Evaluating canalside hedgerows to determine future interventions.

This paper describes a project undertaken during 2001/2002 which developed a method for valuing hedgerows adjacent to the inland waterway network of Great Britain. The method enables the landowner, British Waterways, to manage their valuable environmental asset to achieve a good level of biodiversity and robust habitat balanced against the heavy amenity use the 3000 km canal network endures. Valuation techniques were developed using a combination of new and existing ecological indices for components of biodiversity, hedgerow structure and amenity, and synthesised into an index in an innovative combined approach.

Microheterogeneity and sialic acid in human plasma angiotensinogens in various physiological states.

Pooled or individual plasmas from normal men, women, pregnant women (third trimester), anephric women, and rat liver perfusates were used as sources of angiotensinogen. The plasmas were fractionated and desalted by Sephadex gel filtration, then subjected to isoelectric focusing in a pH 4 to 6 gradient on 40 X 4 cm slabs of polyacrylamide gel. The gels were cut transversely into 0.

Resolution of rat renin substrates by isoelectric focusing.

Pooled plasmas from normal or binephrectomized rats and perfusates of isolated livers were used as sources of renin substrate for isoelectric focusing. After desalting, preliminary fractionation (plasma only), and concentration, the preparations were focused in a pH 3--10 gradient on 20-cm glass plates layered with Sephadex slurry. The pH 4--6 region, containing all the substrate, was scraped from this plate and refocused in a pH 4--6 gradient.

Inhibition of the reconstitution of the haemolytic activity of the first component of human complement by a pepsin-derived fragment of subcomponent C1q.

1. A fragment of subcomponent C1q, which contained all the collagen-like features present in the intact molecule, was isolated by pepsin digestion as described by Reid [Biochem. J.

Pathway mediating hypotension elicited by stimulation of the amygdala in the rat.

Cardiovascular responses were elicited from 372 histologically verified sites in the amygdala and closely adjacent structures in 82 rats under urethan or chloralose anesthesia. Arterial hypotension was elicited at low current intensities from the medial, central, lateral, and basal nuclei of rats under either anesthetic; the hypotension was still elicited in artificially respired paralyzed rats. Stimulation of the cortical nucleus produced variable changes in arterial pressure.

Central neural regulation of heart and blood vessels in mammals.

The study of the central regulation of the circulation in the past has been directed primarily at observing reflex responses to stimulation of peripheral receptors and at producing changes in cardiovascular parameters during electrical stimulation of central sites. These studies have demonstrated that the nervous system can regulate the circulation to different vascular beds with a high degree of specificity and that it has the ability to provide a range of coordinated responses which are appropriate to the metabolic needs of a particular behavioural pattern. In addition, it has become firmly established that the nervous system is capable of coupling cardiovascular changes with other autonomic and somatic activities to produce an integrated response.