Identification of additives in polymers from single-use bioprocessing bags by accelerated solvent extraction and ultra-high performance liquid chromatography coupled with high-resolution mass spectrometry.

Single-use technologies are increasingly used in biopharmaceutical manufacturing. Despite their advantages, these plastic assemblies draw concern because they are a potential source of contamination due to extractable and leachable compounds (E&Ls). Characterising E&Ls from such materials is a necessary step in establishing their suitability for use.

Accelerated solvent extraction by using an 'in-line' clean-up approach for multiresidue analysis of pesticides in organic honey.

The worldwide loss of honeybee colonies may be due to their exposure to several contaminants (i.e., pesticides); such contamination may also have impacts on consumers' health.

Regioselective carbene insertion on polysubstituted dihydroaromatic compounds.

[reaction: see text] The rhodium-catalyzed CH-insertion reaction of diazo ester derivatives into dihydroaromatic compounds was expanded to the application of various polysubstituted and functionalized 1,4-dienes generated from a cobalt-catalyzed Diels-Alder reaction. The highly regioselective CH-insertion process produces after DDQ oxidation polysubstituted, polyfunctionalized aromatic compounds in good overall yields.

Highly diastereoselective cyanation of methyl ketimines obtained from (R)-glyceraldehyde.

[reaction: see text] Addition of trimethylsilyl cyanide to ketimines derived from (R)-2,2-dimethyl-1,3-dioxolan-4-yl methyl ketone to generate a quaternary stereocenter has been achieved with high yields and excellent diastereoselectivity. The stereoselectivity was found to be temperature and solvent dependent. The beta-hydroxy-alpha-amino nitrile of syn configuration was the major compound in kinetically controlled reactions, whereas the anti stereoisomer was obtained in excess in thermodynamically controlled reactions.

A simple cobalt catalyst system for the efficient and regioselective cyclotrimerisation of alkynes.

The intermolecular cyclotrimerisation of terminal and internal alkynes can be catalysed by simple cobalt complexes such as a CoBr2(diimine) under mild reaction conditions when treated with zinc and zinc iodide with high regioselectivity in excellent yields.