Retropupillary sulcus gas migration after retinal detachment repair surgery.

Purpose: To elucidate etiology and management of retropupillary sulcus migration of intravitreal gas after uneventful retinal detachment repair surgery.

Methods: 70 year old Caucasian man presented with a temporal macula-off rhegmatogenous retinal detachment. 25-gauge (25G) pars plana vitrectomy was performed with cryopexy to retinal tear and 12% C3F8 gas tamponade under sub-Tenon's anaesthesia.

A double boronic acid affinity "sandwich" SERS biosensor based on magnetic boronic acid controllable-oriented imprinting for high-affinity biomimetic specific recognition and rapid detection of target glycoproteins.

Transferrin (TRF), recognized as a glycoprotein clinical biomarker and therapeutic target, has its concentration applicable for disease diagnosis and treatment monitoring. Consequently, this study developed boronic acid affinity magnetic surface molecularly imprinted polymers (B-MMIPs) with pH-responsitivity as the "capture probe" for TRF, which have high affinity similar to antibodies, with a dissociation constant of (3.82 ± 0.

APC/C-regulated CPT1C promotes tumor progression by upregulating the energy supply and accelerating the G1/S transition.

Background: In addition to functioning as a precise monitoring mechanism in cell cycle, the anaphase-promoting complex/cyclosome (APC/C) is reported to be involved in regulating multiple metabolic processes by facilitating the ubiquitin-mediated degradation of key enzymes. Fatty acid oxidation is a metabolic pathway utilized by tumor cells that is crucial for malignant progression; however, its association with APC/C remains to be explored.

Methods: Cell cycle synchronization, immunoblotting, and propidium iodide staining were performed to investigate the carnitine palmitoyltransferase 1 C (CPT1C) expression manner.

FPBA-modified magnetic nanoparticles combined with Au@AgNPs for label-free SERS detection of foodborne pathogens in milk.

We report a low-cost and highly sensitive label-free SERS biosensor for pathogen detection. Herein, this study prepared 4-formylphenylboric acid (FPBA) functionalized magnetic nanoparticles to adsorb pathogenic bacteria through boric acid affinity principle, and used aptamer modified Au@AgNPs as SERS substrate to specifically combine with pathogenic bacteria to form a sandwich structure. The pathogenic bacteria were detected by portable Raman spectrometer for SERS detection, and the fingerprint signals of pathogenic bacteria were analyzed by principal component analysis (PCA) to achieve the purpose of classification and identification of pathogenic bacteria.

Combining multisite functionalized magnetic nanomaterials with interference-free SERS nanotags for multi-target sepsis biomarker detection.

Surface-enhanced Raman scattering (SERS) is an ultra-sensitive vibration spectroscopy technology, with the advantages of multi-index and non-destructive quantitative detection, has attracted much attention in the joint detection of biomarkers. A novel SERS biosensor with multisite capture and interference-free quantification was designed for the joint detection of the sepsis biomarker interleukin-6 (IL-6) and procalcitonin (PCT). This biosensor had two interference-free core-shell SERS probes with highly efficient electromagnetic enhancement and a multisite functionalized magnetic nanomaterial with high adsorption capacity.

High-sensitivity biosensor based on SERS integrated with dendrimer-assisted boronic acid-functionalized magnetic nanoparticles for IL-6 detection in human serum.

High-sensitivity quantitative analysis of sepsis disease markers in circulating blood is essential for sepsis early diagnosis, rapid stratification, and interventional treatment. Herein, a high-sensitivity biosensor combining surface-enhanced Raman spectroscopy (SERS) and functionalized magnetic materials was developed to quantitatively detect interleukin-6 (IL-6), a glycoprotein disease marker closely related to sepsis. First, boronic acid-functionalized magnetic nanomaterials with high adsorption performance were synthesized by utilizing the branched polyethyleneimine to provide many binding sites for boronic acid.

Integration of three non-interfering SERS probes combined with ConA-functionalized magnetic nanoparticles for extraction and detection of multiple foodborne pathogens.

A sandwich-structured SERS biosensor has been constructed for simultaneous detection of multiple pathogenic bacteria, consisting of non-interfering SERS probes for bacterial labeling and ConA-functionalizd magnetic nanoparticles for bacteria extraction. A the preparation method of PP3 SERS probe with high Raman activity is reported for the first time. Since the PP3 SERS probe has a very strong Raman peak at 2081 cm in the "Raman silent region," the mixed SERS probe formed with MP1 and DP2 can meet the needs of multiple foodborne pathogen detection.

Relationships between the Mini-InDel Variants within the Goat Gene and Body Traits.

The cilia- and flagella-associated protein 43 () gene encodes a member of the cilia- and flagellum-associated protein family. Cilia on the cell surface influence intercellular signaling and are involved in biological processes such as osteogenesis and energy metabolism in animals. Previous studies have shown that insertion/deletion (InDel) variants in the gene affect litter size in Shaanbei white cashmere (SBWC) goats, and that litter size and body traits are correlated in this breed.

Detection of genetic variation in bovine gene and its associations with carcass traits.

