Frequent Hand Hygiene-Induced Skin Symptoms and Alterations in Hand Microbiota: A Neglected Form of Chronic Occupational Exposure Among Health Care Workers.

Background: Frequent hand hygiene is essential for infection control among health care workers (HCWs) but may cause adverse skin effects.

Purpose: To assess the relationships between frequent hand hygiene practices, skin symptoms, and microbiota alterations in HCWs.

Methods: A comprehensive search of 7 databases was conducted to identify articles published between January 2014 and July 2024 in English and Chinese.

CREB1/CRTC2 regulated tubular epithelial-derived exosomal miR-93-3p promotes kidney injury induced by calcium oxalate via activating M1 polarization and macrophage extracellular trap formation.

Background: Calcium oxalate (CaOx) crystals are known to cause renal injury and trigger inflammatory responses. However, the role of exosome-mediated epithelial-macrophage communication in CaOx-induced kidney injury remains unclear.

Methods: To identify key molecules, miRNA sequencing was conducted on exosomes derived from CaOx-treated (CaOx-exo) and control (Ctrl-exo) epithelial cells, identifying miR-93-3p as significantly upregulated.

Prognostic and molecular multi-platform analysis of CALGB 40603 (Alliance) and public triple-negative breast cancer datasets.

Triple-negative breast cancer (TNBC) is an aggressive and heterogeneous disease that remains challenging to target with traditional therapies and to predict risk. We provide a comprehensive characterization of 238 stage II-III TNBC tumors with paired RNA and DNA sequencing data from the CALGB 40603 (Alliance) clinical trial, along with 448 stage II-III TNBC tumors with paired RNA and DNA data from three additional datasets. We identify DNA mutations associated with RNA-based subtypes, specific TP53 missense mutations compatible with potential neoantigen activity, and a consistently highly altered copy number landscape.

Identification of the Novel HLA-C*03:689 Allele by Sanger Dideoxy Nucleotide Sequencing.

The novel HLA-C*03:689 allele differs from HLA-C*03:03:01:01 by two nucleotide substitutions in codons 81 and 82 in exon 2.

GOLPH3 promotes calcium oxalate-induced renal injury and fibrosis through Golgi stress-mediated apoptosis and inflammatory responses.

A common urological disorder, calcium oxalate (CaOx) stones are the most common form of kidney stones. Deposition of CaOx crystals leads to tubular damage, interstitial fibrosis, and chronic kidney disease. Understanding the intrinsic mechanisms of kidney stone formation is essential for the prevention of kidney stones and the development of new therapeutic agents.

Arthroscopic Repair versus Conservative Treatment for first-time Anterior Shoulder Dislocation.

To systematically compare the clinical efficacy of conservative treatment and arthroscopic one-stage repair for patients with first-time anterior dislocation of the shoulder. A retrieval and systematic review of randomized controlled studies of primary anterior shoulder dislocation with conservative versus arthroscopic repair was conducted and further pooled analysis was performed to compare the overall difference in clinical outcomes, including redislocation and revision surgery rates, shoulder function scores and related complications. Preliminary meta-analysis showed that the risk of anterior shoulder redislocation was 4.

The Novel HLA-A*31:01:58 Allele, Identified by Sanger Dideoxy Nucleotide Sequencing in a Chinese Individual.

The HLA-A*31:01:58 allele differs from HLA-A*31:01:02:01 by one nucleotide substitution in codon 130 in exon 3.

A conductive polymer restores connexin43 expression through the suppression of mitogen-activated protein kinases to improve intercellular communication and alleviate atrial fibrillation.

Conductive biomaterials have shown promising results for correcting pathological cardiac electrical signaling. However, their mechanisms of operation are still largely unclear. One reason behind disrupted cardiac intercellular communication, though, is lowered expression of the gap junction protein connexin43 (Cx43), which may be alleviated by conductive biomaterials.

Multiscale Force Field Model Based on a Graph Neural Network for Complex Chemical Systems.

