Dedifferentiated fat cells-derived exosomes (DFATs-Exos) loaded in GelMA accelerated diabetic wound healing through Wnt/β-catenin pathway.

Background: Diabetic foot ulcers pose significant challenges for clinicians worldwide. Cell-free exosome therapy holds great potential for wound healing. Dedifferentiated fat cells (DFATs) have been used in tissue engineering and regeneration, but there are no reports on the use of DFATs-derived exosomes in diabetic wound repair.

Effects of omega-3 polyunsaturated fatty acids on inflammation resolution and angiogenesis in fat grafts in a controlled mouse model.

Background: The upregulation or delay of acute inflammation at any stage limits fat graft survival. Active endogenous inflammation resolution mechanisms and mediators are novel therapeutic tools for inflammation. This study explored the effects of supplementation of omega-3 polyunsaturated fatty acids (PUFAs) deriving specialized proresolving mediators (SPMs) on postoperative inflammation and graft survival in vivo.

Innovative Mechanical Processing of Adipose Tissue: A Comparative Evaluation of Microfat and Concentrated Micronized Fat for Superficial Fat Grafting.

Background: Superficial autologous fat grafting is widely used for facial rejuvenation and regenerative treatments, yet its retention rate remains unpredictable. Enhancing the concentration of adipose-derived stem cells (ASCs) has been shown to improve graft retention. Mechanical fat processing techniques can reduce fat granules size for superficial injection as well as elevate the concentration of ASCs.

Microliposuction and Radiofrequency Combined With Fat Grating as a New Method for Hybrid-Type Nasolabial Folds.

Backgrounds: The hybrid-type nasolabial fold is a special, complex type of nasolabial fold with various causes, making it difficult to obtain an ideal effect with a single treatment.

Objectives: This study aimed to investigate the effect and complications of a comprehensive therapy combining microliposuction, radiofrequency, and fat grafting on hybrid-type nasolabial folds.

Results: Twenty-one patients aged 27 to 45 (mean: 34.

Evaluating the Efficacy of Dedifferentiated Fat Cells (DFATs) vs Adipose-Derived Stem Cells (ASCs) in Enhancing the Viability of Fat Grafts.

Background: Enhancing graft fat survival remains a paramount challenge in autologous fat transplantation surgeries. Dedifferentiated fat cells (DFATs) and adipose-derived stem cells (ASCs) represent 2 pivotal cells with potential to improve fat graft survival rates.

Objectives: In this study we aimed to compare the effectiveness of DFATs and ASCs in promoting fat graft survival, emphasizing their adipogenic and angiogenic capabilities.

The impact of SARS-Cov-2 infection on the periocular injection pain and hypersensitive reaction to botulinum toxin type A: results from clinical questionnaires.

Background: The COVID-19 pandemic has brought about significant changes in the medical field, yet the use of botulinum toxin type A has remained uninterrupted. Plastic surgeons must carefully consider the timing of administering botulinum toxin type A to patients who have recovered from COVID-19.

Methods: A questionnaire survey was conducted among patients who had contracted and recovered from SARS-CoV-2 within a month.

Deep-learning based segmentation of ultrasound adipose image for liposuction.

Background: To develop an automatic and reliable ultrasonic visual system for robot- or computer-assisted liposuction, we examined the use of deep learning for the segmentation of adipose ultrasound images in clinical and educational settings.

Methods: To segment adipose layers, it is proposed to use an Attention Skip-Convolutions ResU-Net (Attention SCResU-Net) consisting of SC residual blocks, attention gates and U-Net architecture. Transfer learning is utilised to compensate for the deficiency of clinical data.

Buccal Fat Pad Augmentation for Midfacial Rejuvenation: Modified Fat Grafting Technique and Ogee Line Remodeling.

Background: The volume and position of the buccal fat pad (BFP) change with age, which manifests as a hollow midface. Previous studies showed that autologous fat grafting for BFP augmentation could effectively ameliorate midfacial hollowing.

Objectives: The aim of this study was to introduce a modified fat grafting technique for female patients with midfacial hollowing to restore the volume of BFP, and to evaluate the safety and effectiveness of this approach.

Comparison of Microfat, Nanofat, and Extracellular Matrix/Stromal Vascular Fraction Gel for Skin Rejuvenation: Basic Animal Research.

Background: Although stromal vascular fraction (SVF) cells and adipose-derived stem cells have well-defined antiaging effects on skin, certain disadvantages have limited their clinical application.

Objectives: The aim of this study was to evaluate the effects of microfat, nanofat, and SVF-gel in improving ultraviolet (UV)-induced photoaged skin injury in nude mice.

Methods: After successfully establishing a photoaging model by UVA and UVB irradiation in nude mice, the back of each mouse was divided into 2 regions and randomly injected under the dermis with 0.

Pyramidal multiple-theory (multi-type, multi-method and multi-layer) for facial fat grafting.

Background: Age-related changes to the face pertinent anatomy and important aspects of autologous fat grafting have been widely applied in facial rejuvenation. Various types of autologous fat products (Macrofat, Microfat, SEFF, nanofat, and SVF-gel) with different properties and applicabilities have been introduced and available for surgeons.

