Directed evolution of diacylglycerol acyltransferases 2 promotes lipids and triglyceride accumulation.

Triacylglycerol (TAG) is a major component of plant-neutral lipids. Diacylglycerol acyltransferase 2 (DGAT2) plays an important role in plant oil accumulation by catalyzing the final step of the Kennedy pathway. In this study, ten DGAT2 sequences were originating from different oil crops into the TAG-deficient yeast strain H1246, to compare their enzyme activity of oil synthesis and filter out potential amino acid residue sites for directed evolution.

Self-Supplying Photovoltaic-Hygroelectric Coupling System Powering Internet of Things Sensors.

The rapid advancement in the Internet of Things (IoT) has prompted a proliferation of sensor applications with increasing focus on harnessing environmental energy sources for powering these sensors. However, owing to the variability inherent in climatic and geographic conditions, a singular approach to environmental energy harvesting often fails to deliver sustainable and reliable power. Hygroelectric technology, leveraging the electrical coupling between nanostructured materials and water to convert moisture-derived energy into electricity, complements the advantages of light energy collection technology.

Sea Urchin-Like NiCo-LDH Hollow Spheres Anchored on 3D Graphene Aerogel for High-Performance Supercapacitors.

To enhance the inherent poor conductivity and low cycling stability of dimetallic layered double hydroxides (LDHs) materials, designing a synergistic effect between EDLC capacitors and pseudocapacitors is an efficient strategy. In this paper, we utilized a solvothermal technique employing Co-glycerate as a precursor to prepare sea urchin-like NiCo-LDH hollow spheres anchored on a 3D graphene aerogel. The unique morphology of these hollow microspheres significantly expand the specific surface area and exposes more active sites, while reducing the volume changes of materials during long-term charging and discharging processes.

Efficient and cold-tolerant moisture-enabled power generator combining ionic diode and ionic hydrogel.

The ionic diode structure has become one of the attractive structures in the field of moisture-based power generation. However, existing devices still suffer from poor moisture trapping, low surface charge, and inefficient ion separation, resulting in low output power. Moreover, water freezes at low temperatures (<0 °C), limiting the ionic diode structure to generate electricity in cold environments.

Bioinspired ion channel receptor based on hygroelectricity for precontact sensing of living organism.

Tactile sensors play an important role in human-machine interaction (HMI). Compared to contact tactile sensing, which leaves physical hardware vulnerable to wear and tear, proximity sensing is better at reacting to remote events before physical contact. The apteronotus albifrons possess ion channel receptors for remote surroundings perception.

Research on Coix seed as a food and medicinal resource, it's chemical components and their pharmacological activities: A review.

Ethnopharmacological Relevance: Coix lacryma-jobi var. ma-yuen (Romanet du Caillaud) Stapf is a plant of the genus Coix in the Gramineae family. Coix seed is cultivated in various regions throughout China.

Prosthetic finger for fingertip tactile sensing flexible chromatic optical waveguides.

Building prosthetics indistinguishable from human limbs to accurately receive and transmit sensory information to users not only promises to radically improve the lives of amputees, but also shows potential in a range of robotic applications. Currently, a mainstream approach is to embed electrical or optical sensors with force/thermal sensing functions on the surface or inside of prosthetic fingers. Compared with electrical sensing technologies, tactile sensors based on stretchable optical waveguides have the advantages of easy fabrication, chemical safety, environmental stability, and compatibility with prosthetic structural materials.

Comprehensive chemical profiling of two Dendrobium species and identification of anti-hepatoma active constituents from Dendrobium chrysotoxum by network pharmacology.

Background: Dendrobium nobile and Dendrobium chrysotoxum are important species of the genus Dendrobium and have great economic and medicinal value. However, the medicinal properties of these two plants remain poorly understood. This study aimed to investigate the medical properties of D.

Effect of P-Glycoprotein on the Blood-Brain Barrier Transport of the Major Active Constituents of Based on the MDCK-MDR1 Cell Model.

Bunge () is a traditional Chinese medicine that has been widely used in the treatment of various central nervous system (CNS) diseases. However, the mechanism of active components of crossing the blood-brain barrier (BBB) stays unclear. The purpose of this study was to clarify the mechanism of four ingredients of , i.

Chemical profile, anti-hepatoma activity, anti-acetylcholinesterase and antioxidant activity of aerial part of Debx.

Five extracts of the aerial parts of were obtained by different solvent extraction or macroporous adsorption resin purification: ethyl acetate layer extract (EAE), n-butanol layer extract (BuE), water layer extract (WE), extract eluted by 10% ethanol from macroporous resin (10%EE), extract eluted by 80% ethanol from macroporous resin (80%EE). Antioxidant activities of the five extracts were determined by ABTS, DPPH, FRAP assays, anti-AChE activities by modified Ellman's method, anti-hepatoma activities by CCK-8 assay, and chemical constituents of 80%EE were identified by UPLC-QE-Orbitrap-MS. The results demonstrated that the 80%EE showed the best anti-hepatoma activity on Huh-7 cell line with an of 103.

