In vitro assessment of NaF/Chit supramolecular complex: Colloidal stability, antibacterial activity and enamel protection against S. mutans biofilm.

Objectives: This study assessed the effect of NaF/Chit suspensions on enamel and on S. mutans biofilm, simulating application of a mouthrinse.

Methods: The NaF/Chit particle suspensions were prepared at molar ratio [NaF]/Chit]≈0.

Building an explanatory model for snakebite envenoming care in the Brazilian Amazon from the indigenous caregivers' perspective.

Background: In the Brazilian Amazon, snakebite envenomings (SBE) disproportionately affect indigenous peoples. Communication between indigenous and biomedical health sectors in regards to SBEs has never been explored in this region. This study aims to build an explanatory model (EM) of the indigenous healthcare domain for SBE patients from the perspective of the indigenous caregivers.

Which self-etch acidic composition may result in higher dental bonds at the long-term? A network meta-analysis review of in vitro studies.

Objectives: This review evaluated the effects of the acidic composition of self-etch (SE) adhesives at the long-term bond strengths to dentin and enamel.

Data: The review followed the PRISMA Extension Statement for network meta-analysis. Studies were identified by a systematic search in PubMed, Web of Science, and Scopus databases.

Possible Relationship Between the Oral and Gut Microbiome, Caries Development, and Obesity in Children During the COVID-19 Pandemic.

The COVID-19 pandemic has brought health damage and socioeconomic disruptions, together with lifestyle disorders around the world. Children are one of the most commonly affected, mainly due to social isolation and changes in eating habits and physical activities. This way, the risk of weight gain and obesity is possibly enhanced, as well as poor oral hygiene conditions and early childhood caries (ECC) development during the lockdown.

Synthesis and characterization of TPP/chitosan nanoparticles: Colloidal mechanism of reaction and antifungal effect on C. albicans biofilm formation.

In the present study chitosan (Chit) nanoparticles were synthetized by the ionic gelation process, using tripolyphosphate (TPP) as crosslinking agent. The TPP/Chit nanoparticle formation was evaluated by titrations, measuring electrical conductivity (k), zeta potential (ZP), hydrodynamic diameter (D), viscosity (η) and heat by isothermal calorimetry (ITC). The antifungal effects were evaluated by C.

Effect of chitosan nanoparticles on the inhibition of Candida spp. biofilm on denture base surface.

Objectives: Chitosan nanoparticles (ChNPs) have antifungal effects, however there is a lack of information about the effects of ChNPs against Candida biofilm on denture base surface. This study investigated the ChNPs effect against C. albicans biofilm adhesion and formation, and against Candida spp.

Antifungal activity, mode of action and anti-biofilm effects of Laurus nobilis Linnaeus essential oil against Candida spp.

Objective: The present study demonstrated the antifungal potential of the chemically characterized essential oil (EO) of Laurus nobilis L. (bay laurel) against Candida spp. biofilm adhesion and formation, and further established its mode of action on C.

In vitro effect of S. mutans biofilm on fluoride/MDPB-containing adhesive system bonded to caries-affected primary dentin.

Purpose: To evaluate the effect of storage in S. mutans culture or water on the durability of fluoride and MDPB-containing adhesive system on artificial caries-affected primary dentin.

Methods: Dentin flat surfaces of primary molars were submitted to artificial caries development using S.

In vitro effects of nano-hydroxyapatite paste on initial enamel carious lesions.

Purpose: The purpose of this study was to analyze the protective effect of remineralizing agents on enamel caries lesions using surface Knoop microhardness testing (KHN) and atomic force microscopy (AFM).

Methods: Forty-eight human enamel blocks were assigned to four groups (N=12): (1) control (without agent); (2) fluoride varnish (Duraphat); (3) nano-HAP paste (Desensibilize Nano P); and (4) casein phosphopeptide-amorphous calcium phosphate (CPP-ACP) paste (MI Paste Plus). Incipient caries-like lesions were artificially developed.

In Vitro Effects of Nano-hydroxyapatite Paste on Initial Enamel Carious Lesions.

Purpose: The purpose of this study was to analyze the protective effect of remineralizing agents on enamel caries lesions using surface Knoop microhardness testing (KHN) and atomic force microscopy (AFM).

Methods: Forty-eight human enamel blocks were assigned to four groups (N=12): (1) control (without agent); (2) fluoride varnish (Duraphat); (3) nano-HAP paste (Desensibilize Nano P); and (4) casein phosphopeptide-amorphous calcium phosphate (CPP-ACP) paste (MI Paste Plus). Incipient caries-like lesions were artificially developed.

In vivo biocompatibility versus degree of conversion of resin-reinforced cements in different time periods.

