Publications by authors named "Fabio B Frederico"
Article Synopsis
- Ocular toxoplasmosis (OT) is an eye condition caused by a parasite that leads to inflammation of the retina and choroid, affecting vision; genetic variations in cytokine genes may influence susceptibility to this disease.
- A study categorized Brazilian participants with positive toxoplasmosis serology into two groups based on the presence or absence of OT, along with a control group without infection, to examine specific gene polymorphisms' roles.
- Findings indicated that individuals with the C/C genotype of a particular gene variant were less likely to develop OT, suggesting that this genetic factor may offer protection against the condition in the studied population.
Ocular toxoplasmosis (OT) is characterised by intraocular inflammation due to Toxoplasma gondii infection. Studies have found that interleukin 17 (IL-17) plays a central role in the pathology of OT. However, nucleotide variability in IL17 and interleukin 17 receptor (IL17R) genes has not been characterised in OT.
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- - The study investigates the relationship between Duffy blood group phenotypes and T. gondii infection in 576 patients, exploring whether Duffy antigens influence the invasion of the parasite.
- - Results indicate that T. gondii infection was more common in patients with the disease (22.9% presence) and those with positive anti-T. gondii IgG antibodies compared to those without the infection; however, no significant association was found with Duffy phenotypes.
- - Ultimately, the findings suggest that Duffy blood group phenotypes and their antigens do not increase the risk for T. gondii infection or the associated disease (OT).
Evaluation of Serological and Molecular Tests Used for the Identification of Infection in Patients Treated in an Ophthalmology Clinic of a Public Health Service in São Paulo State, Brazil.
Front Cell Infect Microbiol
September 2020
Article Synopsis
- Ocular toxoplasmosis is a common infection that can lead to serious eye problems, particularly prevalent in Brazil.
- The study compared various diagnostic methods (ELISA, ELFA, cPCR, Nested PCR, and qPCR) on patient groups with and without toxoplasmosis-related eye lesions.
- Results showed that while ELISA was more sensitive and ELFA more specific for serological diagnosis, qPCR had higher sensitivity but lower specificity than the other molecular methods.
Ocular toxoplasmosis (OT) is one of the most common manifestations of Toxoplasma gondii infection and can be related with congenital or acquired infections. OT cause posterior uveitis that cause serious sequelae as complete loss of vision. microRNAs (miRNAs) are small non-coding RNAs, which have regulatory roles in cells by silencing messenger RNA.
View Article and Find Full Text PDFOcular toxoplasmosis, caused by Toxoplasma gondii, is the most common cause of inflammation in the back of the eye. Analysis of the infecting strain may provide information regarding disease behavior and recurrence. Here, we describe clinical and epidemiological data for toxoplasmic retinochoroiditis in two Brazilian women infected by T.
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- CCR5 is a chemokine receptor linked to immune responses against infections, and this study investigates its association with ocular toxoplasmosis using two genetic polymorphisms (CCR5Δ32 and CCR5 59029 A/G).
- The study involved 480 patients divided into three groups based on the presence of ocular toxoplasmosis, and the analysis revealed significant age differences among these groups.
- Results indicated that individuals with both the CCR5/CCR5 genotype and CCR5-59029 AA or AG genotypes have a higher risk (4% more) of developing ocular toxoplasmosis, suggesting a link to a strong inflammatory response in the eyes.
Molecular diagnosis of symptomatic toxoplasmosis: a 9-year retrospective and prospective study in a referral laboratory in São Paulo, Brazil.
Braz J Infect Dis
January 2018
Article Synopsis
- Symptomatic toxoplasmosis affects about 10-20% of those infected, posing significant public health issues, especially in Brazil.
- This study aimed to enhance the molecular diagnosis of symptomatic toxoplasmosis by comparing the efficacy of two primer sets (B1 and REP-529) in detecting Toxoplasma gondii DNA through real-time PCR in 807 clinical samples.
- The results showed that REP-529 was more sensitive, detecting T. gondii DNA in 97.23% of positive samples, compared to 78.80% for B1, thereby supporting its use as a more effective diagnostic tool.
The objective of this study was to investigate the influence of the genes encoding the KIR receptors and their HLA ligands in the susceptibility of ocular toxoplasmosis. A total of 297 patients serologically-diagnosed with toxoplasmosis were selected and stratified according to the presence (n = 148) or absence (n = 149) of ocular scars/lesions due to toxoplasmosis. The group of patients with scars/lesions was further subdivided into two groups according to the type of ocular manifestation observed: primary (n = 120) or recurrent (n = 28).
View Article and Find Full Text PDFWITHDRAWN: Selection of reference genes in five types of human tissues for normalization of gene expression studies in infectious diseases.
Gene
October 2016
This article has been withdrawn at the request of the author(s) and/or editor. The Publisher apologizes for any inconvenience this may cause. The full Elsevier Policy on Article Withdrawal can be found at http://www.
View Article and Find Full Text PDFThis study investigated whether polymorphisms of the MICA (major histocompatibility complex class I chain-related gene A) gene are associated with eye lesions due to Toxoplasma gondii infection in a group of immunocompetent patients from southeastern Brazil. The study enrolled 297 patients with serological diagnosis of toxoplasmosis. Participants were classified into two distinct groups after conducting fundoscopic exams according to the presence (n = 148) or absence (n = 149) of ocular scars/lesions due to toxoplasmosis.
View Article and Find Full Text PDFBackground: Toxoplasmosis was recently included as a neglected disease by the Center for Disease Control. Ocular toxoplasmosis is one clinical presentation of congenital or acquired infection. The laboratory diagnosis is being used worldwide to support the clinical diagnosis and imaging.
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- The research focused on the synthesis of key cytokines (IFN-γ, TNF-α, IL-10) in patients with chronic Toxoplasma gondii infections who developed cerebral or ocular toxoplasmosis, comparing them to healthy individuals and those with chronic infections.
- It found that patients with advanced disease (CT/AIDS and OT groups) had lower levels of IFN-γ, suggesting reduced immunity against the parasite, while higher levels of TNF-α were associated with inflammation and damage in the affected tissues.
- Additionally, IL-10 levels were lower in diseased patients compared to those with chronic infections, indicating an immune response shift that may help the parasite
This prospective study evaluated the value of laboratorial diagnosis in ocular toxoplasmosis analyzing peripheral blood samples from a group of Brazilian patients by immunologic and molecular methods. We analyzed blood samples from 184 immunocompetent patients with ocular disorders divided into 2 groups: Group I, composed of samples from 49 patients with ocular toxoplasmosis diagnosed by clinical features; Group II, samples from 135 patients with other ocular diseases. Samples were assayed by conventional polymerase chain reaction (cnPCR), real-time PCR (qPCR) for Toxoplasma gondii, indirect immunofluorescence reaction (IF), avidity test (crude tachyzoite lysate as antigen), and excreted-secreted tachyzoite proteins as antigen (ESA-ELISA).
View Article and Find Full Text PDFPurpose: To evaluate the effectiveness of mitomycin C (MMC) in preventing recurrence of pterygium following conjunctival autograft transplantation (CAT). Ki-67 antigen to evaluate epithelial cell proliferation and fibroblast nuclear kariometry were used to assist treatment evaluation.
Methods: Twenty-nine patients with recurrent pterygium were divided into three groups: Group (G) 1--CAT and placebo eyedrops (PED); G2--CAT, 0.