Cytotoxicity of Chelating Agents Used In Endodontics and Their Influence on MMPs of Cell Membranes.

This study evaluated the cytotoxic effect and the ability to inhibit matrix metalloproteinases (MMP-2 and MMP-9) of 0.2% chitosan (CH) and 1% acetic acid (AA) compared with 17% ethylenediaminetetraacetic acid (EDTA). Cell viability assay was performed according to ISO 10993-5 with mouse fibroblasts (L929).

Influence of Cervical Preflaring on the Incidence of Root Dentin Defects.

Introduction: This study evaluated the influence of cervical preflaring on the incidence of root dentin defects after root canal preparation.

Methods: Extracted human maxillary central incisors were selected and allocated to 1 control group and 12 experimental groups (n = 15). Teeth in the control group were left unprepared, whereas the others were prepared using 2 reciprocating single-file systems (Reciproc and WaveOne [WO]), 3 full-sequence rotary systems (ProTaper Universal, ProTaper Next [PTN], and ProFile), and K-files driven by an oscillatory system, with and without cervical preflaring.

Forensic DNA typing from teeth using demineralized root tips.

Teeth are widely used samples in forensic human genetic identification due to their persistence and practical sampling and processing. Their processing, however, has changed very little in the last 20 years, usually including powdering or pulverization of the tooth. The objective of this study was to present demineralized root tips as DNA sources while, at the same time, not involving powdering the samples or expensive equipment for teeth processing.

Assessment of the Amount of Calcium Ions Released after the use of Different Chelating Agents and Agitation Protocols.

Background: The main goal of endodontic treatment is to achieve cleaning and shaping prior to the filling process.

Objective: This study aimed to evaluate, using atomic absorption spectrometry, the release of Calcium ions after the use of different chelating agents and protocols of agitation.

Method: Ninety human canine teeth were randomly assigned to one of nine groups (n=10), as follows: 1) 0.

Root Canal Transportation and Centering Ability of Nickel-Titanium Rotary Instruments in Mandibular Premolars Assessed Using Cone-Beam Computed Tomography.

Introduction: The aim of this study was to evaluate, using cone-beam computed tomography (CBCT), transportation and centralization of different nickel-titanium (NiTi) rotary instruments.

Methods: One hundred and twenty eight mandibular premolars were selected and instrumented using the following brands of NiTi files: WaveOne, WaveOne Gold, Reciproc, ProTaper Next, ProTaper Gold, Mtwo, BioRaCe and RaCe. CBCT imaging was performed before and after root canal preparation to obtain measurements of mesial and distal dentin walls and calculations of root canal transportation and centralization.

Cariogenic microbiota of children under chemotherapy: A pilot study.

Aim: To analyze the cariogenic microbiota of children who are under antineoplastic therapy.

Methods: Twenty-seven patients undergoing cancer treatment in the Pediatric Unit of Mato Grosso Cancer Hospital were selected. The inclusion criteria of volunteers were being under chemotherapy in the saliva collection period, age between 2 and 18 years, collaborate with the protocol for clinical examination and saliva collection, and responsible have given their written consent.

The Influence of Cervical Preflaring on the Amount of Apically Extruded Debris after Root Canal Preparation Using Different Instrumentation Systems.

Introduction: The objective of this study was to evaluate the influence of cervical preflaring on the amount of apically extruded debris after root canal preparation using different instrumentation systems.

Methods: One hundred eighty human maxillary central incisors were selected and randomly divided into 12 groups (n = 15). Root canals were instrumented according to manufacturers' instructions using 2 reciprocating single-file systems (Reciproc [VDW, Munich, Germany] and WaveOne [Dentsply Maillefer, Ballaigues, Switzerland]), 3 full-sequence rotary systems (ProTaper Universal [Dentsply Maillefer], ProTaper Next [Dentsply Tulsa Dental, Tulsa, OK], and ProFile [Dentsply Maillefer]), and K-files (Dentsply Maillefer) driven by an oscillatory system with and without cervical preflaring.

Effect of repeated sterilization cycles on the physical properties of scaling instruments: a scanning electron microscopy study.

Background: Repeated sterilizations cycles cause undesirable alterations in the material properties of the instruments, such as corrosion, alterations in the hardness of the metal and the loss of the cutting sharpness of the instrument. This research examined the effect of repeated dry heat sterilization and autoclaves cycles on carbon steel (CS) and stainless steel (SS) curettes during the scaling and root planning.

Materials And Methods: A total of 77 Gracey curettes were used in this study.

Analysis of metal contents in Portland Type V and MTA-based cements.

The aim of this study was to determine, by Atomic Absorption Spectrometry (AAS), the concentration levels of 11 metals in Type V gray and structural white PC, ProRoot MTA, and MTA Bio. Samples, containing one gram of each tested cement, were prepared and transferred to a 100 mL Teflon tube with a mixture of 7.0 mL of nitric acid and 21 mL of hydrochloric acid.

Tecidual reaction of calcium hydroxide front chronic stress histological study in rats.

