GLC-mass fragmentographic determination of saccharin in biological fluids.

A specific and sensitive method is described for the determination of saccharin in biological fluids. The compound is extracted as its methyl derivative following a salt-solvent pair procedure and assayed by GLC with either flame-ionization or mass fragmentographic detection using ethylated or trideuteromethylated saccharin, respectively, as the internal standard for quantitation. Detector response was linear over concentrations of 50 mg/ml--10 micrograms/ml with multiple-ion detection mass fragmentography and from 2 micrograms/ml up to milligram levels with flame-ionization detection.

Determination of plasma and brain concentrations of trazodone and its metabolite, 1-m-chlorophenylpiperazine, by gas-liquid chromatography.

A sensitive and specific gas chromatographic procedure is described for the quantitation of trazodone and its active metabolite, 1-m-chlorophenylpiperazine (mCPP), in plasma and brain. After addition of internal standards, the samples were extracted with benzene and the extracts divided into two portions. One portion was evaporated to dryness, and residue dissolved in methanol and the solution injected into a gas chromatograph equipped with a nitrogen-selective detector, for trazodone quantitation.

Cocaine and seizure protection in mice of varying brain weights.

The effects of cocaine administration on seizure duration and proportion of animals that seized were examined in mice genetically selected for differential brain weight. In the two experiments reported, mice of the Fuller brain weight lines and of the Binghamton Heterogeneous stock were used. In Experiment 1, 21 day old male and female mice were administered either 0, 5, 10 or 20 mg/kg of cocaine hydrochloride.

Induction of mixed-function oxidase by chronic treatment with 2,3,7,8-tetrachloro-dibenzo-p-dioxin in female rats.

The effect of a 45-week treatment with different doses of 2,3,7,8-tetrachloro-dibenzo-p-dioxin (TCDD) (0.01, 0.10 and 1.

[Systolic time intervals at rest in subjects under prolonged stress: a computerized analysis].

Systolic time intervals were measured in 21 endurance-athletes (middle and lung distance runners) with a computerized method. Data from athletes were compared with that obtained in 27 age-matched normal untrained subjects. Endurance athletes showed significantly inferior values than normal untrained subjects of heart rate, total ejection time (TET), rapid ejection time (TER), electromechanical systole duration (QS2).

[Orthogonal ECG and a statistical system for computer diagnosis with ECG in chronic obstructive pneumopathy].

In 92 patients affected from chronic obstructive pulmonary disease (C.O.P.

Quantitative determination of 6-keto-PGF1 alpha released by rat aorta: comparison of mass fragmentography and high resolution gas chromatography with electron capture detection.

Mass fragmentography (MF) and high resolution gas chromatography with electron capture detection (HRGC-ECD) were used for measuring 6-keto-PGF1 alpha, the stable hydrolysis product of prostacyclin (PGI2) released by fresh rings of rat aorta, incubated in the absence of the precursors arachidonic acid or prostaglandin endoperoxide (PGH2). The incubation medium was acidified, extracted, chromatographed on silicic acid column and derivatized. Comparable results were obtained analyzing each sample by MF and HRGC-ECD.

2,3,7,8-Tetrachlorodibenzo-p-dioxin toxic effects and tissue levels in animals from the contaminated area of Seveso, Italy.

After the environmental contamination by 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) in the area of Seveso, Italy, thousands of small domestic animals (mainly rabbits and poultry) died within a few weeks. Autopsies on dead animals showed various pathological signs, such as hepatic lesions and haemorrhage. TCDD was monitored in animal tissues by gas chromatography-mass fragmentography; rabbit liver levels corresponded fairly well to soil contamination, which indicated that this species can be used as a tool for tracking the presence of TCDD in the environment.

Transient right bundle branch block unmasking anteroseptal infarction concealed by left posterior fascicular block.

A case of acute anteroseptal myocardial infarction (MI) associated with transient right bundle branch block (RBBB) and a probably persistent left posterior fascicular block is reported. When the RBBB disappeared, small initial r waves were present in the right precordial leads concealing the evidence of MI. Finally, the electrocardiographic pattern evolved to an atypical form of incomplete left bundle branch block.

Effects of gamma-butyrolactone, amphetamine, and haloperidol in mice differing in sensitivity to alcohol.

Gamma-butyrolactone (GBL) induced longer loss of righting reflex in mice (LS-line) selectively bred for greater sensitivity to ethanol than in less sensitive SS-line mice. GBL also induced a three-fold greater increase of brain dopamine levels in LS than in SS mice. Among three inbred strains, GBL-induced loss of righting reflex was greater in BALB/c, and greater in DBA/2 than in C57BL/6 mice.

Head-space gas-chromatographic analysis of vinyl chloride monomer in rat blood and tissues.

1. A method for measuring vinyl chloride monomer (VCM) concn. in rat blood and tissues is described, using a head-space g.

[Verapamil and torsade de pointe (author's transl)].

A long QT syndrome is described, followed by "torsade de pointe" that is ascribed to an hypopotassemia in a patient submitted to administration of prenylamine, vincamine, digitalis and furosemide. The efficacy of verapamil i.v.

Quantitative analysis of amineptine (S-1694) in biological samples by gas chromatography--mass fragmentography.

A sensitive, specific method for the quantitative analysis of amineptine in rat blood and brain is described. After extraction and purification amineptine is detected by mass fragmentography, monitoring the fragment ion at m/e 192. The method allows the quantitative analysis of as little as 50 ng of amineptine per ml blood and 200 ng per g for brain.

Arene oxides in styrene metabolism, a new perspective in styrene toxicity?

The metabolism of styrene was studied in the rat after intraperitoneal administration of the cold and the 14C-labeled compound. In addition to phenylethylene glycol, mandelic acid, benzoic acid and hippuric acid, phenolic metabolites, namely, 4-vinylphenol, p-hydroxymandelic acid, p-hydroxybenzoic acid, and p-hydroxyhippuric acid, were identified in the urine of the treated animals. These biotransformation products were characterized by mass spectrometry and by comparative thin layer chromatography with standard compounds.