This study focused on test the null hypothesis that there is no difference between the degree of conversion and biocompatibility of different resin reinforced glass ionomer cements (RRGICs). Forty-eight male Wistar rats were used, distributed into four groups (n = 12), as follows: Group C (Control, polyethylene), Group FOB (Fuji Ortho Band), Group UBL (Ultra band Lok), and Group MCG (Multicure Glass), in subcutaneous tissue. The events of edema, necrosis, granulation tissue, multinuclear giant cells, young fibroblasts, and collagen formation were analyzed at 7, 15, and 30 days.

In vitro effect of calcium nanophosphate and high-concentrated fluoride agents on enamel erosion: an AFM study.

Background: Calcium nanophosphate paste can provide ions to remineralize enamel. There are, however, no data available about the remineralizing effect of this paste on the prevention of enamel erosion, when compared with highly concentrated fluoride agents.

Aim: To analyze the effect of calcium nanophosphate paste, fluoride gel, and varnish to protect against enamel erosion using surface Knoop hardness (KNH) and atomic force microscopy (AFM).

Influence of degree of conversion on the biocompatibility of different composites in vivo.

Purpose: To evaluate the relationship between biocompatibility and degree of monomer conversion of composites used to bond brackets to enamel, porcelain, resin, or metal surfaces at different time intervals.

Materials And Methods: Twenty-four male Wistar rats were used, divided into 4 groups (n = 6) as follows: group C (control, polyethylene), group TCC (Transbond Color Change), group QC (Quick-Cure), and group EB (Eagle Bond). These substances were inserted into subcutaneous tissue.

Biomechanical degradation of the nano-filled resin-modified glass-ionomer surface.

Purpose: To evaluate in the laboratory the roughness (Ra) and micromorphology surface of the nanofilled resin-modified glass-ionomer (Ketac N100) subjected to biomechanical degradation, compared to Vitremer, Ketac Molar Easymix and Fuji IX.

Methods: Specimens obtained from the ionomers were divided into two storage groups (n = 10): relative humidity and S. mutans biofilm (biodegradation).

Assessment of groundwater quality in a region of endemic fluorosis in the northeast of Brazil.

The aim of this study was to estimate the risk for caries and fluorosis in a desertification area, applying the calcium/fluoride concentration ratio of underground water and the quality of water in a selected geographical region. This study was performed in the municipality of São João do Rio do Peixe, located in the tropical semiarid lands of Brazil. A total of 111 groundwater samples were collected.

Influence of NaOCl irrigation and water storage on the degradation and microstructure of the resin/primary dentin interface.

Purpose: To evaluate the influence of NaOCl irrigation and water storage on the degradation and microstructure of the resin/dentin interface of primary teeth bonded with three different adhesive systems using the microtensile bond strength test (μTBS) and scanning electron microscopy (SEM).

Materials And Methods: Ninety sound primary molars were used. Eighteen groups were formed according to different adhesive systems (Adper Single Bond 2, SB; Clearfil Protect Bond, CP; Adper Prompt L-Pop, APL) with or without 0.

Analyses of biofilms accumulated on dental restorative materials.

Purpose: To qualitatively and quantitatively assess the architectural arrangement of microorganisms in biofilm developed on the surface of different restorative materials: ceramic (C), resin composite (RC), conventional (CGIC) and resin-modified glass-ionomer cements (RMGIC).

Methods: Streptococcus mutans was used to develop a biofilm that adhered to the surfaces of the selected material disks in 30 days. The specimens were stained and analyzed by confocal laser scanning microscopy and COMSTAT.

Mineral distribution and CLSM analysis of secondary caries inhibition by fluoride/MDPB-containing adhesive system after cariogenic challenges.

Objective: To evaluate the inhibition zone formation (IZ) and mineral distribution along the interface of adhesive systems either containing fluoride and antibacterial primer or not, after chemical and biological artificial caries challenges.

Methods: Forty-eight third molars were used. Artificial caries was developed with S.

Confocal laser scanning microscopic analysis of the depth of dentin caries-like lesions in primary and permanent teeth.

This study analyzed comparatively, by confocal laser scanning microscopy (CLSM), the depth of caries-like lesions produced by biological and chemical artificial models in permanent and primary dentin. Six primary molars and six premolars were used. The occlusal enamel was removed and a nail polish layer was applied on the specimens, except for a 4 x 2 mm area on dentin surface.

Presence of mutans streptococci and Candida spp. in dental plaque/dentine of carious teeth and early childhood caries.

This study determined the presence of mutans streptococci and Candida spp. in supragingival dental plaque and infected dentine of caries-free children, with early childhood caries and caries. Pooled samples of dental plaque and infected dentine were collected from 56 children aged 1-5 years, which were divided into 3 groups: early childhood caries (ECC); caries and caries-free.