In Vitro Evaluation of the Interaction of Seven Biologically Active Components in with P-gp.

The efficacy and pharmacokinetics of the biologically active components in (AR) would be affected by the interaction of P-glycoprotein(P-gp) and effective components in AR. However, little is known about the interaction between them. The goal of this research was to examine the transmembrane absorption of timosaponin AIII(TAIII), timosaponin BII(TBII), sarsasapogenin (SSG), mangiferin(MGF), neomangiferin(NMGF), isomangiferin(IMGF), and baohuosideI(BHI) in AR and their interaction with P-gp.

Construction of a novel blood brain barrier-glioma microfluidic chip model: Applications in the evaluation of permeability and anti-glioma activity of traditional Chinese medicine components.

Since most anti-glioma drug candidates hardly permeate through the blood-brain barrier (BBB), preclinical models that can integrate the complexity of the tumor microenvironment and the structure and function of the BBB is urgently needed for the treatment of glioma. Herein, we constructed an in vitro BBB-glioma microfluidic chip model lined by primary human brain microvascular endothelial cells, pericytes, astrocytes and glioma cells, which could recapitulate the high level of barrier function of the in vivo human BBB and glioma microenvironment. The BBB unit in BBB-glioma microfluidic chip (BBB-U251 chip) displayed selective permeability to fluorescein isothiocyanate isomer-dextran (FITC-dextran) with different molecular weights and three model drugs with different permeability behavior across BBB, which indicated that this glioma model included a functional barrier.

A multi-omics study of the anti-cancer effect of a ferulic acid derivative FA-30.

The active ingredients of Traditional Chinese Medicine are an important source of bioactive molecules and play an important role in the research and development of innovative drugs. FA-30, which is a derivative of natural product ferulic acid, inhibited cervical cancer cell proliferation and induced apoptosis as well. To understand the underlying mechanisms of FA-30, a complementary multi-omics study was conducted.

Screening potential P-glycoprotein inhibitors by combination of a detergent-free membrane protein extraction with surface plasmon resonance biosensor.

P-glycoprotein (P-gp) highly expressed in cancer cells can lead to multidrug resistance (MDR) and the combination of anti-cancer drugs with P-gp inhibitor has been a promising strategy to reverse MDR in cancer treatment. In this study, we established a label-free and detergent-free system combining surface plasmon resonance (SPR) biosensor with styrene maleic acid (SMA) polymer membrane proteins (MPs) stabilization technology to screen potential P-gp inhibitors. First, P-gp was extracted from MCF-7/ADR cells using SMA polymer to form SMA liposomes (SMALPs).

Investigation of the apoptosis-inducing effect of docetaxel by comprehensive LC-MS-based metabolomics and network pharmacology approaches.

Docetaxel is one of the clinical first-line drugs and its combination with other chemotherapy agents for advanced or metastatic cancers has attracted widespread attention. Therefore, to promote the clinical application of docetaxel alone or in combination, a comprehensive investigation of the metabolic mechanism of docetaxel is of great importance. Here, we apply an integrative analysis of metabolomics and network pharmacology to elucidate the underlying mechanisms of docetaxel.

Simultaneous Determination of Seven Lipophilic and Hydrophilic Components in Bunge by LC-MS/MS Method and Its Application to a Transport Study in a Blood-Brain-Barrier Cell Model.

Bunge (SM) has been extensively used in Alzheimer's disease treatment, the permeability through the blood-brain barrier (BBB) determining its efficacy. However, the transport mechanism of SM components across the BBB remains to be clarified. A simple, precise, and sensitive method using LC-MS/MS was developed for simultaneous quantification of tanshinone I (TS I), dihydrotanshinone I (DTS I), tanshinone IIA (TS IIA), cryptotanshinone (CTS), protocatechuic aldehyde (PAL), protocatechuic acid (PCTA), and caffeic acid (CFA) in transport samples.

Self-Propelled and Electrobraking Synergetic Liquid Manipulator toward Microsampling and Bioanalysis.

Droplet manipulation is of paramount significance for microfluidics-based biochips, especially for bioanalytical chips. Despite great progresses made on droplet manipulation, the existing bioanalytical methods face challenges in terms of capturing minute doses toward hard-to-obtain samples and analyzing biological samples at low temperatures immediately. To circumvent these limitations, a self-propelled and electric stimuli synergetic droplet manipulator (SES-SDM) was developed by a femtosecond laser microfabrication strategy followed by post-treatment.