepressant drugs (R)-fluoxetine and (S)-duloxetine, which highlighted its synthetic utility.

230 W average-power all-fiber-based actively Q-switched fiber master oscillator-power amplifier.

We report a high-power all-fiber format pulsed laser source at ~1064 nm in a master oscillator-power amplifier configuration. The seed source is an acousto-optic Q-switched fiber laser with a varied pulse duration and repetition rate. The output power of the oscillator is ~1 W, and two double-cladding Yb-doped fiber amplifiers were used to boost the average power of the seed.

[Deltamethrin resistance, metabolic detoxification enzyme and kdr mutation in Anopheles sinensis in region along Huaihe River in Anhui Province].

Objective: To understand the deltamethrin resistance level, GTS and P450 metabolic detoxification enzyme activities, and mutations in the para-type sodium channel gene that confers knockdown resistance (kdr) in Anopheles sinensis mosquitoes from malaria endemic areas along the Huaihe River in Anhui Province, China.

Methods: An. sinensis adult mosquitoes were collected in Lilou, Mohekou and Tuohu townships of Bengbu City, Anhui Province from August to September, 2011.

Invasive candidiasis in intensive care units in China: in vitro antifungal susceptibility in the China-SCAN study.

Objectives: The objectives of this study were to determine species distribution and in vitro antifungal susceptibility of Candida isolates identified in the multicentre China-SCAN study of invasive Candida infection (ICI) in intensive care units (ICUs) across China.

Methods: Candida isolates from patients in the China-SCAN study with documented ICI were evaluated by a central laboratory. Species were identified using chromogenic culture media or the API 20C AUX kit.

[Changes of pyrethroid resistance and P450 monooxygenase activity with age in Anopheles sinensis in Huainan City, Anhui Province, China].

Objective: To study the effect of mosquito age on the pyrethroid resistance and P450 monooxygenase activity in Anopheles sinensis in Huainan City, Anhui Province, China, as well as the molecular mechanism of pyrethroid resistance.

Methods: Anopheles mosquito larvae were collected in fields, and were emerged to adults. Then the pyrethroid resistance and P450 monooxygenase activity of female mosquitoes were detected 1, 3, 6, 9, 12, 15 d and 20 d after emergence.

A directed network analysis of heterospecific pollen transfer in a biodiverse community.

Community studies have shown that plant species are often pollinated by multiple pollinators; however, networks of heterospecific pollen transfer (HPT) in natural communities remain largely unexplored. We analyzed pollen deposition on stigmas of 57 flowering species to build a picture of plant-plant interactions via HPT in a biodiverse alpine meadow in southwest China. Plant species were categorized as pollen donors or recipients by their link numbers and link qualities.

Non-porous low-k dielectric films based on a new structural amorphous fluoropolymer.

A non-porous and amorphous fluoropolymer PFN with low dielectric constant of 2.33 and dielectric loss less than 1.2 × 10(-3) is reported here.