The biological clock (also known as circadian clock) is closely related to growth and development, metabolism, and diseases in animals. As a part of the circadian clock, the cryptochrome circadian regulator 1 () gene is involved in the regulation of biological processes such as osteogenesis, energy metabolism and cell proliferation, however, few studies have been reported on the relationship between this gene and animal carcass traits. Herein, a total of four insertion/deletion (InDel) loci within the gene were detected in Shandong Black Cattle Genetic Resource (SDBCGR) population ( = 433).

BRANCH RETINAL ARTERY OCCLUSION IN A YOUNG PATIENT AFTER RADIOTHERAPY FOR NASOPHARYNGEAL CARCINOMA BRANCH RETINAL ARTERY OCCLUSION AFTER RADIOTHERAPY.

Purpose: The aim of this study was to report a case of branch retinal artery occlusion in a young patient who received previous neck radiotherapy for nasopharyngeal carcinoma.

Methods: The authors describe an interesting case of a branch retinal artery occlusion in a patient with previous neck radiotherapy for nasopharyngeal carcinoma 14 years ago. The patient was a 49-year-old man, who presented to the retina service in Tan Tock Seng Hospital.

Recent advancements in microfluidic chip biosensor detection of foodborne pathogenic bacteria: a review.

Foodborne diseases caused by pathogenic bacteria pose a serious threat to human health. Early and rapid detection of foodborne pathogens is an urgent task for preventing disease outbreaks. Microfluidic devices are simple, automatic, and portable miniaturized systems.

Application progress of magnetic molecularly imprinted polymers chemical sensors in the detection of biomarkers.

Specific recognition and highly sensitive detection of biomarkers play an essential role in identification, early diagnosis and prevention of many diseases. Magnetic molecularly imprinted polymers (MMIPs) have been widely used to capture biomimetic receptors for targets in various complex matrices due to their superior recognition ability, structural stability, and rapid separation characteristics, which overcome the existing deficiencies of traditional recognition elements such as antibodies, aptamers. The integration of MMIPs as recognition elements with chemical sensors opens new opportunities for the development of advanced analytical devices with improved selectivity and sensitivity, shorter analysis time, and lower cost.

SERS-based boronate affinity biosensor with biomimetic specificity and versatility: Surface-imprinted magnetic polymers as recognition elements to detect glycoproteins.

Glycoproteins are a class of proteins with significant biological functions and clinical implications. Due to glycoproteins' reliability for the quantitative analysis, they have been used as biomarkers and therapeutic targets for disease diagnosis. We propose a sandwich structure-based boronate affinity biosensor that can separate and detect target glycoproteins by magnetic separation and Surface-enhanced Raman scattering (SERS) probes.

Application of lectin-based biosensor technology in the detection of foodborne pathogenic bacteria: a review.

Foodborne diseases caused by pathogenic bacteria pose a serious threat to human health. Early and rapid detection of foodborne pathogens is urgently needed. The use of biosensors to identify and detect pathogenic bacteria has attracted ample attention because of their high sensitivity, near real-time quantification without enrichment, on-site detection, simple operation, and so on.

SERS-based magnetic immunoassay for simultaneous detection of cTnI and H-FABP using core-shell nanotags.

Acute myocardial infarction (AMI) is the single leading cause of worldwide mortality and morbidity. Heart-type fatty acid-binding protein (H-FABP) and cardiac troponin I (cTnI), as biomarkers emerging at different stages of AMI, have complementary advantages in terms of specificity and sensitivity. Therefore, we developed a magnetic immunoassay method based on surface-enhanced Raman scattering (SERS) to detect H-FABP and cTnI simultaneously.

A novel surface-enhanced Raman scattering method for simultaneous detection of ketamine and amphetamine.

As common psychotropic drugs, ketamine (KET) and amphetamine (AMP) are often consumed by drug users at the same time, which seriously threatens people's health. Therefore, the study of simultaneous detection methods for KET and AMP is imperative. In this study, a novel method for the simultaneous detection of KET and AMP in serum was established on the basis of surface-enhanced Raman scattering (SERS).

Toll-Like Receptor 4-Myeloid Differentiation Primary Response Gene 88 Pathway Is Involved in the Inflammatory Development of Polymyositis by Mediating Interferon-γ and Interleukin-17A in Humans and Experimental Autoimmune Myositis Mouse Model.

Objective: Toll-like receptor 4 (TLR4) is one of the key players in the development of many autoimmune diseases. To determine the possible role of TLR4 in polymyositis (PM) development, we collected muscle samples from PM patients and mice subjected to an experimental autoimmune myositis (EAM) model.

Methods: We measured TLR4-MyD88 pathway-related factors, interferon-γ (IFN-γ), and interleukin-17A (IL-17A) in EAM mice and PM patients.

Identification and Validation Novel of VIM-2 Metallo-β-lactamase Tripeptide Inhibitors.

The development of novel inhibitors against metallo-β-lactamase is essential to remedy metallo-β-lactamase mediated bacterial resistance. A recently emerged metallo-β-lactamase, VIM-2, has demonstrated resistance to existing β-lactamase inhibitors in the clinic. In this study, a hybrid virtual screening protocol that combines pharmacophore modeling, molecular docking, and calculation of binding free energy was employed to screen an internal tripeptide database for novel inhibitors against VIM-2.