Pre-immunization of diet-induced obese male mice with inactivated pathogens increases power in a liraglutide intervention study.

Pre-immunization with inactivated antigens has been developed as an alternative to the use of 'dirty' mice, which in contrast to specific pathogen free (SPF) mice, harbour a range of pathogens. Within certain research areas, such mice are considered better models for humans than SPF mice, as they have an immune system that better mirrors human immunity. We inactivated murine adenovirus type 1 (FL), minute virus of mice, mouse hepatitis virus (A59), respirovirus muris (Sendai), Theiler's encephalomyelitis virus (GD7) and by ultraviolet irradiation.

Transparent layer constancy improves with increased naturalness of the scene.

The extent to which hue, saturation, and transmittance of thin light-transmitting layers are perceived as constant when the illumination changes (transparent layer constancy, TLC) has previously been investigated with simple stimuli in asymmetric matching tasks. In this task, a target filter is presented under one illumination and a second filter is matched under a second illumination. Although two different illuminations are applied in the stimulus generation, there is no guarantee that the stimulus will be interpreted appropriately by the visual system.

Induction of CD8 immune memory and enhanced inflammation in a skin inflammation model through pre-immunization with inactivated pathogens.

Laboratory mice live in specific pathogen-free (SPF) conditions, resulting in an immature immune system comparable to that of newborns rather than adult humans or mice from pet shops. This condition may compromise their translational value. Reintroducing pathogens would lead to the uncontrolled spread of infections and associated diseases, so research facilities should seek safer alternatives.

Meat Substitute Markets: A Comparative Analysis of Meat Analogs in Austria.

The consumption of meat substitutes has significantly grown over the last decade. To understand the extent to which plant-based meat alternatives can already substitute conventional meat in terms of price and nutritional value, detailed knowledge of current market offerings is essential. We conducted an analysis of 38 plant-based minced products and 36 plant-based sausage products in Austrian supermarkets.

Case Report of an Unusual Differential Diagnosis of a Red and Atraumatic Swollen Foot Charcot Arthropathy in Rheumatoid Arthritis.

Introduction: Neuropathic osteoarthropathy of the foot and ankle (Charcot foot) is a disease that can lead to progressive malpositioning and deformation up to complete collapse of the foot. In most cases, diabetic polyneuropathy is the underlying disease, but polyneuropathy of any cause can lead to neuropathic osteoarthropathy. Pathogenesis is still not completely understood.

Basal Proliferation and Acantholysis May Represent Histological High-Risk Factors for Progression into Invasive Squamous Cell Carcinoma: A Comparison Study in Solid Organ Transplant Recipients and Matched Immunocompetent Patients.

Histological risk factors of AKs cannot be directly determined. Recent studies indicate that AKs restricted to the lower third of the epidermis (AK I), with marked basal proliferation (PRO III) and acantholysis, are associated with an increased risk of progression to invasive squamous cell carcinoma (iSCC). To confirm the aforementioned histological risk factors, this study compared AKs from solid organ transplant recipients (sOTRs), known to carry an up to 250-fold higher risk for progression into iSCC, to a matched immunocompetent control group (ICG).

The effect of scene articulation on transparent layer constancy.

In this article, we examine the influence of scene articulation on transparent layer constancy. We argue that the term articulation may be understood as an aspect of the more general concept naturalness of a stimulus that relates to the degree of enrichment compared with a minimal stimulus and to the extent to which a stimulus contains regularities that are typically found in natural scenes. We conducted two matching experiments, in which we used strongly reduced scenes and operationalized articulation by the number of background reflectances (numerosity).

Treatment-resistant actinic keratoses are characterized by distinct clinical and histological features.

Background: Actinic keratoses (AK) are generally treated to reduce the risk of progression into invasive cutaneous squamous cell carcinoma (cSCC). However, this risk of transformation is low, and rather than focusing on these lesions, current treatment studies report on complete clearance of AKs in an entire field. This study aimed to investigate treatment-resistant AKs (trAK) after field therapy compared to randomly chosen AKs prior to treatment.

Pretreatment with ablative fractional carbon dioxide laser improves treatment efficacy in a synergistic PDT protocol for actinic keratoses on the head.

Background: A recently proposed synergistic photodynamic therapy protocol (s-PDT) combining advantages of both conventional- and daylight-PDT proved to be an effective and almost painless treatment for patients with actinic keratoses (AKs). This study investigated the safety and efficacy of an additional ablative fractional CO2-laser (AFXL) pretreatment.

Methods: 28 patients with AKs on the head received s-PDT using 5-aminolevulinic acid.

Treatment Motivations and Expectations in Patients with Actinic Keratosis: A German-Wide Multicenter, Cross-Sectional Trial.

Patient-centered motives and expectations of the treatment of actinic keratoses (AK) have received little attention until now. Hence, we aimed to profile and cluster treatment motivations and expectations among patients with AK in a nationwide multicenter, cross-sectional study including patients from 14 German skin cancer centers. Patients were asked to complete a self-administered questionnaire.

Transparent layer constancy is improved by motion, stereo disparity, highly regular background pattern, and successive presentation.

The visual system uses figural and colorimetric regularities in the retinal image to recognize optical filters and to discern the properties of the transparent overlay from properties of the background. Previous work suggests that the perceived color and transmittance of the transparent layer vary less under illumination changes than it would be expected from corresponding changes in the input. Here, we tested how the degree of this approximate transparent layer constancy (TLC) depends on factors that presumably facilitate the decomposition into a filter and a background layer.

STEAM: Using the Arts to Train Well-Rounded and Creative Scientists.

