Topical and intravitreous administration of cationic nanoemulsions to deliver antisense oligonucleotides directed towards VEGF KDR receptors to the eye.

Antisense oligonucleotides (ODNs) specific for VEGFR-2-(17 MER) and inhibiting HUVEC proliferation in-vitro were screened. One efficient sequence was selected and incorporated in different types of nanoemulsions the potential toxicity of which was evaluated on HUVEC and ARPE19 cells. Our results showed that below 10 microl/ml, a 2.

Quantitative assessment of bandage soft contact lens wear immediately after LASIK.

Purpose: To evaluate the efficacy and tolerability of overnight bandage soft contact lenses applied immediately after LASIK.

Methods: Bandage soft contact lenses were applied immediately following bilateral simultaneous LASIK in 161 consecutive patients. Signs and symptoms associated with contact lens wear were prospectively recorded at 1 hour, 4 hours, and the following morning, using a standardized questionnaire.

Methylprednisolone delivery to the back of the eye using hydrogel iontophoresis.

Aim: The aim of this study was to evaluate methylprednisolone penetration into ocular structures after low-current trans-scleral hydrogel iontophoresis, as compared with the common intravenous (i.v.) treatment.

In vitro and in vivo evaluation of carboplatin delivery to the eye using hydrogel-iontophoresis.

Purpose: To investigate in vitro and in vivo hydrogel-iontophoresis delivery of carboplatin to the eye.

Methods: Iontophoresis was applied on agar gels resembling the eye using different current intensities and durations. Transscleral iontophoresis was performed on healthy rabbits, applying 0, 1, and 3 mA current for 10 min.

Charged nanoparticles delivery to the eye using hydrogel iontophoresis.

Ocular iontophoresis has been investigated for many years as a non-invasive technique for enhancing ionized drug penetration through ocular tissues. In this study we assessed the penetration of charged fluorescent nanoparticles into rabbit eyes using hydrogel iontophoresis. Particle distribution into ocular tissues and penetration efficiency of negative nanoparticles compared with positive nanoparticles was also evaluated.

Methotrexate delivery to the eye using transscleral hydrogel iontophoresis.

Purpose: To evaluate methotrexate penetration and distribution profile in ocular structures after short low current transscleral hydrogel iontophoresis.

Methods: Methotrexate iontophoresis was studied in rabbits using drug-loaded hydrogels mounted on a portable iontophoretic device. Drug distribution profile was evaluated 2, 4, and 8 hours after iontophoretic treatment of 1.

Early transient visual acuity loss after LASIK due to steroid-induced elevation of intraocular pressure.

Purpose: To report the clinical course of early transient reduction of uncorrected visual acuity (UCVA) after LASIK surgery resulting from steroid-induced elevation of intraocular pressure (IOP).

Methods: Twenty-nine eyes of 15 patients who received topical corticosteroids after uneventful myopic LASIK surgery and had a decrease in UCVA within the first 3 weeks were evaluated retrospectively.

Results: Intraocular pressure increased by 4 to 30 mmHg from preoperative to postoperative days 4 to 20.

Conjunctival autografting combined with low-dose mitomycin C for prevention of primary pterygium recurrence.

Purpose: To compare the clinical outcome of pterygium surgery combining intraoperative mitomycin C (MMC) with a free conjunctival autograft, with three other methods of pterygium surgery, including intraoperative MMC alone, conjunctival autograft alone, and bare sclera without adjunctive treatment.

Design: Interventional, randomized and in part nonrandomized, prospective, comparative study.

Methods: setting: A university medical center department of ophthalmology.