Inspired by the QM/MM methodology, the ML/MM approach introduces a new opportunity for multiscale simulation, improving the balance between accuracy and computational efficiency. Benefited from the rapid advancements in molecular embedding methods, density functional theory level quantum mechanical (QM) calculations within the QM/MM framework can be accelerated by several orders of magnitude through the application of machine learning (ML) potential energy surfaces. As a problem inherited from the QM/MM methodology, challenges exist in designing the interactions between machine learning and molecular mechanics (MM) regions.

Gold nanoparticles/Cu decorated metal-organic frameworks for synergistic photodynamic/ferroptosis cancer therapy.

Photodynamic therapy (PDT) holds promise for cancer treatment by generating reactive oxygen species via photosensitizers (PSs) activated by specific wavelengths of light. However, the poor water solubility of PSs and the tumor microenvironment, characterized by high glutathione (GSH) levels and hypoxia, limit its efficacy against hypoxic tumors. To overcome these challenges, we developed a novel nano-reactor, Zr(Cu)-MOF@Au@DHA, to augment PDT-ferroptosis therapy.

EZH2-mediated macrophage-to-myofibroblast transition contributes to calcium oxalate crystal-induced kidney fibrosis.

Long-term nephrocalcinosis leads to kidney injury, fibrosis, and even chronic kidney disease (CKD). Macrophage-to-myofibroblast transition (MMT) has been identified as a new mechanism in CKD, however, the effect of MMT in calcium oxalate (CaOx)-induced kidney fibrosis remains unclear. In this study, abundant MMT cells are identified by immunofluorescence (IF) and flow cytometry in kidney tissues of patients with CaOx-related CKD, a male mouse model, and CaOx-treated macrophages.

Genomic Characteristics Related to Histology-Based Immune Features in Breast Cancer.

The immune cell component of the tumor microenvironment is an important modulator of tumor progression. In patients with breast cancer, tumor-infiltrating lymphocytes (TILs) and tertiary lymphoid structures (TLS) represent core aspects of antitumor immunity, both increasingly recognized for clinical relevance. In this study, we evaluated immune-related histology features using whole-slide hematoxylin and eosin (H&E) images of The Cancer Genome Atlas Breast Invasive Carcinoma (TCGA-BRCA) data set (n = 1035) and analyzed these distinct features relative to gene expression, PAM50 subtypes, and patient survival.

Effective Nucleation Size for Ice Crystallization.

Despite the apparent simplicity of water molecules, the kinetics of ice nucleation under natural conditions can be surprisingly intricate. Previous studies have yielded critical nucleation sizes that vary widely due to differences in experimental and computational approaches. In our investigation, we employed all-atom molecular dynamics simulations to explore spontaneously grown and ideal ice nuclei, revealing significant disparities in their kinetics.

RNA-Binding Protein Lgals3, Ferroptosis, and Acute Kidney Injury.

Background: Acute kidney injury (AKI) is a syndrome characterized by a precipitous decline in kidney function, posing a significant threat to patient survival. The role of RNA binding protein (RBP) in AKI remains insufficiently understood and we found an important RBP, Lgals3, that may mediate the progress of AKI.

Methods: Lgals3 -/- mice, Nr4a1 -/- mice and cross-linking immunoprecipitation and high-throughput sequencing were performed to examine the role of Lgals3 in AKI and the targeted binding proteins.

Noninvasive assessment of a bioconductive patch for treating atrial fibrillation with magnetic resonance imaging.

Electrical and structural remodeling disrupt atrial electrical conduction, leading to atrial fibrillation (AF). Epicardially delivered conductive biomaterial patches can effectively transmit electrical signals and potentially diminish AF. However, given the progressive nature of AF development, continuous and noninvasive monitoring is essential for assessing the therapeutic efficacy of these patches over time.

Lgals3 Promotes Calcium Oxalate Crystal Formation and Kidney Injury Through Histone Lactylation-Mediated FGFR4 Activation.

The incidence of kidney stones is increasing worldwide. However, the underlying mechanism of the process of kidney stone formation and the kidney damage caused are not well understood. Here, it is observed that Lgals3, a β-galactoside-binding protein, is significantly increased in tissues with calcium oxalate (CaOx) stones, and in both in vivo and in vitro models.