While the demand for a strong STEM workforce continues to grow, there are challenges that threaten our ability to recruit, train, and retain such a workforce in a way that is effective and sustainable and fosters innovation. One way in which we are meeting this challenge is through the use of the arts in the training of scientists. In this Perspectives article, we review the use of the arts in science education and its benefits in both K-12 and postsecondary education.

Regional lung function testing in children using electrical impedance tomography.

Objective: To evaluate regional lung function in lung-healthy children before and after exercise challenge using electrical impedance tomography (EIT).

Methods: Regional lung function was examined using EIT in 100 lung-healthy children (three age subgroups: 74-121, 122-155, 156-195 months) at baseline and 10 min after exercise. Global lung function was assessed by spirometry using Z-Scores of FEV , FVC, FEV /FVC, and FEF .

Fragility of foot process morphology in kidney podocytes arises from chaotic spatial propagation of cytoskeletal instability.

Kidney podocytes' function depends on fingerlike projections (foot processes) that interdigitate with those from neighboring cells to form the glomerular filtration barrier. The integrity of the barrier depends on spatial control of dynamics of actin cytoskeleton in the foot processes. We determined how imbalances in regulation of actin cytoskeletal dynamics could result in pathological morphology.

Multivalent Molecules as Modulators of RNA Granule Size and Composition.

RNA granules are ensembles of specific RNA and protein molecules that mediate localized translation in eukaryotic cells. The mechanisms for formation and selectivity of RNA granules are unknown. Here we present a model for assembly of one type of RNA granule based on experimentally measured binding interactions among three core multivalent molecular components necessary for such assembly: specific RNA molecules that contain a cis-acting sequence called the A2 response element (A2RE), hnRNP A2 proteins that bind specifically (with high affinity) to A2RE sequences or nonspecifically (with lower affinity) to other RNA sequences, and heptavalent protein cytoskeleton-associated protein 5 (CKAP5, an alternative name for TOG protein) that binds both hnRNP A2 molecules and RNA.

Transparent layer constancy under changes in illumination color: Does task matter?

If the perceived transmittances of transparent objects are matched across illuminants, only incomplete constancy is found. This implies that physically identical objects may appear different. Nevertheless, it was reported in the literature that subjects could almost perfectly identify such objects across illuminants, even if a transparency impression was suppressed by violating geometric transparency conditions.

Pulmonary function testing in children and infants.

Pulmonary function testing is performed in children and infants with the aim of documenting lung development with age and making diagnoses of lung diseases. In children and infants with an established lung disease, pulmonary function is tested to assess the disease progression and the efficacy of therapy. It is difficult to carry out the measurements in this age group without disturbances, so obtaining results of good quality and reproducibility is challenging.

Case management via telephone counseling and SMS for weight maintenance in adolescent obesity: study concept of the TeAM program.

Background: In-patient obesity treatment programs for adolescents are associated with good success and substantial weight loss. However, maintaining weight loss remains a challenge. This article presents the concept of the TeAM (Telephone counseling as Adiposity Management) program.

Pleomorphic ensembles: formation of large clusters composed of weakly interacting multivalent molecules.

Molecular interactions of importance to cell biology are subject to sol-gel transitions: large clusters of weakly interacting multivalent molecules (gel phase) are produced at a critical concentration of monomers. Examples include cell-cell and cell-matrix adhesions, nucleoprotein bodies, and cell signaling platforms. We use the term pleomorphic ensembles (PEs) to describe these clusters, because they have dynamic compositions and sizes and have rapid turnover of their molecular constituents; this plasticity can be highly responsive to cellular signals.

Cardiac autonomic function in adolescents operated by arterial switch surgery.

Background: Children with transposition of the great arteries, in whom an arterial switch operation (ASO) is performed, have been shown to have an increased incidence of sudden death, which may be due to cardiac autonomic imbalance and repolarisation instability. We hypothesised that i) cardiac norepinephrine (NE) kinetics and ii) arterial baroreflex sensitivity (BRS), reflecting sympathetic activity and vagal function respectively, are altered in this group.

Methods And Results: 17 children (15.

Computational analysis of Rho GTPase cycling.

The Rho family of GTPases control actin organization during diverse cellular responses (migration, cytokinesis and endocytosis). Although the primary members of this family (RhoA, Rac and Cdc42) have different downstream effects on actin remodeling, the basic mechanism involves targeting to the plasma membrane and activation by GTP binding. Our hypothesis is that the details of GTPase cycling between membrane and cytosol are key to the differential upstream regulation of these biochemical switches.

Perception of body weight status: a case control study of obese and lean children and adolescents and their parents.

Background: The objective of this study was to investigate differences in weight perception and self-concept of obese and lean children, and to examine parents' awareness of overweight in themselves and their children. A total of 59 obese patients aged 7-17 years and 49 of their parents from a pediatric obesity out-patient clinic participated and were compared with 96 normal-weight patients and 81 of their parents from a pediatric pulmonary disease out-patient clinic.

Methods: Children's and parents' self-perception of weight, desire for weight change and weight concerns, children's belief that their desired weight can be achieved, and parents' perception of their child's weight status were assessed using single questionnaire items.

A biophysical model for integration of electrical, osmotic, and pH regulation in the human bronchial epithelium.

A dynamical biophysical model for the functioning of an epithelium is presented. This model integrates the electrical and osmotic behaviors of the epithelium, taking into account intracellular conditions. The specific tissue modeled is the human bronchial epithelium, which is of particular interest, as it is the location of the most common lethal symptoms of cystic fibrosis.