Publications by authors named "Fan-Ping Wang"
[Effect and Mechanism of Sulforaphane on G/M Phase Arrest of Acute Myeloid Leukemia KG1a and KG1 Cells].
Zhongguo Shi Yan Xue Ye Xue Za Zhi
August 2021
Article Synopsis
- Sulforaphane (SFN) treatment for 48 hours significantly increased the number of acute myeloid leukemia cells in the G/M phase of the cell cycle, with more than half of KG1a cells and a large majority of KG1 cells showing this arrest.
- * Flow cytometry and molecular analysis indicated that SFN activates the P53 signaling pathway, leading to increased expression of P53 and P21 while decreasing CDC2, a key protein that regulates cell cycle progression.
- * The overall findings suggest that SFN could be a potential therapeutic agent by promoting G/M phase arrest in leukemia cells, thereby inhibiting their growth through the modulation of cell cycle-related gene expression.
[Effects of Arsenic Trioxide on K562 Cell Proliferation and Its Mechanisms].
Zhongguo Shi Yan Xue Ye Xue Za Zhi
December 2016
Objective: To investigate the effects of arsenic trioxide (AsO) on K562 cell proliferation by regulating cell cycle protein D1 and cyclin-dependent kinase inhibitor p27kip1.
Methods: MTT was used to detect the effect of AsO on K562 cell proliferation, so as to screen out the appropriate drug concentration. Furthermore, the K562 cell apoptosis was observed by microscopy.
[Mannan-Binding Lectin Inhibits Candida Albicans-Induced DC Maturation and Cytokine Secretion].
Zhongguo Shi Yan Xue Ye Xue Za Zhi
April 2015
Article Synopsis
- The study aimed to explore how mannan-binding lectin (MBL) affects the maturation and cytokine secretion of human dendritic cells when exposed to Candida albicans (C. albicans).
- Human dendritic cells from blood samples were cultured with varying concentrations of MBL alongside C. albicans, and various analyses were performed, including FACS and Western blot for immune markers and signaling pathways.
- Results indicated that higher concentrations of MBL reduced the expression of immune markers CD83 and CD86 and inhibited key inflammatory cytokines (TNF-α and IL-6) in response to C. albicans, suggesting MBL plays a regulatory role in the immune response to fungal infections through NF-κB signaling pathways.
[Mechanism of MBL inhibiting the LPS-induced DC maturation].
Zhongguo Shi Yan Xue Ye Xue Za Zhi
June 2013
The study was aimed to investigate the mechanism of mannan-binding lectin (MBL) on bacterial lipopolysaccharide (LPS)-induced human peripheral blood monocyte-derived dendritic cell (DC) maturation. The monocytes were prepared from the peripheral blood of healthy adult volunteers. The immature dendritic cells (imDC) were induced by 5-day-culture in medium supplemented with rhGM-CSF and rhIL-4.
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