Publications by authors named "Fabio Firmbach Pasqualotto"

Critical challenges for the public and private health, research, and medical communities have been posed by the COVID-19 outbreak. Some of these challenges are related to the possible adverse effects of SARS-CoV-2 on male reproductive health, and whether other potential modes of transmission may occur, such as sexual transmission. Moreover, concerns have been raised in terms of whether the COVID-19 outbreak may have an impact on fertility worldwide.

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An azoospermic man suffers from an absence of sperm in the ejaculate and this condition is present in about 10% of infertile men. Obstructive azoospermia (OA) is characterized by an occlusion or partial absence of the reproductive tract with the presence of normal spermatogenesis. On the other hand, non-obstructive azoospermia (NOA) is characterized by impaired spermatogenesis.

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This cohort study evaluated whether rescue spontaneous maturation (RSM) could be a valid method to increase the number of embryos available for transfer and whether transfers with RSM-derived embryos would contribute to clinical outcomes of poor-responder patients in ovarian stimulation cycles. The study included 440 patients undergoing intracytoplasmic sperm injection cycles in which fewer than five metaphase II (MII) oocytes and at least one immature oocyte were retrieved after follicle aspiration. Patients were allocated into two groups based on the injected oocytes' nuclear maturation status: MII group (n=330), in which only embryos derived from MII oocytes were transferred, and RSM group (n=110), in which at least one embryo derived from an RSM oocyte was transferred.

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Article Synopsis
  • The study investigates how the environment surrounding eggs, particularly the follicular fluid, influences fertilization and embryo development in couples undergoing Intracytoplasmic Sperm Injection (ICSI).
  • It analyzes oxidative stress markers—specifically superoxide dismutase (SOD), catalase, and lipid peroxidation (LPO)—in the semen, blood serum, and follicular fluid of 208 infertile couples.
  • Findings indicate high levels of follicular fluid SOD and catalase correlate with fertilization and cleavage rates, while increased LPO in follicular fluid is negatively linked to pregnancy rates, suggesting it could reflect metabolic activity necessary for pregnancy.
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While the idea of freezing human male gametes has been experimented since the late 1700's, it was not until 50 years ago that human sperm were frozen, and later thawed in such a way that they could fertilize an egg and initiate development. In the past several decades the technology of cryopreservation, or maintaining life in a frozen state, has advanced considerably. With the use of modern techniques, cryopreservation of sperm to preserve an individual's ability to reproduce has become successful, safe, and widely available.

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Objective: To evaluate the effect of male age on clinical outcomes of intracytoplasmic sperm injection (ICSI) cycles, according to sperm concentration.

Design: Retrospective, observational study.

Setting: Assisted reproduction center.

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Purpose: the propose of this study was to analyze the clinical and laboratorial parameters of patients submitted to human assisted reproduction techniques with association of sperm processing techniques, in order to remove virus particles from semen samples of couples in which men was infected by human immunodeficiency virus (HIV).

Methods: assessed were 11 intracytoplasmatic sperm injection (ICSI) cycles from couples whose men were HIV seropositive (HIV Group), and 35 cycles in which semen donors' samples were used in ICSI procedures (Control Group). Semen samples from Control Group were submitted to routine semen analysis, sperm wash and cryopreservation.

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Article Synopsis
  • The term "biological clock" typically refers to the age-related decline in fertility and hormone levels in women, but emerging research indicates that men also experience similar consequences as they age.
  • Studies show that the chances of a couple taking over a year to conceive almost doubles as male age increases, highlighting the significance of paternal age in fertility assessments.
  • Additionally, older men face greater risks of fertility decline and related health issues, making it crucial for patients and healthcare providers to recognize the impact of the male biological clock on reproductive and overall health.
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Article Synopsis
  • The study presents three cases of men who experienced azoospermia (absence of sperm) after taking clomiphene citrate (CC) for previously diagnosed oligospermia (low sperm count).
  • The patients initially had low sperm concentrations and showed no sperm in their semen analyses following CC treatment.
  • After discontinuing CC for three months, their sperm concentration improved slightly but remained below normal levels, highlighting the potential risks of using CC.
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Objective: To evaluate the effect of cigarette smoking on antioxidant levels and the presence of leukocytospermia in infertile men.

Design: Prospective study.

Setting: Academic medical center.

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Objective: To study the effect of freeze-thaw on embryos derived from intracytoplasmic sperm injection (ICSI) using surgically retrieved and ejaculated spermatozoa.

Design: Retrospective study.

Setting: Private IVF center.

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Objective: To evaluate the relationship between spindle visualization and intracytoplasmic sperm injection (ICSI) outcomes in controlled ovarian stimulation (COS) cycles.

Design: Prospective study.

Setting: Assisted reproduction center.

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Objective: To examine whether the presence of birefringent spindles in living human oocytes can be used as a predictive factor associated with embryo morphology to allow embryo selection before transfer and its association with IVF outcomes.

Design: Prospective study.

Setting: Assisted reproduction center in Brazil.

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Vasectomy is the method most commonly used in men for voluntary sterilization purposes. We report two cases of early recanalization following vasectomies performed in 1085 men for sterilization purposes at a tertiary public institution between January 2000 and November 2003. Thus, the risk of 0.

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Objective: To compare semen quality and levels of seminal oxidative stress among three groups: infertile men with varicocele, fertile men with varicocele, and healthy semen donors (controls) without varicocele.

Design: Prospective study.

Setting: Academic medical centers.

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Objective: To evaluate whether swine oocytes are useful for training new technicians in a human reproduction laboratory.

Design: Prospective study.

Setting: Graduate school in assisted reproductive techniques (ART) in Brazil.

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Objective: To assess fertilization, implantation, and pregnancy rates in patients undergoing ICSI using fresh and cryopreserved sperm from ejaculated semen samples.

Design: Retrospective study.

Setting: Academic and private medical centers.

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Article Synopsis
  • The study evaluated semen characteristics, testicular volume, and hormone levels in 889 normal fertile men seeking vasectomy.
  • A significant proportion of patients (87.2%) showed abnormal sperm morphology compared to World Health Organization (WHO) standards, with variations in other semen parameters among 6% to 32.2% of patients.
  • Results indicated that longer sexual abstinence increased semen volume and sperm concentration but decreased sperm motility, suggesting a need to reassess WHO normal values for sperm quality.
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Article Synopsis
  • The study aimed to evaluate how varicocele repair affects the fertility outcomes of azoospermic men and how these outcomes are related to testicular tissue analysis.
  • A total of 27 azoospermic men underwent both testicular biopsies and microsurgical varicocele repair, with semen analyses conducted before and after the procedures.
  • The results showed that about one-third of the men experienced improved sperm production following the surgery, though some returned to azoospermia within six months; one patient even managed to conceive.
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Introduction: It was the aim of this study to assess whether the changes in the diagnostic techniques and treatment modalities have altered the epidemiology of male factor infertility in the last decade.

Material And Methods: From September 1999 to July 2003, 822 patients were evaluated for infertility in a University Hospital. We divided our infertility patients according to the clinical diagnosis.

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