UHRF1 promotes calcium oxalate-induced renal fibrosis by renal lipid deposition via bridging AMPK dephosphorylation.

Background: Nephrolithiasis, a common urinary system disorder, exhibits high morbidity and recurrence rates, correlating with renal dysfunction and the increased risk of chronic kidney disease. Nonetheless, the precise role of disrupted cellular metabolism in renal injury induced by calcium oxalate (CaOx) crystal deposition is unclear. The purpose of this study is to investigate the involvement of the ubiquitin-like protein containing PHD and RING finger structural domain 1 (UHRF1) in CaOx-induced renal fibrosis and its impacts on cellular lipid metabolism.

Circadian Clock Gene Bmal1: A Molecular Bridge from AKI to CKD.

Acute kidney injury (AKI) and chronic kidney disease (CKD) represent two frequently observed clinical conditions. AKI is characterized by an abrupt decrease in glomerular filtration rate (GFR), generally associated with elevated serum creatinine (sCr), blood urea nitrogen (BUN), and electrolyte imbalances. This condition usually persists for approximately a week, causing a transient reduction in kidney function.

Investigation of the Mechanism of Pachyman against Gout Arthritis with Network Pharmacology Analysis and Verification In Vivo.

Purpose: Pachyman, derived from Poria cocos, has been used to treat gouty arthritis (GA) for thousands of years, although its precise role and mechanisms remain unclear. Herein, we investigate the therapeutic effects of pachyman on GA and explore their underlying mechanisms.

Methods: Network pharmacology and experimental methods were employed to investigate the therapeutic mechanisms of pachyman against GA.

TRIM44 alleviates renal ischemia-reperfusion injury by inhibiting pyroptosis through the NLRP3 pathway.

Background: Renal ischemia-reperfusion injury (IRI) is a prevailing manifestation of acute kidney injury (AKI) with limited treatment options. TRIM44 has emerged as a possible target for treatment due to its regulatory function in inflammatory pathways.

Methods: In vivo and in vitro models were employed to ascertain the TRIM44 impact on renal IRI.

Noninvasive Imaging of Immune Cell Activity in Myocardial Infarction Phases Using Tc-HYNIC-mAb SPECT/CT.

Acute myocardial infarction (MI) remains a leading cause of mortality worldwide, with inflammatory and reparative phases playing critical roles in disease progression. Currently, there is a pressing need for imaging techniques to monitor immune cell infiltration and inflammation activity during these phases. We developed a novel probe, Tc-HYNIC-mAb, utilizing a monoclonal antibody that targets the voltage-gated potassium channel 1.

Unveiling ADAMTS12: A key driver of bladder cancer progression via COL3A1-Mediated activation of the FAK/PI3K/AKT signaling pathway.

Bladder cancer (BCa) is a common and lethal disease characterized by high recurrence rates and limited treatment options. Understanding the molecular pathways of BCa progress is crucial for investigating more effective targeted therapies. While ADAMTS12 is known to contribute to cancer progression and treatment resistance, its prognostic significance and underlying mechanisms in BCa remain poorly understood.

A ratiometric fluorescence sensor based on molecular imprinting technology for rapid and visual detection of norfloxacin.

The problem of excessive use and abuse of antibiotics in the environment and food biology is becoming increasingly prominent, and norfloxacin (NOR) is widely used as an inexpensive and broad-spectrum antibiotic drug. Therefore, rapid and effective detection of antibiotics and residues in biological samples has become a demand of the times. This article describes a ratiometric fluorescence sensor based on molecular imprinting technology for the rapid and visual detection of NOR.

COL10A1 Facilitates Prostate Cancer Progression by Interacting With INHBA to Activate the PI3K/AKT Pathway.

Prostate cancer (PCa) constitutes a highly common and lethal disease that impacts males globally. However, the specific molecular pathways responsible for its development are still unknown. Therefore, revealing the molecular regulators that contributed to the progression of PCa is pivotal for developing unique management strategies.