Methods: Key differences between common techniques for fat processing and infiltration.

[Autologous fat grafting for perioral and lower face rejuvenation].

Objective: To review the research progress of the application of autologous fat grafting in perioral and lower face rejuvenation.

Methods: By extensively reviewing related articles and combining the clinical experiences, the anatomic features (fat compartments and blood supply), aging features of the perioral and lower face, and the application advancement and related complications of autologous fat grafting in this area were generally summarized.

Results: The aesthetic features of perioral and lower face include the lip, chin, and so on, and the anatomic structures relating autologous fat grafting include the mandibular fat compartments, chin fat compartments, and other fat compartments.

Increased Fat Graft Survival by Promoting Adipocyte Dedifferentiation.

Background: Some adipocytes undergo dedifferentiation after fat transplantation, and this may affect the survival of fat grafts. However, this effect has not been adequately studied.

Objectives: This study aimed to clarify the effect of promoting the dedifferentiation of mature adipocytes on the survival of fat grafts.

Facial Rejuvenation and Contouring with Radiofrequency-Assisted Procedures in Asians.

Radiofrequency (RF)-assisted liposuction treatment is a minimally invasive skin-tightening technique for the patient population with skin laxity. The authors recommend facial liposuction combined with the RF procedure for the treatment of skin laxity. Minimal-invasive liposuction creates working channels for RF treatment and sufficiently exposes the subdermal fibrous septal network tissue so that the RF energy can directly act on the collagen of the fibrous septal network for thermal shrinkage, leading to better surgical results.

An explainable machine learning method for assessing surgical skill in liposuction surgery.

Purpose: Surgical skill assessment has received growing interest in surgery training and quality control due to its essential role in competency assessment and trainee feedback. However, the current assessment methods rarely provide corresponding feedback guidance while giving ability evaluation. We aim to validate an explainable surgical skill assessment method that automatically evaluates the trainee performance of liposuction surgery and provides visual postoperative and real-time feedback.

Microliposuction and radiofrequency combined with the thread technique as a new method used in lower face lifting.

Background: Minimally invasive facial rejuvenation is a popular and well-established treatment for patients seeking facial lifting.

Objective: The authors combined facial microliposuction, radiofrequency, and thread lifting technology as a comprehensive treatment (LRT) for facial rejuvenation in a case series of 57 patients.

Methods: A total of 76 patients underwent surgery and 75% completed 6 months of follow-up (57 patients); the patients were aged 26 to 51 (33.

Function-preserving fat grafting in the breast: Results based on 18 years of experience.

Background: Autologous fat transplantation has been used for breast nearly 40 years, but there are two main problems: the volume retention rate is unpredictable, leading to too many operations, and various complications, such as nodules, necrosis and calcification, occur.

Objective: The author proposed "function-preserving fat grafting" (FPFG) and reviewed the clinical data of patients from October 2002 to December 2020.

Methods: A total of 1218 patients underwent surgery, and 767 patients were followed up for more than half a year.

Types of periocular wrinkles based on anatomical and contractile characteristics of participating periocular muscles: A modified classification method and personalized injection technique.

Background: Clinical classification methods for periocular wrinkles based on participating muscle behavior are lacking, and traditional treatment lacks detailed and individualized methods.

Aims: This study aimed to propose a modified classification system and individualized treatment for periocular wrinkles based on the participating muscles.

Patients/methods: Periocular wrinkles were classified into types I.

The Importance of Protecting the Structure and Viability of Adipose Tissue for Fat Grafting.

Background: Fat grafting is widely used for soft-tissue augmentation; however, the related clinical outcome remains variable and technique-dependent. The mechanisms underlying fat graft survival are not fully understood, particularly regarding the contributions of different cell types, such as functional adipocytes. This study evaluated the importance of adipose tissue structure and viability in fat grafting and, to some extent, revealed the effect of adipocytes in fat grafting.

[Research progress of external volume expansion assisted autologous fat grafting for breast reconstruction].

Objective: To review the application progress, mechanism, application points, limitations, and oncological safety of external volume expansion (EVE) assisted autologous fat grafting for breast reconstruction and provide a reference for optimizing the design of EVE.

Methods: Based on the latest relevant articles, the basic experiments and clinical applications of EVE were summarized.

Results: EVE can reduce interstitial fluid pressure, increase blood supply, and promote adipogenic differentiation, thereby benefiting the survival of transplanted fat.

Washing Lipoaspirate Improves Fat Graft Survival in Nude Mice.

Background: The optimal fat processing technique of fat grafting has not been determined. We have proved the importance of washing lipoaspirate to remove blood, but the necessity of washing when there is no obvious bleeding during liposuction is not clear.

Objectives: The purpose of this study is to further investigate the effect of washing on fat graft survival and the underlying mechanisms, from the perspective of inflammation, oxidative stress and apoptosis.

Single session of combined microfat and subcision in the treatment of Grade 4 atrophic acne scars.

Background: Severe atrophic acne scars (AAS) remain the "Achilles's heel" of AAS treatment. The combination of microfat and subcision treatment is a potential solution for severe AAS.

Aims: This study aimed to evaluate the efficacy and safety of combined microfat and subcision treatment for severe (Grade 4) AAS.