Sensitive and selective real-time electrochemical monitoring of DNA repair.

Unrepaired DNA damage can lead to mutation, cancer, and death of cells or organisms. However, due to the subtlety of DNA damage, it is difficult to sense the presence of damage repair with high selectivity and sensitivity. We have shown sensitive and selective electrochemical sensing of 8-oxoguanine and uracil repair glycosylase activity within DNA monolayers on gold by multiplexed analysis with silicon chips and low-cost electrospun nanofibers.