Background: The Calcium Hydroxide has been widely used as an intracanal dressing and in combination with restorative and endodontic materials and its main goal is the tissue reparation. However, when the patient has chronic stress, the immunological response and tissue repair decreases in both the epithelial and connective tissue. Therefore, the aim was to analyze the effect of chronic stress on the tissue response in rats exposed to calcium hydroxide (CH).

Comparative analysis of physicochemical properties of root perforation sealer materials.

Objectives: This study evaluated the solubility, dimensional alteration, pH, electrical conductivity, and radiopacity of root perforation sealer materials.

Materials And Methods: For the pH test, the samples were immersed in distilled water for different periods of time. Then, the samples were retained in plastic recipients, and the electrical conductivity of the solution was measured.

Prevalence of impacted teeth in a Brazilian subpopulation.

The objective of this study was to estimate the prevalence of impacted teeth in a subpopulation of Brazilian patients based on the retrospective analysis of panoramic radiographs obtained at an oral radiology clinic. Out of 1,977 panoramic radiographs, 1,352 fulfilled inclusion criteria, and 22,984 teeth were assessed. Data were statistically analyzed using Kolmogorov-Smirnov's and Levene's tests; significance was set at 5%.

Customized polymethyl methacrylate implants for the reconstruction of craniofacial osseous defects.

Craniofacial defects represent alterations in the anatomy and morphology of the cranial vault and the facial bones that potentially affect an individual's psychological and social well-being. Although a variety of techniques and restorative procedures have been described for the reconstruction of the affected area, polymethyl methacrylate (PMMA), a biocompatible and nondegradable acrylic resin-based implant, is the most widely used alloplastic material for such craniomaxillofacial reconstruction. The aim of this study was to describe a technique for aesthetic and functional preoperative customized reconstruction of craniofacial bone defects from a small series of patients offered by the Brazilian public health system.

Using cone beam computed tomography images to diagnose multiple taurodontisms.

Taurodontism is a developmental anomaly that manifests as an alteration in a patient's internal morphology and is characterized by the lack of constriction at the level of the cementoenamel junction, which results in an apical extension of the pulp chamber that extends into the root area of a multiradicular tooth. This report presents a case of an otherwise healthy patient with 12 hypertaurodontic teeth, which were detected by using cone beam computed tomography (CBCT). It is essential to emphasize the importance of routine imaging examinations for diagnosing developmental dental anomalies such as taurodontism, as this condition is generally not found by routine oral examination, and is not necessarily restricted to syndromic patients.

Growth hormone insensitivity syndrome: unusual oral manifestations.

Children with significant growth retardation and normal levels of growth hormone are diagnosed with growth hormone insensitivity. The main oral findings observed in patients with growth hormone insensitivity syndrome (GHIS) are underdeveloped jaws, crowded teeth and delayed eruption of permanent teeth. This manuscript describes a 9-year-old child diagnosed with GHIS, who had delayed eruption of permanent teeth and 14 unerupted supernumerary teeth.

When and Why Parents Seek Dental Care for Children under 36 Months.

Background: The aim was to analyze an infant preventive program determining at what age parents take their children for their first dental visit and the reasons why they do it.

Materials & Methods: A total of 844 children aged from 0 to 36 months, enrolled in the program of oral health maintenance of the Baby Clinic, participated in this study. During the first dental visit, the parents were inquired about the reasons that led them to enroll their children in the program.

Lipid Profile Parameters under influence of Periodontitis Associated with Chronic Stress: An Animal Model Study.

Background: The present study compared the effect of two chronic stress models associated with periodontitis induced in rats on partial lipid profile parameters.

Materials & Methods: Forty-eight rats were divided into 4 groups: physical stress+periodontitis, variable stress+periodontitis, periodontitis and control. Physical stress and variable stress occurred over 60 days.

Degree of conversion of polymer-matrix composite assessed by FTIR analysis.

Aims And Objectives: The behavior of polymer-matrix composite is dependent on the degree of conversion. The aim of this study was to evaluate the degree of conversion of two resin cements following storage at 37°C immediately, 24 and 48 hours, and 7 days after light-curing by FTIR analysis.

Materials And Methods: The specimens were made in a metallic mold and cured with blue LED with power density of 500 mW/ cm(2) for 30 seconds.

Conventional Treatment of Maxillary Incisor Type III Dens Invaginatus with Periapical Lesion: A Case Report.

Dens invaginatus is a developmental dental anomaly clinically characterized by a palatine furrow that can be limited to the coronal pulp or may extend to the radicular apex. The purpose of this paper was to present a clinical case of type III dens invaginatus, identified on the maxillary right central incisor in anterior periapical radiographs, in which the tooth was submitted to conventional endodontic treatment. The results obtained after five years of clinical and radiographic followup demonstrated that conventional endodontic treatment is a clinically viable alternative in cases of type III dens invaginatus.

Methodological model of chronic stress associated with ligature-induced periodontitis in rats: a radiographic study.

This study evaluated the time efficiency of stress associated with ligature-induced periodontitis in rats. Sixty adult Wistar rats, housed in temperature-controlled rooms and receiving water and food ad libitum, were randomly separated into stress (n = 30) or control groups (n = 30). All animals were anesthetized, and nylon ligatures were placed at the gingival margin level of the maxillary right second molars.